Biallelic beta-carotene oxygenase 2 knockout results in yellow fat in sheep via CRISPR/Cas9
Niu, Y.1; Jin, M.1; Li, Y.1; Li, P.1; Zhou, J.2; Wang, X.1; Petersen, B.3; Huang, X.2; Kou, Q.4; Chen, Y.1
Source PublicationANIMAL GENETICS
AbstractThe recently emerged CRISPR/Cas9 approach represents an efficient and versatile genome editing tool for producing genetically modified animals. B-carotene oxygenase 2 (BCO2) is a key enzyme in the progress of beta-carotene metabolism and is associated with yellow adipose tissue color in sheep. We have recently demonstrated targeted multiplex mutagenesis in sheep and have generated a group of BCO2-disrupted sheep by zygote injection of the CRISPR/Cas9 components. Here, we show that biallelic modification of BCO2 resulted in yellow fat, compared with the fat color in monoallelic individuals and wild types (snowflower white). We subsequently characterized the effects of gene modifications at genetic levels employing sequencing and Western blotting, highlighting the importance of the BCO2 gene for the determination of fat color in sheep. These results indicate that genetic modification via CRISPR/Cas9 holds great potential for validating gene functions as well as for generating desirable phenotypes for economically important traits in livestock.
Keywordgenetic engineering genome editing Ovis aries phenotype beta-carotene oxygenase 2
Indexed BySCI
Funding ProjectChina Agriculture Research System[CARS-40-13]
WOS Research AreaAgriculture ; Genetics & Heredity
WOS SubjectAgriculture, Dairy & Animal Science ; Genetics & Heredity
WOS IDWOS:000396045600015
Original Document TypeArticle
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Cited Times:21[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Corresponding AuthorKou, Q.; Chen, Y.
Affiliation1.Northwest A&F Univ, Coll Anim Sci & Technol, Yangling 712100, Shaanxi, Peoples R China
2.Shanghai Tech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China
3.Friedrich Loeffler Inst, Inst Farm Anim Genet, D-31535 Neustadt, Germany
4.Ningxia Tianyuan Sheep Farm, Hongsibu 751999, Peoples R China
Recommended Citation
GB/T 7714
Niu, Y.,Jin, M.,Li, Y.,et al. Biallelic beta-carotene oxygenase 2 knockout results in yellow fat in sheep via CRISPR/Cas9[J]. ANIMAL GENETICS,2017,48(2):242-244.
APA Niu, Y..,Jin, M..,Li, Y..,Li, P..,Zhou, J..,...&Chen, Y..(2017).Biallelic beta-carotene oxygenase 2 knockout results in yellow fat in sheep via CRISPR/Cas9.ANIMAL GENETICS,48(2),242-244.
MLA Niu, Y.,et al."Biallelic beta-carotene oxygenase 2 knockout results in yellow fat in sheep via CRISPR/Cas9".ANIMAL GENETICS 48.2(2017):242-244.
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