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Segmental Isotopic Labeling of Proteins for NMR Study Using Intein Technology | |
2017 | |
来源专著 | Methods in Molecular Biology |
出版地 | 999 RIVERVIEW DR, STE 208, TOTOWA, NJ 07512-1165 USA |
出版者 | HUMANA PRESS INC |
ISSN | 1064-3745 |
卷号 | 1495 |
页码 | 131-145 |
摘要 | Segmental isotopic labeling of samples for NMR studies is attractive for large complex biomacromolecular systems, especially for studies of function-related protein-ligand interactions and protein dynamics (Goto and Kay, Curr Opin Struct Biol 10: 585-592, 2000; Rosa et al., Molecules (Basel, Switzerland) 18: 440, 2013; Hiroaki, Expert Opin Drug Discovery 8: 523-536, 2013). Advantages of segmental isotopic labeling include selective examination of specific segment(s) within a protein by NMR, significantly reducing the spectral complexity for large proteins, and allowing for the application of a variety of solution-based NMR strategies. By utilizing intein techniques (Wood and Camarero, J Biol Chem 289: 14512-14519, 2014; Paulus, Annu Rev Biochem 69: 447-496, 2000), two related approaches can generally be used in the segmental isotopic labeling of proteins: expressed protein ligation (Muir, Annu Rev Biochem 72: 249289, 2003) and protein trans - splicing (Shah et al., J Am Chem Soc 134: 11338-11341, 2012). Here, we describe general implementation and latest improvements of expressed protein ligation method for the production of segmental isotopic labeled NMR samples. |
关键词 | Expressed protein ligation Isotopic labeling Intein Structural biology NMR |
DOI | 10.1007/978-1-4939-6451-2_9 |
收录类别 | BSCI |
语种 | 英语 |
WOS记录号 | WOS:000400929800010 |
通讯作者 | Liu, Dongsheng |
WOS类目 | Biochemical Research Methods ; Biochemistry & Molecular Biology |
WOS研究方向 | Biochemistry & Molecular Biology |
原始文献类型 | Article ; Book Chapter |
引用统计 | 正在获取...
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文献类型 | 专著章节 |
条目标识符 | https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/1590 |
专题 | iHuman研究所_特聘教授组_Kurt Wuthrich组 |
通讯作者 | Liu, Dongsheng |
作者单位 | 1.ShanghaiTech Univ, iHuman Inst, Shanghai, Peoples R China 2.Albert Einstein Coll Med, Dept Biochem, Bronx, NY 10467 USA |
第一作者单位 | iHuman研究所 |
推荐引用方式 GB/T 7714 | Liu, Dongsheng,Cowburn, David. Segmental Isotopic Labeling of Proteins for NMR Study Using Intein Technology. 999 RIVERVIEW DR, STE 208, TOTOWA, NJ 07512-1165 USA:HUMANA PRESS INC,2017:131-145. |
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