Wisp2 disruption represses Cxcr4 expression and inhibits BMSCs homing to injured liver

doi:10.18632/oncotarget.22006

	Wisp2 disruption represses Cxcr4 expression and inhibits BMSCs homing to injured liver
	Qin, Dan 1; Yan, Yi 1; Hu, Bian 2; Zhang, Wanpo 1; Li, Hanmin 3; Li, Xiaodong 3; Liu, Shenghui 1; Dai, Depeng 1; Hu, Xiongji 1; Huang, Xingxu2 ; Zhang, Lisheng 1
	2017-11-17
发表期刊	ONCOTARGET
ISSN	1949-2553
卷号	8 期号:58 页码:98823-98836
发表状态	已发表
DOI	10.18632/oncotarget.22006
摘要	Liver regeneration/repair is a compensatory regrowth following acute liver failure, and bone marrow-derived mesenchyme stem cell (BMSC) transplantation is an effective therapy that promotes liver regeneration/repair. Wnt1 inducible signaling pathway protein 2 (Wisp2) is highly expressed in BMSCs, however, its function remains unclear. In this work, we used clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein -9 nuclease (CRISPR/Cas9) genome editing technology to knockdown Wisp2 in BMSCs, and these modified cells were then transplanted into rats which were induced by the 2-AAF/PH. By linking the expression of Cas9 to green fluorescent protein (GFP), we tracked BMSCs in the rats. Disruption of Wisp2 inhibited the homing of BMSCs to injured liver and aggravated liver damage as indicated by remarkably high levels of ALT and AST. Moreover, the key factor in BMSC transplantation, C-X-C chemokine receptor type 4 (Cxcr4), was down-regulated in the Wisp2 depleted BMSCs and had a lower expression in the livers of the corresponding rats. By tracing the GFP marker, more BMSCs were observed to differentiate into CD31 positive endothelial cells in the functional Wisp2 cells but less in the Wisp2 gene disrupted cells. In summary, Wisp2 promotes the homing of BMSCs through Cxcr4 related signaling during liver repair in rats.
关键词	rat Wisp2 Cxcr4 BMSCs homing
收录类别	SCI
语种	英语
资助项目	Fundamental Research Funds for the Central Universities[2662017PY106] ; Fundamental Research Funds for the Central Universities[2662016PY087]
WOS研究方向	Oncology ; Cell Biology
WOS类目	Oncology ; Cell Biology
WOS记录号	WOS:000419392300087
出版者	IMPACT JOURNALS LLC
WOS关键词	MARROW STROMAL CELLS ; STEM-CELLS ; GROWTH-FACTOR ; MIGRATION ; CANCER ; GENE ; TRANSPLANTATION ; PATHWAY ; TISSUE
原始文献类型	Article
引用统计
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/16294
专题	生命科学与技术学院_PI研究组_黄行许组
通讯作者	Zhang, Lisheng
作者单位	1.Univ Huazhong Agr, Coll Vet Med, Wuhan 430070, Hubei, Peoples R China 2.Shanghai Tech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China 3.Hubei Prov Hosp Tradit Chinese Med, Hepat Dis Inst, Wuhan 430061, Hubei, Peoples R China
推荐引用方式 GB/T 7714	Qin, Dan,Yan, Yi,Hu, Bian,et al. Wisp2 disruption represses Cxcr4 expression and inhibits BMSCs homing to injured liver[J]. ONCOTARGET,2017,8(58):98823-98836.
APA	Qin, Dan.,Yan, Yi.,Hu, Bian.,Zhang, Wanpo.,Li, Hanmin.,...&Zhang, Lisheng.(2017).Wisp2 disruption represses Cxcr4 expression and inhibits BMSCs homing to injured liver.ONCOTARGET,8(58),98823-98836.
MLA	Qin, Dan,et al."Wisp2 disruption represses Cxcr4 expression and inhibits BMSCs homing to injured liver".ONCOTARGET 8.58(2017):98823-98836.