Synaptic Targeting and Function of SAPAPs Mediated by Phosphorylation-Dependent Binding to PSD-95 MAGUKs

doi:10.1016/j.celrep.2017.11.107

	Synaptic Targeting and Function of SAPAPs Mediated by Phosphorylation-Dependent Binding to PSD-95 MAGUKs
	Zhu, Jinwei 1,2,3; Zhou, Qingqing 3; Shang, Yuan 3; Li, Hao 4,5,6; Peng, Mengjuan1,2,7 ; Ke, Xiao 4,5,6; Weng, Zhuangfeng1,2,7 ; Zhang, Rongguang1,2,7 ; Huang, Xuhui 8,12; Li, Shawn S. C.9; Feng, Guoping 10,11; Lu, Youming 4,5,6; Zhang, Mingjie 3,12
	2017-12-26
发表期刊	CELL REPORTS (IF:7.5[JCR-2023],8.5[5-Year])
ISSN	2211-1247
卷号	21 期号:13 页码:3781-3793
发表状态	已发表
DOI	10.1016/j.celrep.2017.11.107
摘要	The PSD-95/SAPAP/Shank complex functions as the major scaffold in orchestrating the formation and plasticity of the post-synaptic densities (PSDs). We previously demonstrated that the exquisitely specific SAPAP/Shank interaction is critical for Shank synaptic targeting and Shank-mediated synaptogenesis. Here, we show that the PSD-95/SAPAP interaction, SAPAP synaptic targeting, and SAPAP-mediated synaptogenesis require phosphorylation of the N-terminal repeat sequences of SAPAPs. The atomic structure of the PSD-95 guanylate kinase (GK) in complex with a phosphor-SAPAP repeat peptide, together with biochemical studies, reveals the molecular mechanism underlying the phosphorylation-dependent PSD-95/SAPAP interaction, and it also provides an explanation of a PSD-95 mutation found in patients with intellectual disabilities. Guided by the structural data, we developed potent non-phosphorylated GK inhibitory peptides capable of blocking the PSD-95/SAPAP interaction and interfering with PSD-95/SAPAP-mediated synaptic maturation and strength. These peptides are genetically encodable for investigating the functions of the PSD-95/SAPAP interaction in vivo.
收录类别	SCI
语种	英语
资助项目	973 program grant from Minister of Science and Technology of China[2014CB910204]
WOS研究方向	Cell Biology
WOS类目	Cell Biology
WOS记录号	WOS:000418721800015
出版者	CELL PRESS
WOS关键词	POSTSYNAPTIC DENSITY PROTEINS ; AUTISM SPECTRUM DISORDERS ; KINASE-ASSOCIATED PROTEIN ; DENDRITIC SPINES ; AMPA RECEPTORS ; INTELLECTUAL DISABILITY ; PSYCHIATRIC-DISORDERS ; SCAFFOLDING PROTEINS ; EXCITATORY SYNAPSES ; RECOGNITION MODE
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/16215
专题	生命科学与技术学院生命科学与技术学院_特聘教授组_张荣光组生命科学与技术学院_硕士生生命科学与技术学院_博士生
通讯作者	Zhang, Mingjie
作者单位	1.Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol, Natl Ctr Prot Sci Shanghai, CAS Ctr Excellence Mol Cell Sci, Shanghai 201203, Peoples R China 2.Univ Chinese Acad Sci, 333 Haike Rd, Shanghai 201203, Peoples R China 3.Hong Kong Univ Sci & Technol, Div Life Sci, State Key Lab Mol Neurosci, Kowloon, Hong Kong, Peoples R China 4.Huazhong Univ Sci & Technol, Sch Basic Med, Dept Physiol, Wuhan 4030030, Hubei, Peoples R China 5.Huazhong Univ Sci & Technol, Tongji Med Coll, Wuhan 4030030, Hubei, Peoples R China 6.Huazhong Univ Sci & Technol, Inst Brain Res, Collaborat Innovat Ctr Brain Sci, Wuhan 430030, Hubei, Peoples R China 7.ShanghaiTech Univ, Sch Life Sci & Technol, 100 Haike Rd, Shanghai 201210, Peoples R China 8.Hong Kong Univ Sci & Technol, Dept Chem, Kowloon, Hong Kong, Peoples R China 9.Univ Western Ontario, Siebens Drake Med Res Inst, Schulich Sch Med & Dent, Dept Biochem, London, ON N6A 5C1, Canada 10.MIT, Dept Brain & Cognit Sci, McGovern Inst Brain Res, E25-618, Cambridge, MA 02139 USA 11.Broad Inst MIT & Harvard, Stanley Ctr Psychiat Res, Cambridge, MA 02142 USA 12.Hong Kong Univ Sci & Technol, Ctr Syst Biol & Human Hlth, Kowloon, Hong Kong, Peoples R China
推荐引用方式 GB/T 7714	Zhu, Jinwei,Zhou, Qingqing,Shang, Yuan,et al. Synaptic Targeting and Function of SAPAPs Mediated by Phosphorylation-Dependent Binding to PSD-95 MAGUKs[J]. CELL REPORTS,2017,21(13):3781-3793.
APA	Zhu, Jinwei.,Zhou, Qingqing.,Shang, Yuan.,Li, Hao.,Peng, Mengjuan.,...&Zhang, Mingjie.(2017).Synaptic Targeting and Function of SAPAPs Mediated by Phosphorylation-Dependent Binding to PSD-95 MAGUKs.CELL REPORTS,21(13),3781-3793.
MLA	Zhu, Jinwei,et al."Synaptic Targeting and Function of SAPAPs Mediated by Phosphorylation-Dependent Binding to PSD-95 MAGUKs".CELL REPORTS 21.13(2017):3781-3793.