Lipid-dependent conformational dynamics underlie the functional versatility of T-cell receptor

doi:10.1038/cr.2017.42

	Lipid-dependent conformational dynamics underlie the functional versatility of T-cell receptor
	Guo, Xingdong 1,2; Yan, Chengsong 1,2; Li, Hua 1,2; Huang, Wenmao 3,4; Shi, Xiaoshan 1,2; Huang, Min 1,2; Wang, Yingfang 1,2; Pan, Weiling 1,2; Cai, Mingjun 5; Li, Lunyi 1,2; Wu, Wei 1,2; Bai, Yibing 1,2; Zhang, Chi 1,2; Liu, Zhijun 1,2; Wang, Xinyan 1,2; Zhang, Xiaohui F.6,7; Tang, Chun 8; Wang, Hongda 5; Liu, Wanli 9; Ouyang, Bo 1,2; Wong, Catherine C.1,2; Cao, Yi 3,4; Xu, Chenqi1,2,10
	2017-04
发表期刊	CELL RESEARCH (IF:28.1[JCR-2023],36.4[5-Year])
ISSN	1001-0602
卷号	27 期号:4 页码:505-525
发表状态	已发表
DOI	10.1038/cr.2017.42
摘要	T-cell receptor-CD3 complex (TCR) is a versatile signaling machine that can initiate antigen-specific immune responses based on various biochemical changes of CD3 cytoplasmic domains, but the underlying structural basis remains elusive. Here we developed biophysical approaches to study the conformational dynamics of CD3 epsilon cytoplasmic domain (CD3 epsilon(CD)). At the single-molecule level, we found that CD3 epsilon(CD) could have multiple conformational states with different openness of three functional motifs, i.e., ITAM, BRS and PRS. These conformations were generated because different regions of CD3 epsilon(CD) had heterogeneous lipid-binding properties and therefore had heterogeneous dynamics. Live-cell imaging experiments demonstrated that different antigen stimulations could stabilize CD3 epsilon(CD) at different conformations. Lipid-dependent conformational dynamics thus provide structural basis for the versatile signaling property of TCR.
关键词	T-cell receptor lipid conformational dynamics atomic force microscopy nuclear magnetic resonance live-cell imaging
收录类别	SCI
语种	英语
资助项目	China Postdoctoral Science Foundation[2015M580357]
WOS研究方向	Cell Biology
WOS类目	Cell Biology
WOS记录号	WOS:000398770500007
出版者	INST BIOCHEMISTRY & CELL BIOLOGY
WOS关键词	ATOMIC-FORCE MICROSCOPY ; PARAMAGNETIC RELAXATION ENHANCEMENT ; SINGLE-MOLECULE EXPERIMENTS ; PROLINE-RICH SEQUENCE ; ANTIGEN RECEPTOR ; TYROSINE PHOSPHORYLATION ; MEMBRANE ASSOCIATION ; CYTOPLASMIC DOMAIN ; SUBUNIT CONTAINS ; FATE DECISIONS
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/1436
专题	生命科学与技术学院_特聘教授组_许琛琦组
通讯作者	Cao, Yi; Xu, Chenqi
作者单位	1.Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol, Shanghai Sci Res Ctr, CAS Ctr Excellence Mol Cell Sci,State Key Lab Mol, 320 Yueyang Rd, Shanghai 200031, Peoples R China 2.Univ Chinese Acad Sci, 320 Yueyang Rd, Shanghai 200031, Peoples R China 3.Nanjing Univ, Collaborat Innovat Ctr Adv Microstruct, Lab Solid State Microstruct, Nanjing 210093, Jiangsu, Peoples R China 4.Nanjing Univ, Dept Phys, Nanjing 210093, Jiangsu, Peoples R China 5.Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Elect Chem, Changchun 130022, Jilin, Peoples R China 6.Lehigh Univ, Bioengn Program, Bethlehem, PA 18015 USA 7.Lehigh Univ, Dept Mech Engn & Mech, Bethlehem, PA 18015 USA 8.Wuhan Inst Phys & Math, Chinese Acad Sci, State Key Lab Magnet Resonance & Atom Mol Phys, Wuhan 430071, Hubei, Peoples R China 9.Tsinghua Univ, Sch Life Sci, Beijing 100084, Peoples R China 10.ShanghaiTech Univ, Shanghai 201210, Peoples R China
通讯作者单位	上海科技大学
推荐引用方式 GB/T 7714	Guo, Xingdong,Yan, Chengsong,Li, Hua,et al. Lipid-dependent conformational dynamics underlie the functional versatility of T-cell receptor[J]. CELL RESEARCH,2017,27(4):505-525.
APA	Guo, Xingdong.,Yan, Chengsong.,Li, Hua.,Huang, Wenmao.,Shi, Xiaoshan.,...&Xu, Chenqi.(2017).Lipid-dependent conformational dynamics underlie the functional versatility of T-cell receptor.CELL RESEARCH,27(4),505-525.
MLA	Guo, Xingdong,et al."Lipid-dependent conformational dynamics underlie the functional versatility of T-cell receptor".CELL RESEARCH 27.4(2017):505-525.