Exploration of alcohol use disorder-associated brain miRNA-mRNA regulatory networks

doi:10.1038/s41398-021-01635-w

	Exploration of alcohol use disorder-associated brain miRNA-mRNA regulatory networks
	Lim, Yolpanhchana 1,2; Beane-Ebel, Jennifer E.3; Tanaka, Yoshiaki 4,10; Ning, Boting 3; Husted, Christopher R.3; Henderson, David C.1; Xiang, Yangfei4,11 ; Park, In-Hyun 4; Farrer, Lindsay A.5,6,7,8,9; Zhang, Huiping 1,8,9
	2021-10-02
发表期刊	TRANSLATIONAL PSYCHIATRY (IF:5.8[JCR-2023],6.3[5-Year])
ISSN	2158-3188
卷号	11 期号:1
DOI	10.1038/s41398-021-01635-w
摘要	Transcriptomic changes in specific brain regions can influence the risk of alcohol use disorder (AUD), but the underlying mechanism is not fully understood. We investigated AUD-associated miRNA-mRNA regulatory networks in multiple brain regions by analyzing transcriptomic changes in two sets of postmortem brain tissue samples and ethanol-exposed human embryonic stem cell (hESC)-derived cortical interneurons. miRNA and mRNA transcriptomes were profiled in 192 tissue samples (Set 1) from eight brain regions (amygdala, caudate nucleus, cerebellum, hippocampus, nucleus accumbens, prefrontal cortex, putamen, and ventral tegmental area) of 12 AUD and 12 control European Australians. Nineteen differentially expressed miRNAs (fold-change>2.0 & P < 0.05) and 97 differentially expressed mRNAs (fold-change>2.0 & P < 0.001) were identified in one or multiple brain regions of AUD subjects. AUD-associated miRNA-mRNA regulatory networks in each brain region were constructed using differentially expressed and negatively correlated miRNA-mRNA pairs. AUD-relevant pathways (including CREB Signaling, IL-8 Signaling, and Axonal Guidance Signaling) were potentially regulated by AUD-associated brain miRNA-mRNA pairs. Moreover, miRNA and mRNA transcriptomes were mapped in additional 96 tissue samples (Set 2) from six of the above eight brain regions of eight AUD and eight control European Australians. Some of the AUD-associated miRNA-mRNA regulatory networks were confirmed. In addition, miRNA and mRNA transcriptomes were analyzed in hESC-derived cortical interneurons with or without ethanol exposure, and ethanol-influenced miRNA-mRNA regulatory networks were constructed. This study provided evidence that alcohol could induce concerted miRNA and mRNA expression changes in reward-related or alcohol-responsive brain regions. We concluded that altered brain miRNA-mRNA regulatory networks might contribute to AUD development.
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收录类别	SCIE
语种	英语
WOS研究方向	Psychiatry
WOS类目	Psychiatry
WOS记录号	WOS:000702784100002
出版者	SPRINGERNATURE
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/128300
专题	生命科学与技术学院_PI研究组_向阳飞组
通讯作者	Zhang, Huiping
作者单位	1.Boston Univ, Sch Med, Dept Psychiat, Boston, MA 02118 USA; 2.Boston Univ, Grad Sch Arts & Sci, Bioinformat Program, Boston, MA 02215 USA; 3.Boston Univ, Sch Med, Dept Med, Sect Computat Biomed, Boston, MA 02118 USA; 4.Yale Univ, Sch Med, Dept Genet, New Haven, CT 06510 USA; 5.Boston Univ, Sch Med, Dept Med, Sect Biomed Genet, Boston, MA 02118 USA; 6.Boston Univ, Sch Med, Dept Neurol, Boston, MA 02118 USA; 7.Boston Univ, Sch Med, Dept Ophthalmol, Boston, MA 02118 USA; 8.Boston Univ, Sch Publ Hlth, Dept Biostat, Boston, MA 02118 USA; 9.Boston Univ, Sch Publ Hlth, Dept Epidemiol, Boston, MA 02118 USA; 10.Univ Montreal, Maisonneuve Rosemt Hosp Res Ctr, Dept Med, Montreal, PQ H1T 2M4, Canada; 11.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China
推荐引用方式 GB/T 7714	Lim, Yolpanhchana,Beane-Ebel, Jennifer E.,Tanaka, Yoshiaki,et al. Exploration of alcohol use disorder-associated brain miRNA-mRNA regulatory networks[J]. TRANSLATIONAL PSYCHIATRY,2021,11(1).
APA	Lim, Yolpanhchana.,Beane-Ebel, Jennifer E..,Tanaka, Yoshiaki.,Ning, Boting.,Husted, Christopher R..,...&Zhang, Huiping.(2021).Exploration of alcohol use disorder-associated brain miRNA-mRNA regulatory networks.TRANSLATIONAL PSYCHIATRY,11(1).
MLA	Lim, Yolpanhchana,et al."Exploration of alcohol use disorder-associated brain miRNA-mRNA regulatory networks".TRANSLATIONAL PSYCHIATRY 11.1(2021).