SARS-CoV-2 infection and replication in human gastric organoids

doi:10.1038/s41467-021-26762-2

KMS > 免疫化学研究所 > 特聘教授组 > Nicola Elvassore组

	SARS-CoV-2 infection and replication in human gastric organoids
	Giobbe, Giovanni Giuseppe 1; Bonfante, Francesco 2; Jones, Brendan C.1; Gagliano, Onelia 3; Luni, Camilla4 ; Zambaiti, Elisa 1,3,5; Perin, Silvia 1; Laterza, Cecilia 3; Busslinger, Georg 6,7; Stuart, Hannah 3; Pagliari, Matteo 2; Bortolami, Alessio 2; Mazzetto, Eva 2; Manfredi, Anna 8,9; Colantuono, Chiara 8,9; Di Filippo, Lucio 8,9; Pellegata, Alessandro Filippo 1; Panzarin, Valentina 2; Thapar, Nikhil 10; Li, Vivian Sze Wing 11; Eaton, Simon 1; Cacchiarelli, Davide 8,12; Clevers, Hans 6,7,13; Elvassore, Nicola1,3,4,14 ; De Coppi, Paolo 1,15
	2021
发表期刊	NATURE COMMUNICATIONS (IF:14.7[JCR-2023],16.1[5-Year])
EISSN	2041-1723
卷号	12 期号:1
DOI	10.1038/s41467-021-26762-2
摘要	COVID-19 typically manifests as a respiratory illness, but several clinical reports have described gastrointestinal symptoms. This is particularly true in children in whom gastrointestinal symptoms are frequent and viral shedding outlasts viral clearance from the respiratory system. These observations raise the question of whether the virus can replicate within the stomach. Here we generate gastric organoids from fetal, pediatric, and adult biopsies as in vitro models of SARS-CoV-2 infection. To facilitate infection, we induce reverse polarity in the gastric organoids. We find that the pediatric and late fetal gastric organoids are susceptible to infection with SARS-CoV-2, while viral replication is significantly lower in undifferentiated organoids of early fetal and adult origin. We demonstrate that adult gastric organoids are more susceptible to infection following differentiation. We perform transcriptomic analysis to reveal a moderate innate antiviral response and a lack of differentially expressed genes belonging to the interferon family. Collectively, we show that the virus can efficiently infect the gastric epithelium, suggesting that the stomach might have an active role in fecal-oral SARS-CoV-2 transmission. Several clinical reports have described gastrointestinal symptoms for COVID-19, though whether the virus can replicate within the stomach remains unclear. Here the authors generate gastric organoids from human biopsies and show that the virus can efficiently infect gastric epithelium, suggesting that the stomach might have an active role in fecal-oral transmission.
收录类别	SCIE
语种	英语
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000719546000024
出版者	NATURE PORTFOLIO
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/133150
专题	免疫化学研究所_特聘教授组_生物工程学实验室
通讯作者	Giobbe, Giovanni Giuseppe; Elvassore, Nicola; De Coppi, Paolo
作者单位	1.UCL, GOS Inst Child Hlth, Stem Cell & Regenerat Med Sect, London, England; 2.Ist Zooprofilatt Sperimentale Venezie, Div Comparat Biomed Sci, Lab Expt Anim Models, Legnaro, Italy; 3.Veneto Inst Mol Med VIMM, Padua, Italy; 4.ShanghaiTech Univ, Shanghai Inst Adv Immunochem Studies SIAIS, Shanghai, Peoples R China; 5.Univ Padua, Dept Womens & Childrens Hlth, Padua, Italy; 6.Royal Netherlands Acad Arts & Sci KNAW, Hubrecht Inst, Oncode Inst, Utrecht, Netherlands; 7.Univ Med Ctr UMC, Utrecht, Netherlands; 8.Armenise Harvard Lab Integrat Genom, Telethon Inst Genet & Med TIGEM, Pozzuoli, Italy; 9.Next Generat Diagnost Srl, Pozzuoli, Italy; 10.Queensland Childrens Hosp, Gastroenterol Hepatol & Liver Transplant, Brisbane, Qld, Australia; 11.Francis Crick Inst, Stem Cell & Canc Biol Lab, London, England; 12.Univ Naples Federico II, Dept Translat Med, Naples, Italy; 13.Princess Maxima Ctr PMC Pediat Oncol, Utrecht, Netherlands; 14.Univ Padua, Dept Ind Engn, Padua, Italy; 15.Great Ormond St Hosp Sick Children, Dept Specialist Neonatal & Paediat Surg, London, England
通讯作者单位	免疫化学研究所
推荐引用方式 GB/T 7714	Giobbe, Giovanni Giuseppe,Bonfante, Francesco,Jones, Brendan C.,et al. SARS-CoV-2 infection and replication in human gastric organoids[J]. NATURE COMMUNICATIONS,2021,12(1).
APA	Giobbe, Giovanni Giuseppe.,Bonfante, Francesco.,Jones, Brendan C..,Gagliano, Onelia.,Luni, Camilla.,...&De Coppi, Paolo.(2021).SARS-CoV-2 infection and replication in human gastric organoids.NATURE COMMUNICATIONS,12(1).
MLA	Giobbe, Giovanni Giuseppe,et al."SARS-CoV-2 infection and replication in human gastric organoids".NATURE COMMUNICATIONS 12.1(2021).