上海科技大学知识管理系统

本发明公开了一种基于CRISPR荧光法检测大西洋鲑核酸的检测试剂盒，包括适用于大西洋鲑快速核酸检测的CRISPR的荧光检测体系，所述CRISPR的荧光检测体系包括：针对大西洋鲑线粒体靶序列的特异性crRNA、高效等温扩增引物组合、CRISPR蛋白和ssDNA报告系统。本发明还公开一种基于CRISPR荧光法检测大西洋鲑核酸的快速检测方法，其是采用所述的基于CRISPR荧光法检测大西洋鲑核酸的检测试剂盒进行检测。本发明的检测试剂盒，既可利用酶标仪荧光检测，也可以利用荧光肉眼检测，具有良好的灵敏性和特异性。

The invention discloses a detection kit for detecting nucleic acid of Atlantic salmon based on a CRISPR fluorescence method. The detection kit comprises a CRISPR fluorescence detection system suitable for rapid nucleic acid detection of Atlantic salmon, wherein the CRISPR fluorescence detection system comprises specific crRNA aiming at a mitochondrial target sequence of Atlantic salmon, an efficient isothermal amplification primer combination, CRISPR protein and an ssDNA report system. The invention further discloses a rapid detection method for detecting the nucleic acid of the Atlantic salmon based on the CRISPR fluorescence method, and the detection kit for detecting the nucleic acid of the Atlantic salmon based on the CRISPR fluorescence method is adopted for detection. The detection kit disclosed by the invention not only can be used for fluorescence detection by using a microplate reader, but also can be used for naked eye detection by using fluorescence, and has good sensitivity and specificity.