Downregulated miR-29a/b/c during Contact Inhibition Stage Promote 3T3-L1 Adipogenesis by Targeting DNMT3A

doi:10.1371/journal.pone.0170636

	Downregulated miR-29a/b/c during Contact Inhibition Stage Promote 3T3-L1 Adipogenesis by Targeting DNMT3A
	Zhu, Yingjie 1; Zheng, Guangyong 2; Wang, Huichao 1; Jia, Yudong 1; Zhang, Ying 1; Tang, Yanfeng 1; Li, Wenlong 1; Fan, Yanan 1; Zhang, Xiaodong 1; Liu, Youwen 1; Liu, Sanhong3
	2017-01-23
发表期刊	PLOS ONE (IF:2.9[JCR-2023],3.3[5-Year])
ISSN	1932-6203
卷号	12 期号:1
发表状态	已发表
DOI	10.1371/journal.pone.0170636
摘要	Differentiation of 3T3-L1 cells into adipocytes involves a highly-orchestrated series of events including contact inhibition (CI), clonal expansion, growth arrest, and terminal differentiation. Recent study demonstrated that 3T3-L1 preadipocytes will not be differentiated into mature adipocytes without CI stage, which indicated that CI stage plays an important role during 3T3-L1 adipogenesis. However, the molecular mechanism is not yet fully understood. In the present study, we found that the expression level of miR-29a/b/c was decreased and the expression of DNMT3A was up-regulated during CI stage, respectively. Furthermore, overexpression of miR-29a/b/c during CI stage inhibits adipogenesis significantly but not at other stages. In addition, miR-29a/b/c repressed DNMT3A expression by directly targeting its 3' untranslated region (3' UTR). Our data reveal a novel mechanism of miR-29a/b/c in the regulation of adipogenesis.
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[81473704]
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000396129000081
出版者	PUBLIC LIBRARY SCIENCE
WOS关键词	MESENCHYMAL STEM-CELLS ; HUMAN ADIPOSE-TISSUE ; ADIPOCYTE DIFFERENTIATION ; UP-REGULATION ; MICRORNAS ; ROLES ; RATS
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/1531
专题	免疫化学研究所_特聘教授组_干细胞生物学实验室
通讯作者	Liu, Sanhong
作者单位	1.Henan Prov Orthoped Hosp, Henan Prov Luoyang Orthoped Traumatol Hosp, Luoyang, Henan, Peoples R China 2.Chinese Acad Sci, CAS MPG Partner Inst Computat Biol, Shanghai, Peoples R China 3.ShanghaiTech Univ, Shanghai Inst Adv Immunochem Studies, Shanghai, Peoples R China
通讯作者单位	免疫化学研究所
推荐引用方式 GB/T 7714	Zhu, Yingjie,Zheng, Guangyong,Wang, Huichao,et al. Downregulated miR-29a/b/c during Contact Inhibition Stage Promote 3T3-L1 Adipogenesis by Targeting DNMT3A[J]. PLOS ONE,2017,12(1).
APA	Zhu, Yingjie.,Zheng, Guangyong.,Wang, Huichao.,Jia, Yudong.,Zhang, Ying.,...&Liu, Sanhong.(2017).Downregulated miR-29a/b/c during Contact Inhibition Stage Promote 3T3-L1 Adipogenesis by Targeting DNMT3A.PLOS ONE,12(1).
MLA	Zhu, Yingjie,et al."Downregulated miR-29a/b/c during Contact Inhibition Stage Promote 3T3-L1 Adipogenesis by Targeting DNMT3A".PLOS ONE 12.1(2017).