Enhancing the precision of genetic lineage tracing using dual recombinases
He, Lingjuan1,2; Li, Yan1,2; Li, Yi1,2; Pu, Wenjuan1,2; Huang, Xiuzhen1,2; Tian, Xueying1,2; Wang, Yue1,2; Zhang, Hui1,2; Liu, Qiaozhen1,2; Zhang, Libo1,2
2017-12
发表期刊NATURE MEDICINE
ISSN1078-8956
卷号23期号:12页码:1488-+
发表状态已发表
DOI10.1038/nm.4437
摘要The Cre-loxP recombination system is the most widely used technology for in vivo tracing of stem or progenitor cell lineages. The precision of this genetic system largely depends on the specificity of Cre recombinase expression in targeted stem or progenitor cells. However, Cre expression in nontargeted cell types can complicate the interpretation of lineage-tracing studies and has caused controversy in many previous studies. Here we describe a new genetic lineage tracing system that incorporates the Dre-rox recombination system to enhance the precision of conventional Cre-loxP-mediated lineage tracing. The Dre-rox system permits rigorous control of Cre-loxP recombination in lineage tracing, effectively circumventing potential uncertainty of the cell-type specificity of Cre expression. Using this new system we investigated two topics of recent debates-the contribution of c-Kit(+) cardiac stem cells to cardiomyocytes in the heart and the contribution of Sox9(+) hepatic progenitor cells to hepatocytes in the liver. By overcoming the technical hurdle of nonspecific Cre-loxP-mediated recombination, this new technology provides more precise analysis of cell lineage and fate decisions and facilitates the in vivo study of stem and progenitor cell plasticity in disease and regeneration.
收录类别SCI
语种英语
资助项目Rising-Star Program[15QA1404300]
WOS研究方向Biochemistry & Molecular Biology ; Cell Biology ; Research & Experimental Medicine
WOS类目Biochemistry & Molecular Biology ; Cell Biology ; Medicine, Research & Experimental
WOS记录号WOS:000417278200019
出版者NATURE PUBLISHING GROUP
WOS关键词CARDIAC STEM-CELLS ; CRE RECOMBINASE ; C-KIT(+) CELLS ; PERIPORTAL HEPATOCYTES ; ENDOTHELIAL-CELLS ; DNA RECOMBINATION ; MAMMALIAN-CELLS ; DRE RECOMBINASE ; NEURAL CREST ; ADULT
原始文献类型Article
引用统计
文献类型期刊论文
条目标识符https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/14278
专题生命科学与技术学院_特聘教授组_季红斌组
生命科学与技术学院_特聘教授组_周斌组
通讯作者Zhou, Bin
作者单位1.Univ Chinese Acad Sci, Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol, State Key Lab Cell Biol,CAS Ctr Excellence Mol C, Shanghai, Peoples R China
2.Univ Chinese Acad Sci, Chinese Acad Sci, Shanghai Inst Biol Sci, Key Lab Nutr & Metab,Inst Nutrit Sci, Shanghai, Peoples R China
3.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China
4.Jinan Univ, Inst Aging & Regenerat Med, Minist Educ, Key Lab Regenerat Med, Guangzhou, Guangdong, Peoples R China
5.Chinese Acad Med Sci, Inst Hematol, State Key Lab Expt Hematol, Tianjin, Peoples R China
6.Chinese Acad Med Sci, Hosp Blood Dis, Tianjin, Peoples R China
7.Peking Union Med Coll, Tianjin, Peoples R China
8.Chinese Acad Med Sci, Natl Ctr Cardiovasc Dis, Fuwai Hosp, State Key Lab Cardiovasc Dis, Beijing, Peoples R China
9.AstraZeneca, IMED Biotech Unit, Cardiovasc & Metab Dis, Biosci Heart Failure, Gothenburg, Sweden
10.Shanghai Model Organisms Ctr Inc, Shanghai, Peoples R China
推荐引用方式
GB/T 7714
He, Lingjuan,Li, Yan,Li, Yi,et al. Enhancing the precision of genetic lineage tracing using dual recombinases[J]. NATURE MEDICINE,2017,23(12):1488-+.
APA He, Lingjuan.,Li, Yan.,Li, Yi.,Pu, Wenjuan.,Huang, Xiuzhen.,...&Zhou, Bin.(2017).Enhancing the precision of genetic lineage tracing using dual recombinases.NATURE MEDICINE,23(12),1488-+.
MLA He, Lingjuan,et al."Enhancing the precision of genetic lineage tracing using dual recombinases".NATURE MEDICINE 23.12(2017):1488-+.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
个性服务
查看访问统计
谷歌学术
谷歌学术中相似的文章
[He, Lingjuan]的文章
[Li, Yan]的文章
[Li, Yi]的文章
百度学术
百度学术中相似的文章
[He, Lingjuan]的文章
[Li, Yan]的文章
[Li, Yi]的文章
必应学术
必应学术中相似的文章
[He, Lingjuan]的文章
[Li, Yan]的文章
[Li, Yi]的文章
相关权益政策
暂无数据
收藏/分享
文件名: 14278.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。