SUMOylation of alpha-tubulin is a novel modification regulating microtubule dynamics

doi:10.1093/jmcb/mjaa076

	SUMOylation of alpha-tubulin is a novel modification regulating microtubule dynamics
	Feng, Wenfeng 1,2,3; Liu, Rong 1; Xie, Xuan 1; Diao, Lei 1; Gao, Nannan 1; Cheng, Jinke 4,5; Zhang, Xu2,3,6,7,8 ; Li, Yong 4,5; Bao, Lan1,8
	2021-02
发表期刊	JOURNAL OF MOLECULAR CELL BIOLOGY (IF:5.3[JCR-2023],6.1[5-Year])
ISSN	1674-2788
EISSN	1759-4685
卷号	13 期号:2 页码:91-103
DOI	10.1093/jmcb/mjaa076
摘要	Microtubules (MTs) are regulated by a number of known posttranslational modifications (PTMs) on alpha/beta-tubulin to fulfill diverse cellular functions. Here, we showed that SUMOylation is a novel PTM on alpha-tubulin in vivo and in vitro. The SUMOylation on alpha-tubulin mainly occurred at Lys 96 (K96), K166, and K304 of soluble alpha-tubulin and could be removed by small ubiquitin-related modifier (SUMO)-specific peptidase 1. In vitro experiments showed that tubulin SUMOylation could reduce interprotofilament interaction, promote MT catastrophe, and impede MT polymerization. In cells, mutation of the SUMOylation sites on alpha-tubulin reduced catastrophe frequency and increased the proportion of polymerized alpha-tubulin, while upregulation of SUMOylation with fusion of SUMO1 reduced alpha-tubulin assembly into MTs. Additionally, overexpression of SUMOylation-deficient alpha-tubulin attenuated the neurite extension in Neuro-2a cells. Thus, SUMOylation on alpha-tubulin represents a new player in the regulation of MT properties.
关键词	alpha-tubulin SUMOylation microtubule dynamics microtubule assembly
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收录类别	SCIE
语种	英语
WOS研究方向	Cell Biology
WOS类目	Cell Biology
WOS记录号	WOS:000654661700002
出版者	OXFORD UNIV PRESS
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/126951
专题	生命科学与技术学院_特聘教授组_张旭组生命科学与技术学院_特聘教授组_鲍岚组
通讯作者	Bao, Lan
作者单位	1.Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol, CAS Ctr Excellence Mol Cell Sci, Univ Chinese Acad Sci,State Key Lab Cell Biol, Shanghai 200031, Peoples R China; 2.Inst Brain Intelligence Technol, Zhangjiang Lab, Shanghai 201210, Peoples R China; 3.Shanghai Res Ctr Brain Sci & Brain Inspired Intel, Shanghai 201210, Peoples R China; 4.Shanghai Jiao Tong Univ, Sch Med, Collaborat Innovat Ctr Brain Sci, Discipline Neurosci, Shanghai 200025, Peoples R China; 5.Shanghai Jiao Tong Univ, Sch Med, Collaborat Innovat Ctr Brain Sci, Dept Biochem, Shanghai 200025, Peoples R China; 6.Chinese Acad Sci, CAS Ctr Excellence Brain Sci, Inst Neurosci, Shanghai 200031, Peoples R China; 7.Chinese Acad Sci, CAS Ctr Excellence Brain Sci, State Key Lab Neurosci, Shanghai 200031, Peoples R China; 8.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Feng, Wenfeng,Liu, Rong,Xie, Xuan,et al. SUMOylation of alpha-tubulin is a novel modification regulating microtubule dynamics[J]. JOURNAL OF MOLECULAR CELL BIOLOGY,2021,13(2):91-103.
APA	Feng, Wenfeng.,Liu, Rong.,Xie, Xuan.,Diao, Lei.,Gao, Nannan.,...&Bao, Lan.(2021).SUMOylation of alpha-tubulin is a novel modification regulating microtubule dynamics.JOURNAL OF MOLECULAR CELL BIOLOGY,13(2),91-103.
MLA	Feng, Wenfeng,et al."SUMOylation of alpha-tubulin is a novel modification regulating microtubule dynamics".JOURNAL OF MOLECULAR CELL BIOLOGY 13.2(2021):91-103.