上海科技大学知识管理系统

本发明涉及动物基因工程和基因遗传修饰技术领域，特别涉及一种CRBN基因人源化动物肿瘤细胞模型的构建方法和用途。一种CRBN基因人源化动物肿瘤细胞模型的构建方法，所述构建方法包括：将肿瘤细胞引入人源化小鼠中，所述人源化小鼠的CRBN基因包括I391V突变。本发明构建的CRBN基因人源化动物肿瘤细胞模型不仅可用于免疫调节药物药效的评估，对药物开发的临床效果也具有重要的指导意义。同时也是IMiDs类药物安全性评价的理想小鼠肿瘤细胞模型，可对IMiDs类药物的毒理学进行临床前体评价，填补了市场空白。

The invention relates to the technical field of animal gene engineering and genetic modification. The invention relates to CRBN gene humanized animal tumor cell model construction method and application. The method for constructing CRBN gene humanized animal tumor cell model comprises the following steps : introducing tumor cells into the humanized mouse; CRBN gene of the humanized mouse comprises I391V mutation. CRBN Gene humanized animal tumor cell model constructed by the invention not only can be used for evaluating the drug effect of immunomodulatory drugs, but also has important guiding significance for the clinical effect of drug development. , The invention also provides an ideal mouse tumor cell model for IMiDs class of drug safety evaluation. The toxicology of IMiDs drugs can be evaluated clinically, and the market blank is filled up.