Mapping circular RNA structures in living cells by SHAPE-MaP

doi:10.1016/j.ymeth.2021.01.011

	Mapping circular RNA structures in living cells by SHAPE-MaP
	Guo, Si-Kun 1; Nan, Fang 2; Liu, Chu-Xiao 1; Yang, Li2,3 ; Chen, Ling-Ling1,3,4
	2021-12
发表期刊	METHODS (IF:4.2[JCR-2023],3.8[5-Year])
ISSN	1046-2023
EISSN	1095-9130
卷号	196
发表状态	已发表
DOI	10.1016/j.ymeth.2021.01.011
摘要	Circular RNAs are produced from back-splicing of exons of precursor mRNAs (pre-mRNAs). The sequences of exons in circular RNAs are identical to their linear cognate mRNAs, but the circular format may confer constraints on their folding and conformation, leading to potentially different functions from their linear RNA cognates. Here, we describe experimental and computational steps that optimize the selective 2'-hydroxyl acylation analyzed by primer extension and mutational profiling (SHAPE-MaP) to probe circular RNA secondary structure at single-nucleotide resolution in living cells.
关键词	Circular RNA Secondary structure Living cell SHAPE-MaP
URL	查看原文
收录类别	SCI ; SCIE
语种	英语
资助项目	National Natural Science Foundation of China (NSFC)[91940303,31725009,31821004] ; Chinese Academy of Sciences (CAS)[XDB19020104] ; HHMI Interna-tional Program[55008728]
WOS研究方向	Biochemistry & Molecular Biology
WOS类目	Biochemical Research Methods ; Biochemistry & Molecular Biology
WOS记录号	WOS:000744263600008
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/153561
专题	生命科学与技术学院_特聘教授组_杨力组生命科学与技术学院_特聘教授组_陈玲玲组
通讯作者	Chen, Ling-Ling
作者单位	1.Chinese Acad Sci, Univ Chinese Acad Sci, CAS Ctr Excellence Mol Cell Sci,Shanghai Key Lab, Shanghai Inst Biochem & Cell Biol,State Key Lab M, 320 Yueyang Rd, Shanghai 200031, Peoples R China 2.Chinese Acad Sci, Univ Chinese Acad Sci, Shanghai Inst Nutr & Hlth, CAS Key Lab Computat Biol, 320 Yueyang Rd, Shanghai 200031, Peoples R China 3.ShanghaiTech Univ, Sch Life Sci & Technol, 100 Haike Rd, Shanghai 201210, Peoples R China 4.Univ Chinese Acad Sci, Sch Life Sci, Hangzhou Inst Adv Study, Hangzhou 310024, Peoples R China
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Guo, Si-Kun,Nan, Fang,Liu, Chu-Xiao,et al. Mapping circular RNA structures in living cells by SHAPE-MaP[J]. METHODS,2021,196.
APA	Guo, Si-Kun,Nan, Fang,Liu, Chu-Xiao,Yang, Li,&Chen, Ling-Ling.(2021).Mapping circular RNA structures in living cells by SHAPE-MaP.METHODS,196.
MLA	Guo, Si-Kun,et al."Mapping circular RNA structures in living cells by SHAPE-MaP".METHODS 196(2021).