上海科技大学知识管理系统

本发明公开了一种生物制品中虹鳟源性成分实时荧光PCR检测方法，包括以下步骤：第一步，以待测样品的DNA为模板，进行荧光定量PCR扩增，获得PCR扩增产物；第二步，检测扩增产物的荧光信号；第三步，以检测结果的Ct值判定样品中是否含有虹鳟源性成分；其中，用于PCR扩增的反应体系中含有扩增虹鳟源性成分的特异性引物对和虹鳟源性成分的特异性探针。本发明的虹鳟源性成分的实时荧光PCR扩增的特异性引物对和探针不仅特异性好，而且灵敏度高，为快速准确地检测动物制品中是否含有虹鳟源性成分提供了一种定性检测方法，具有较好的应用前景。

The invention discloses a real-time fluorescent PCR detection method for rainbow trout-derived components in a biological product. The real-time fluorescent PCR detection method comprises the following steps of 1, performing fluorescent quantitative PCR amplification by taking DNA of a sample to be detected as a template to obtain a PCR amplification product; 2, detecting a fluorescence signal of the amplification product; and 3, judging whether the sample contains the rainbow trout-derived components or not according to a Ct value of a detection result, wherein a reaction system for the PCR amplification contains a specific primer for amplifying the rainbow trout-derived components and a specific probe for amplifying the rainbow trout-derived components. The specific primer and the probe for real-time fluorescence PCR amplification of the rainbow trout-derived components are good in specificity and high in sensitivity, the qualitative detection method is provided for rapidly and accurately detecting whether animal products contain the rainbow trout-derived components or not, and the real-time fluorescent PCR detection method has good application prospects.