ShanghaiTech University Knowledge Management System
Quantitative proteomics reveal proteins enriched in tubular endoplasmic reticulum of Saccharomyces cerevisiae | |
2017 | |
发表期刊 | ELIFE |
ISSN | 2050-084X |
卷号 | 6页码:e23816 |
发表状态 | 已发表 |
DOI | 10.7554/elife.23816 |
摘要 | The tubular network is a critical part of the endoplasmic reticulum (ER). The network is shaped by the reticulons and REEPs/Yop1p that generate tubules by inducing high membrane curvature, and the dynamin-like GTPases atlastin and Sey1p/RHD3 that connect tubules via membrane fusion. However, the specific functions of this ER domain are not clear. Here, we isolated tubule-based microsomes from Saccharomyces cerevisiae via classical cell fractionation and detergent-free immunoprecipitation of Flag-tagged Yop1p, which specifically localizes to ER tubules. In quantitative comparisons of tubule-derived and total microsomes, we identified a total of 79 proteins that were enriched in the ER tubules, including known proteins that organize the tubular ER network. Functional categorization of the list of proteins revealed that the tubular ER network may be involved in membrane trafficking, lipid metabolism, organelle contact, and stress sensing. We propose that affinity isolation coupled with quantitative proteomics is a useful tool for investigating ER functions. |
收录类别 | SCI |
语种 | 英语 |
资助项目 | Ministry of Science and Tech-nology of the People's Republic of China[2016YFA0500201] |
WOS研究方向 | Life Sciences & Biomedicine - Other Topics |
WOS类目 | Biology |
WOS记录号 | WOS:000397631000001 |
出版者 | ELIFE SCIENCES PUBLICATIONS LTD |
WOS关键词 | HOMOTYPIC ER FUSION ; MEMBRANE CURVATURE ; STRUCTURAL BASIS ; PROVIDE INSIGHT ; CONTACT SITES ; ATLASTIN ; NETWORK ; GTPASES ; MECHANISMS ; DOMAINS |
原始文献类型 | Article |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/1473 |
专题 | iHuman研究所_PI研究组_水雯箐组 生命科学与技术学院 |
通讯作者 | Shui, Wenqing; Hu, Junjie |
作者单位 | 1.Nankai Univ, Dept Genet & Cell Biol, Coll Life Sci, Tianjin, Peoples R China 2.ShanghaiTech Univ, iHuman Inst, Shanghai, Peoples R China 3.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China 4.Huazhong Univ Sci & Technol, Coll Life Sci, Wuhan, Peoples R China 5.Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing, Peoples R China 6.Chinese Acad Sci, Inst Biophys, CAS Ctr Excellence Biomacromol, Beijing, Peoples R China |
通讯作者单位 | iHuman研究所; 生命科学与技术学院 |
推荐引用方式 GB/T 7714 | Wang, Xinbo,Li, Shanshan,Wang, Haicheng,et al. Quantitative proteomics reveal proteins enriched in tubular endoplasmic reticulum of Saccharomyces cerevisiae[J]. ELIFE,2017,6:e23816. |
APA | Wang, Xinbo,Li, Shanshan,Wang, Haicheng,Shui, Wenqing,&Hu, Junjie.(2017).Quantitative proteomics reveal proteins enriched in tubular endoplasmic reticulum of Saccharomyces cerevisiae.ELIFE,6,e23816. |
MLA | Wang, Xinbo,et al."Quantitative proteomics reveal proteins enriched in tubular endoplasmic reticulum of Saccharomyces cerevisiae".ELIFE 6(2017):e23816. |
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