GGPP-Mediated Protein Geranylgeranylation in Oocyte Is Essential for the Establishment of Oocyte-Granulosa Cell Communication and Primary-Secondary Follicle Transition in Mouse Ovary

doi:10.1371/journal.pgen.1006535

	GGPP-Mediated Protein Geranylgeranylation in Oocyte Is Essential for the Establishment of Oocyte-Granulosa Cell Communication and Primary-Secondary Follicle Transition in Mouse Ovary
	Jiang, Chen 1,2,3; Diao, Fan 1,2; Sang, Yong-Juan 1,2,3; Xu, Na 1,2; Zhu, Rui-Lou 1,2,3; Wang, Xiu-Xing 1,2; Chen, Zhong 1,2; Tao, Wei-Wei 1,2; Yao, Bing 3; Sun, Hai-Xiang 3; Huang, Xing-Xu4 ; Xue, Bin 1,2; Li, Chao-Jun 1,2,3
	2017-01
发表期刊	PLOS GENETICS
ISSN	1553-7404
卷号	13 期号:1
发表状态	已发表
DOI	10.1371/journal.pgen.1006535
摘要	Folliculogenesis is a progressive and highly regulated process, which is essential to provide ova for later reproductive life, requires the bidirectional communication between the oocyte and granulosa cells. This physical connection-mediated communication conveys not only the signals from the oocyte to granulosa cells that regulate their proliferation but also metabolites from the granulosa cells to the oocyte for biosynthesis. However, the underlying mechanism of establishing this communication is largely unknown. Here, we report that oocyte geranylgeranyl diphosphate (GGPP), a metabolic intermediate involved in protein geranyl-geranylation, is required to establish the oocyte-granulosa cell communication. GGPP and geranylgeranyl diphosphate synthase (Ggpps) levels in oocytes increased during early follicular development. The selective depletion of GGPP in mouse oocytes impaired the proliferation of granulosa cells, primary-secondary follicle transition and female fertility. Mechanistically, GGPP depletion inhibited Rho GTPase geranylgeranylation and its GTPase activity, which was responsible for the accumulation of cell junction proteins in the oocyte cytoplasm and the failure to maintain physical connection between oocyte and granulosa cells. GGPP ablation also blocked Rab27a geranylgeranylation, which might account for the impaired secretion of oocyte materials such as Gdf9. Moreover, GGPP administration restored the defects in oocyte-granulosa cell contact, granulosa cell proliferation and primary-secondary follicle transition in Ggpps depletion mice. Our study provides the evidence that GGPP-mediated protein geranylgeranylation contributes to the establishment of oocyte-granulosa cell communication and then regulates the primary-secondary follicle transition, a key phase of folliculogenesis essential for female reproductive function.
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[31530046] ; National Natural Science Foundation of China[31271540]
WOS研究方向	Genetics & Heredity
WOS类目	Genetics & Heredity
WOS记录号	WOS:000394147700013
出版者	PUBLIC LIBRARY SCIENCE
WOS关键词	GROWTH-DIFFERENTIATION FACTOR-9 ; GAP-JUNCTIONS ; METABOLIC COOPERATIVITY ; INTERCELLULAR COMMUNICATION ; MEIOTIC ARREST ; GTPASES ; RHO ; CADHERIN ; FOLLICULOGENESIS ; ACTIVATION
原始文献类型	Article
引用统计
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/1566
专题	生命科学与技术学院_PI研究组_黄行许组
通讯作者	Li, Chao-Jun
作者单位	1.Nanjing Univ, Natl Resource Ctr Mutant Mice, Model Anim Res Ctr, MOE Key Lab Model Anim Dis Study, Nanjing, Jiangsu, Peoples R China 2.Nanjing Univ, Natl Resource Ctr Mutant Mice, Sch Med, Nanjing, Jiangsu, Peoples R China 3.Nanjing Univ, Collaborat Innovat Platform Reprod Biol & Technol, Sch Med, Nanjing, Jiangsu, Peoples R China 4.Shanghai Tech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China
推荐引用方式 GB/T 7714	Jiang, Chen,Diao, Fan,Sang, Yong-Juan,et al. GGPP-Mediated Protein Geranylgeranylation in Oocyte Is Essential for the Establishment of Oocyte-Granulosa Cell Communication and Primary-Secondary Follicle Transition in Mouse Ovary[J]. PLOS GENETICS,2017,13(1).
APA	Jiang, Chen.,Diao, Fan.,Sang, Yong-Juan.,Xu, Na.,Zhu, Rui-Lou.,...&Li, Chao-Jun.(2017).GGPP-Mediated Protein Geranylgeranylation in Oocyte Is Essential for the Establishment of Oocyte-Granulosa Cell Communication and Primary-Secondary Follicle Transition in Mouse Ovary.PLOS GENETICS,13(1).
MLA	Jiang, Chen,et al."GGPP-Mediated Protein Geranylgeranylation in Oocyte Is Essential for the Establishment of Oocyte-Granulosa Cell Communication and Primary-Secondary Follicle Transition in Mouse Ovary".PLOS GENETICS 13.1(2017).