GroEL Ring Separation and Exchange in the Chaperonin Reaction

doi:10.1016/j.cell.2017.12.010

	GroEL Ring Separation and Exchange in the Chaperonin Reaction
	Yan, Xiao 1; Shi, Qiaoyun1,2 ; Bracher, Andreas 1; Milicic, Goran 1; Singh, Amit K.1; Hartl, F. Ulrich 1; Hayer-Hartl, Manajit 1
	2018-01-25
发表期刊	CELL (IF:45.5[JCR-2023],49.0[5-Year])
ISSN	0092-8674
卷号	172 期号:3 页码:605-+
发表状态	已发表
DOI	10.1016/j.cell.2017.12.010
摘要	The bacterial chaperonin GroEL and its cofactor, GroES, form a nano-cage for a single molecule of substrate protein (SP) to fold in isolation. GroEL and GroES undergo an ATP-regulated interaction cycle to close and open the folding cage. GroEL consists of two heptameric rings stacked back to back. Here, we show that GroEL undergoes transient ring separation, resulting in ring exchange between complexes. Ring separation occurs upon ATP-binding to the trans ring of the asymmetric GroEL:7ADP:GroES complex in the presence or absence of SP and is a consequence of inter-ring negative allostery. We find that a GroEL mutant unable to perform ring separation is folding active but populates symmetric GroEL: GroES(2) complexes, where both GroEL rings function simultaneously rather than sequentially. As a consequence, SP binding and release from the folding chamber is inefficient, and E. coli growth is impaired. We suggest that transient ring separation is an integral part of the chaperonin mechanism.
收录类别	SCI ; SCIE
语种	英语
资助项目	Deutsche Forschungsgemeinschaft[SFB 1035]
WOS研究方向	Biochemistry & Molecular Biology ; Cell Biology
WOS类目	Biochemistry & Molecular Biology ; Cell Biology
WOS记录号	WOS:000423447600018
出版者	CELL PRESS
WOS关键词	FOLDING FUNCTIONAL FORM ; CRYSTAL-STRUCTURE ; ATP HYDROLYSIS ; REACTION CYCLE ; IN-VIVO ; POLYPEPTIDE BINDING ; ESCHERICHIA-COLI ; HEAT-SHOCK ; NANO-CAGE ; PROTEIN
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/16275
专题	iHuman研究所_公共科研平台_动物细胞培养和蛋白纯化平台 iHuman研究所_公共科研平台_Assay及筛选平台
通讯作者	Hartl, F. Ulrich; Hayer-Hartl, Manajit
作者单位	1.Max Planck Inst Biochem, Dept Cellular Biochem, Klopferspitz 18, D-82152 Martinsried, Germany 2.ShanghaiTech Univ, IHuman Inst, Shanghai 201210, Peoples R China
推荐引用方式 GB/T 7714	Yan, Xiao,Shi, Qiaoyun,Bracher, Andreas,et al. GroEL Ring Separation and Exchange in the Chaperonin Reaction[J]. CELL,2018,172(3):605-+.
APA	Yan, Xiao.,Shi, Qiaoyun.,Bracher, Andreas.,Milicic, Goran.,Singh, Amit K..,...&Hayer-Hartl, Manajit.(2018).GroEL Ring Separation and Exchange in the Chaperonin Reaction.CELL,172(3),605-+.
MLA	Yan, Xiao,et al."GroEL Ring Separation and Exchange in the Chaperonin Reaction".CELL 172.3(2018):605-+.