Enhancing the precision of genetic lineage tracing using dual recombinases
He, Lingjuan1,2; Li, Yan1,2; Li, Yi1,2; Pu, Wenjuan1,2; Huang, Xiuzhen1,2; Tian, Xueying1,2; Wang, Yue1,2; Zhang, Hui1,2; Liu, Qiaozhen1,2; Zhang, Libo1,2; Zhao, Huan1,2; Tang, Juan1,2; Ji, Hongbin1,3; Cai, Dongqing4; Han, Zhibo5,6,7; Han, Zhongchao5,6,7; Nie, Yu7,8; Hu, Shengshou7,8; Wang, Qing-Dong9; Sun, Ruilin10; Fei, Jian10; Wang, Fengchao11; Chen, Ting11; Yan, Yan12; Huang, Hefeng13; Pu, William T.14,15; Zhou, Bin1,4,16
Source PublicationNATURE MEDICINE
AbstractThe Cre-loxP recombination system is the most widely used technology for in vivo tracing of stem or progenitor cell lineages. The precision of this genetic system largely depends on the specificity of Cre recombinase expression in targeted stem or progenitor cells. However, Cre expression in nontargeted cell types can complicate the interpretation of lineage-tracing studies and has caused controversy in many previous studies. Here we describe a new genetic lineage tracing system that incorporates the Dre-rox recombination system to enhance the precision of conventional Cre-loxP-mediated lineage tracing. The Dre-rox system permits rigorous control of Cre-loxP recombination in lineage tracing, effectively circumventing potential uncertainty of the cell-type specificity of Cre expression. Using this new system we investigated two topics of recent debates-the contribution of c-Kit(+) cardiac stem cells to cardiomyocytes in the heart and the contribution of Sox9(+) hepatic progenitor cells to hepatocytes in the liver. By overcoming the technical hurdle of nonspecific Cre-loxP-mediated recombination, this new technology provides more precise analysis of cell lineage and fate decisions and facilitates the in vivo study of stem and progenitor cell plasticity in disease and regeneration.
Indexed BySCI
Funding ProjectRising-Star Program[15QA1404300]
WOS Research AreaBiochemistry & Molecular Biology ; Cell Biology ; Research & Experimental Medicine
WOS SubjectBiochemistry & Molecular Biology ; Cell Biology ; Medicine, Research & Experimental
WOS IDWOS:000417278200019
Original Document TypeArticle
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Cited Times:46[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Corresponding AuthorZhou, Bin
Affiliation1.Univ Chinese Acad Sci, Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol, State Key Lab Cell Biol,CAS Ctr Excellence Mol C, Shanghai, Peoples R China
2.Univ Chinese Acad Sci, Chinese Acad Sci, Shanghai Inst Biol Sci, Key Lab Nutr & Metab,Inst Nutrit Sci, Shanghai, Peoples R China
3.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China
4.Jinan Univ, Inst Aging & Regenerat Med, Minist Educ, Key Lab Regenerat Med, Guangzhou, Guangdong, Peoples R China
5.Chinese Acad Med Sci, Inst Hematol, State Key Lab Expt Hematol, Tianjin, Peoples R China
6.Chinese Acad Med Sci, Hosp Blood Dis, Tianjin, Peoples R China
7.Peking Union Med Coll, Tianjin, Peoples R China
8.Chinese Acad Med Sci, Natl Ctr Cardiovasc Dis, Fuwai Hosp, State Key Lab Cardiovasc Dis, Beijing, Peoples R China
9.AstraZeneca, IMED Biotech Unit, Cardiovasc & Metab Dis, Biosci Heart Failure, Gothenburg, Sweden
10.Shanghai Model Organisms Ctr Inc, Shanghai, Peoples R China
11.Natl Inst Biol Sci, Beijing, Peoples R China
12.Fudan Univ, Zhongshan Hosp, Shanghai, Peoples R China
13.Shanghai Jiao Tong Univ, Sch Med, Int Peace Matern & Child Hlth Hosp, Shanghai, Peoples R China
14.Boston Childrens Hosp, Dept Cardiol, Boston, MA USA
15.Harvard Univ, Harvard Stem Cell Inst, Cambridge, MA 02138 USA
16.Tianjin Med Univ, Collaborat Innovat Ctr Tianjin Med Epigenet, Tianjin, Peoples R China
Recommended Citation
GB/T 7714
He, Lingjuan,Li, Yan,Li, Yi,et al. Enhancing the precision of genetic lineage tracing using dual recombinases[J]. NATURE MEDICINE,2017,23(12):1488-+.
APA He, Lingjuan.,Li, Yan.,Li, Yi.,Pu, Wenjuan.,Huang, Xiuzhen.,...&Zhou, Bin.(2017).Enhancing the precision of genetic lineage tracing using dual recombinases.NATURE MEDICINE,23(12),1488-+.
MLA He, Lingjuan,et al."Enhancing the precision of genetic lineage tracing using dual recombinases".NATURE MEDICINE 23.12(2017):1488-+.
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