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Enhancing the precision of genetic lineage tracing using dual recombinases | |
He, Lingjuan1,2; Li, Yan1,2; Li, Yi1,2; Pu, Wenjuan1,2; Huang, Xiuzhen1,2; Tian, Xueying1,2; Wang, Yue1,2; Zhang, Hui1,2; Liu, Qiaozhen1,2; Zhang, Libo1,2 | |
2017-12 | |
发表期刊 | NATURE MEDICINE |
ISSN | 1078-8956 |
卷号 | 23期号:12页码:1488-+ |
发表状态 | 已发表 |
DOI | 10.1038/nm.4437 |
摘要 | The Cre-loxP recombination system is the most widely used technology for in vivo tracing of stem or progenitor cell lineages. The precision of this genetic system largely depends on the specificity of Cre recombinase expression in targeted stem or progenitor cells. However, Cre expression in nontargeted cell types can complicate the interpretation of lineage-tracing studies and has caused controversy in many previous studies. Here we describe a new genetic lineage tracing system that incorporates the Dre-rox recombination system to enhance the precision of conventional Cre-loxP-mediated lineage tracing. The Dre-rox system permits rigorous control of Cre-loxP recombination in lineage tracing, effectively circumventing potential uncertainty of the cell-type specificity of Cre expression. Using this new system we investigated two topics of recent debates-the contribution of c-Kit(+) cardiac stem cells to cardiomyocytes in the heart and the contribution of Sox9(+) hepatic progenitor cells to hepatocytes in the liver. By overcoming the technical hurdle of nonspecific Cre-loxP-mediated recombination, this new technology provides more precise analysis of cell lineage and fate decisions and facilitates the in vivo study of stem and progenitor cell plasticity in disease and regeneration. |
收录类别 | SCI |
语种 | 英语 |
资助项目 | Rising-Star Program[15QA1404300] |
WOS研究方向 | Biochemistry & Molecular Biology ; Cell Biology ; Research & Experimental Medicine |
WOS类目 | Biochemistry & Molecular Biology ; Cell Biology ; Medicine, Research & Experimental |
WOS记录号 | WOS:000417278200019 |
出版者 | NATURE PUBLISHING GROUP |
WOS关键词 | CARDIAC STEM-CELLS ; CRE RECOMBINASE ; C-KIT(+) CELLS ; PERIPORTAL HEPATOCYTES ; ENDOTHELIAL-CELLS ; DNA RECOMBINATION ; MAMMALIAN-CELLS ; DRE RECOMBINASE ; NEURAL CREST ; ADULT |
原始文献类型 | Article |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/14278 |
专题 | 生命科学与技术学院_特聘教授组_季红斌组 生命科学与技术学院_特聘教授组_周斌组 |
通讯作者 | Zhou, Bin |
作者单位 | 1.Univ Chinese Acad Sci, Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol, State Key Lab Cell Biol,CAS Ctr Excellence Mol C, Shanghai, Peoples R China 2.Univ Chinese Acad Sci, Chinese Acad Sci, Shanghai Inst Biol Sci, Key Lab Nutr & Metab,Inst Nutrit Sci, Shanghai, Peoples R China 3.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China 4.Jinan Univ, Inst Aging & Regenerat Med, Minist Educ, Key Lab Regenerat Med, Guangzhou, Guangdong, Peoples R China 5.Chinese Acad Med Sci, Inst Hematol, State Key Lab Expt Hematol, Tianjin, Peoples R China 6.Chinese Acad Med Sci, Hosp Blood Dis, Tianjin, Peoples R China 7.Peking Union Med Coll, Tianjin, Peoples R China 8.Chinese Acad Med Sci, Natl Ctr Cardiovasc Dis, Fuwai Hosp, State Key Lab Cardiovasc Dis, Beijing, Peoples R China 9.AstraZeneca, IMED Biotech Unit, Cardiovasc & Metab Dis, Biosci Heart Failure, Gothenburg, Sweden 10.Shanghai Model Organisms Ctr Inc, Shanghai, Peoples R China |
推荐引用方式 GB/T 7714 | He, Lingjuan,Li, Yan,Li, Yi,et al. Enhancing the precision of genetic lineage tracing using dual recombinases[J]. NATURE MEDICINE,2017,23(12):1488-+. |
APA | He, Lingjuan.,Li, Yan.,Li, Yi.,Pu, Wenjuan.,Huang, Xiuzhen.,...&Zhou, Bin.(2017).Enhancing the precision of genetic lineage tracing using dual recombinases.NATURE MEDICINE,23(12),1488-+. |
MLA | He, Lingjuan,et al."Enhancing the precision of genetic lineage tracing using dual recombinases".NATURE MEDICINE 23.12(2017):1488-+. |
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