Integrative Proteomics-Metabolomics Strategy for Pathological Mechanism of Vascular Depression Mouse Model

doi:10.1021/acs.jproteome.7b00724

	Integrative Proteomics-Metabolomics Strategy for Pathological Mechanism of Vascular Depression Mouse Model
	Zhao, Hongxia 1; Du, Hongli 2; Liu, Min 3; Gao, Songyan 1; Li, Na 1; Chao, Yufan 1; Li, Ruiqing 4; Chen, Wei 5; Lou, Ziyang 1; Dong, Xin 1
	2018-01
发表期刊	JOURNAL OF PROTEOME RESEARCH (IF:3.8[JCR-2023],4.1[5-Year])
ISSN	1535-3893
卷号	17 期号:1 页码:656-669
发表状态	已发表
DOI	10.1021/acs.jproteome.7b00724
摘要	Vascular depression (VD), a subtype of depression, is caused by vascular diseases or cerebrovascular risk factors. Recently, the proportion of VD patients has increased significantly, which severely affects their quality of life. However, the current pathogenesis of VD has not yet been fully understood, and the basic research is not adequate. In this study, on the basis of the combination of LC MS-based proteomics and metabolomics, we aimed to establish a protein metabolism regulatory network in a murine VD model to elucidate a more comprehensive impact of VD on organisms. We detected 44 metabolites and 304 proteins with different levels in the hippocampus samples from VD mice using a combination of metabolomic and proteomics analyses, with an isobaric tags for relative and absolute quantification (iTRAQ) method. We constructed a protein to-metabolic regulatory network by correlating and integrating the differential metabolites and proteins using ingenuity pathway analysis. Then we quantitatively validated the levels of the bimolecules shown in the bioinformatics analysis using LC-MS/MS and Western blotting. Validation results suggested changes in the regulation of neuroplasticity, transport of neurotransmitters, neuronal cell proliferation and apoptosis, and disorders of amino acids, lipids and energy metabolism. These proteins and metabolites involved in these dis-regulated pathways will provide a more targeted and credible direction to study the mechanism of VD. Therefore, this paper presents an approach and strategy that was applied in integrative proteomics and metabolomics for research and screening potential targets and biomarkers of VD, which could be more precise and credible in a field lacking adequate basic research.
关键词	proteomics metabolomics vascular depression LC-MS
收录类别	SCI ; SCIE ; SSCI
语种	英语
资助项目	Military Medical Innovation Project[16CXZ012]
WOS研究方向	Biochemistry & Molecular Biology
WOS类目	Biochemical Research Methods
WOS记录号	WOS:000419749800059
出版者	AMER CHEMICAL SOC
WOS关键词	LONG-TERM POTENTIATION ; GLOBAL CEREBRAL-ISCHEMIA ; GLUTAMATE RECEPTORS ; RISK-FACTOR ; RAT MODEL ; DISEASE ; MICE ; EPIDEMIOLOGY ; DEFICITS ; ANTIDEPRESSANTS
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/16290
专题	生命科学与技术学院
通讯作者	Chen, Wei; Lou, Ziyang; Dong, Xin
作者单位	1.Second Mil Med Univ, Sch Pharm, Shanghai 200433, Peoples R China 2.Eastern Hepatobiliary Surg Hosp, Dept Pharm, Shanghai 200433, Peoples R China 3.Second Mil Med Univ, Changhai Hosp, Pharm Dept, Shanghai 200433, Peoples R China 4.Shanghai Tech Univ, Sch Life Sci & Technol, Shanghai 200433, Peoples R China 5.Second Mil Med Univ, Changhai Hosp, Shanghai 200433, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Hongxia,Du, Hongli,Liu, Min,et al. Integrative Proteomics-Metabolomics Strategy for Pathological Mechanism of Vascular Depression Mouse Model[J]. JOURNAL OF PROTEOME RESEARCH,2018,17(1):656-669.
APA	Zhao, Hongxia.,Du, Hongli.,Liu, Min.,Gao, Songyan.,Li, Na.,...&Dong, Xin.(2018).Integrative Proteomics-Metabolomics Strategy for Pathological Mechanism of Vascular Depression Mouse Model.JOURNAL OF PROTEOME RESEARCH,17(1),656-669.
MLA	Zhao, Hongxia,et al."Integrative Proteomics-Metabolomics Strategy for Pathological Mechanism of Vascular Depression Mouse Model".JOURNAL OF PROTEOME RESEARCH 17.1(2018):656-669.