PUPIL enables mapping and stamping of transient electrical connectivity in developing nervous systems

doi:10.1016/j.celrep.2021.109853

	PUPIL enables mapping and stamping of transient electrical connectivity in developing nervous systems
	Xie, Shu1,2,3 ; Li, Haixiang1,2,3 ; Yao, Fenyong1 ; Huang, Jiechang1,2,3 ; Yang, Xiaomei1 ; Chen, Xin1,2,3 ; Liu, Qiang1,2,3 ; Zhuang, Min1 ; He, Shuijin1
	2021-10-19
发表期刊	CELL REPORTS (IF:7.5[JCR-2023],8.5[5-Year])
ISSN	2211-1247
卷号	37 期号:3
DOI	10.1016/j.celrep.2021.109853
摘要	Currently, many genetic methods are available for mapping chemical connectivity, but analogous methods for electrical synapses are lacking. Here, we present pupylation-based interaction labeling (PUPIL), a genetically encoded system for noninvasively mapping and stamping transient electrical synapses in the mouse brain. Upon fusion of connexin 26 (CX26) with the ligase PafA, pupylation yields tag puncta following conjugation of its substrate, a biotin- or fluorescent-protein-tagged PupE, to the neighboring proteins of electrical synapses containing CX26-PafA. Tag puncta are validated to correlate well with functional electrical synapses in immature neurons. Furthermore, puncta are retained in mature neurons when electrical synapses mostly disappear-suggesting successful stamping. We use PUPIL to uncover spatial subcellular localizations of electrical synapses and approach their physiological functions during development. Thus, PUPIL is a powerful tool for probing electrical connectivity patterns in complex nervous systems and has great potential for transient receptors and ion channels as well.
收录类别	SCIE
语种	英语
WOS研究方向	Cell Biology
WOS类目	Cell Biology
WOS记录号	WOS:000709243200021
出版者	CELL PRESS
原始文献类型	Article
引用统计	被引频次：7[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/131820
专题	生命科学与技术学院_博士生生命科学与技术学院_PI研究组_庄敏组生命科学与技术学院_PI研究组_何水金组生命科学与技术学院_硕士生
通讯作者	He, Shuijin
作者单位	1.ShanghaiTech Univ, Sch Life Sci & Technol, 393 Middle Huaxia Rd, Shanghai 201210, Peoples R China; 2.Chinese Acad Sci, CAS Ctr Excellence Brain Sci & Intelligence Techn, Inst Neurosci, State Key Lab Neurosci, Shanghai 200031, Peoples R China; 3.Univ Chinese Acad Sci, Beijing, Peoples R China
第一作者单位	生命科学与技术学院
通讯作者单位	生命科学与技术学院
第一作者的第一单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Xie, Shu,Li, Haixiang,Yao, Fenyong,et al. PUPIL enables mapping and stamping of transient electrical connectivity in developing nervous systems[J]. CELL REPORTS,2021,37(3).
APA	Xie, Shu.,Li, Haixiang.,Yao, Fenyong.,Huang, Jiechang.,Yang, Xiaomei.,...&He, Shuijin.(2021).PUPIL enables mapping and stamping of transient electrical connectivity in developing nervous systems.CELL REPORTS,37(3).
MLA	Xie, Shu,et al."PUPIL enables mapping and stamping of transient electrical connectivity in developing nervous systems".CELL REPORTS 37.3(2021).