Multiregional profiling of the brain transmembrane proteome uncovers novel regulators of depression
Li, Shanshan1; Luo, Huoqing2,3; Lou, Ronghui1,2,3; Tian, Cuiping1; Miao, Chen1; Xia, Lisha1,2,3; Pan, Chen1; Duan, Xiaoxiao1; Dang, Ting1,2,3; Li, Hui1; Fan, Chengyu2; Tang, Pan1,2,3; Zhang, Zhuangzhuang1,2,3; Liu, Yan1; Li, Yunxia4; Xu, Fei1,2; Zhang, Yaoyang4; Zhong, Guisheng1,2; Hu, Ji2,5; Shui, Wenqing1,2
2021-07-21
Source PublicationSCIENCE ADVANCES
ISSN2375-2548
Volume7Issue:30
Status已发表
DOI10.1126/sciadv.abf0634
Abstract

Transmembrane proteins play vital roles in mediating synaptic transmission, plasticity, and homeostasis in the brain. However, these proteins, especially the G protein–coupled receptors (GPCRs), are underrepresented in most large-scale proteomic surveys. Here, we present a new proteomic approach aided by deep learning models for comprehensive profiling of transmembrane protein families in multiple mouse brain regions. Our multiregional proteome profiling highlights the considerable discrepancy between messenger RNA and protein distribution, especially for region-enriched GPCRs, and predicts an endogenous GPCR interaction network in the brain. Furthermore, our new approach reveals the transmembrane proteome remodeling landscape in the brain of a mouse depression model, which led to the identification of two previously unknown GPCR regulators of depressive-like behaviors. Our study provides an enabling technology and rich data resource to expand the understanding of transmembrane proteome organization and dynamics in the brain and accelerate the discovery of potential therapeutic targets for depression treatment.

KeywordGPCR Membrane proteomics DIA proteomics Depression Mouse depression model Animal behavioral test
MOST Discipline Catalogue授予和人才培养学科目录::理学::生物学
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Indexed BySCI
Language英语
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Cited Times [WOS]:0   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttps://kms.shanghaitech.edu.cn/handle/2MSLDSTB/128266
CollectioniHuman研究所_PI研究组_水雯箐组
生命科学与技术学院_PI研究组_胡霁组
iHuman研究所_特聘教授组_Raymond Stevens组
iHuman研究所_公共科研平台_超分辨生物成像平台
生命科学与技术学院_公共科研平台_分子影像平台
iHuman研究所_PI研究组_徐菲组
iHuman研究所_PI研究组_钟桂生组
iHuman研究所_PI研究组_Garth John Thompson组
生命科学与技术学院_硕士生
生命科学与技术学院_博士生
Co-First AuthorLuo, Huoqing; Lou, Ronghui; Tian, Cuiping
Corresponding AuthorZhong, Guisheng; Hu, Ji; Shui, Wenqing
Affiliation1.iHuman Institute, ShanghaiTech University, Shanghai 201210, China
2.CAS Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai 200031, China
3.School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China
4.University of Chinese Academy of Sciences, Beijing 100049, China
5.Interdisciplinary Research Center on Biology and Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 201210, China
First Author AffilicationiHuman Institute
Corresponding Author AffilicationiHuman Institute
First Signature AffilicationiHuman Institute
Recommended Citation
GB/T 7714
Li, Shanshan,Luo, Huoqing,Lou, Ronghui,et al. Multiregional profiling of the brain transmembrane proteome uncovers novel regulators of depression[J]. SCIENCE ADVANCES,2021,7(30).
APA Li, Shanshan.,Luo, Huoqing.,Lou, Ronghui.,Tian, Cuiping.,Miao, Chen.,...&Shui, Wenqing.(2021).Multiregional profiling of the brain transmembrane proteome uncovers novel regulators of depression.SCIENCE ADVANCES,7(30).
MLA Li, Shanshan,et al."Multiregional profiling of the brain transmembrane proteome uncovers novel regulators of depression".SCIENCE ADVANCES 7.30(2021).
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