Structural and mechanistic insights into regulation of HBO1 histone acetyltransferase activity by BRPF2

doi:10.1093/nar/gkx142

	Structural and mechanistic insights into regulation of HBO1 histone acetyltransferase activity by BRPF2
	Tao, Ye 1,2; Zhong, Chen 1,2; Zhu, Junjun 1,2; Xu, Shutong 1,2; Ding, Jianping1,2,3,4,5
	2017-06-02
发表期刊	NUCLEIC ACIDS RESEARCH (IF:16.6[JCR-2023],16.1[5-Year])
ISSN	0305-1048
卷号	45 期号:10 页码:5707-5719
发表状态	已发表
DOI	10.1093/nar/gkx142
摘要	HBO1, a member of the MYST family of histone acetyltransferases (HATs), is required for global acetylation of histone H3K14 and embryonic development. It functions as a catalytic subunit in multisubunit complexes comprising a BRPF1/2/3 or JADE1/2/3 scaffold protein, and two accessory proteins. BRPF2 has been shown to be important for the HAT activity of HBO1 toward H3K14. Here we demonstrated that BRPF2 can regulate the HAT activity of HBO1 toward free H3 and H4, and nucleosomal H3. Particularly, a short N-terminal region of BRPF2 is sufficient for binding to HBO1 and can potentiate its activity toward H3K14. The crystal structure of the HBO1 MYST domain in complex with this segment of BRPF2 together with the biochemical and cell biological data revealed the key residues responsible for the HBO1-BRPF2 interaction. Our structural and functional data together indicate that the N-terminal region of BRPF2 plays an important role in the binding of HBO1 and a minor role in the binding of nucleosomes, which provide new mechanistic insights into the regulation of the HAT activity of HBO1 by BRPF2.
收录类别	SCI
语种	英语
资助项目	Ministry of Science and Technology of China[2013CB910404]
WOS研究方向	Biochemistry & Molecular Biology
WOS类目	Biochemistry & Molecular Biology
WOS记录号	WOS:000402510700024
出版者	OXFORD UNIV PRESS
WOS关键词	PHD FINGER ; H3 TAIL ; MOLECULAR-BASIS ; HAT COMPLEXES ; ACETYLATION ; CHROMATIN ; RECOGNITION ; DOMAIN ; NUA4 ; DNA
原始文献类型	Article
通讯作者	Ding, Jianping
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/1340
专题	生命科学与技术学院_特聘教授组_丁建平组
通讯作者	Ding, Jianping
作者单位	1.Chinese Acad Sci, Ctr Excellence Mol Cell Sci, Natl Ctr Prot Sci Shanghai, Shanghai Inst Biochem & Cell Biol,State Key Lab M, 320 Yue Yang Rd, Shanghai 200031, Peoples R China 2.Univ Chinese Acad Sci, Chinese Acad Sci, 320 Yue Yang Rd, Shanghai 200031, Peoples R China 3.Shanghai Tech Univ, Sch Life Sci & Technol, 100 Haike Rd, Shanghai 201210, Peoples R China 4.Chinese Acad Sci, Shanghai Sci Res Ctr, 333 Haike Rd, Shanghai 201210, Peoples R China 5.Fudan Univ, Collaborat Innovat Ctr Genet & Dev, 2005 Songhu Rd, Shanghai 200438, Peoples R China
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Tao, Ye,Zhong, Chen,Zhu, Junjun,et al. Structural and mechanistic insights into regulation of HBO1 histone acetyltransferase activity by BRPF2[J]. NUCLEIC ACIDS RESEARCH,2017,45(10):5707-5719.
APA	Tao, Ye,Zhong, Chen,Zhu, Junjun,Xu, Shutong,&Ding, Jianping.(2017).Structural and mechanistic insights into regulation of HBO1 histone acetyltransferase activity by BRPF2.NUCLEIC ACIDS RESEARCH,45(10),5707-5719.
MLA	Tao, Ye,et al."Structural and mechanistic insights into regulation of HBO1 histone acetyltransferase activity by BRPF2".NUCLEIC ACIDS RESEARCH 45.10(2017):5707-5719.