A widespread pathway for substitution of adenine by diaminopurine in phage genomes

doi:10.1126/science.abe4882

	A widespread pathway for substitution of adenine by diaminopurine in phage genomes
	Zhou, Yan 1,2; Xu, Xuexia3,4,5,6 ; Wei, Yifeng 7; Cheng, Yu3,4 ; Guo, Yu3,4 ; Khudyakov, Ivan 8; Liu, Fuli 9; He, Ping 9; Song, Zhangyue10 ; Li, Zhi 1; Gao, Yan 1; Ang, Ee Lui 7; Zhao, Huimin 7,11; Zhang, Yan 1,2; Zhao, Suwen3,4
	2021
发表期刊	SCIENCE (IF:44.7[JCR-2023],50.3[5-Year])
ISSN	0036-8075
EISSN	1095-9203
卷号	372 期号:6541 页码:512-+
发表状态	已发表
DOI	10.1126/science.abe4882
摘要	DNA modifications vary in form and function but generally do not alter Watson-Crick base pairing. Diaminopurine (Z) is an exception because it completely replaces adenine and forms three hydrogen bonds with thymine in cyanophage S-2L genomic DNA. However, the biosynthesis, prevalence, and importance of Z genomes remain unexplored. Here, we report a multienzyme system that supports Z-genome synthesis. We identified dozens of globally widespread phages harboring such enzymes, and we further verified the Z genome in one of these phages, Acinetobacter phage SH-Ab 15497, by using liquid chromatography with ultraviolet and mass spectrometry. The Z genome endows phages with evolutionary advantages for evading the attack of host restriction enzymes, and the characterization of its biosynthetic pathway enables Z-DNA production on a large scale for a diverse range of applications.
收录类别	SCIE
语种	英语
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000645554100047
出版者	AMER ASSOC ADVANCEMENT SCIENCE
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/126807
专题	生命科学与技术学院_博士生 iHuman研究所_PI研究组_赵素文组生命科学与技术学院_硕士生免疫化学研究所_公共科研平台_生物医学大数据平台
通讯作者	Zhao, Huimin; Zhang, Yan; Zhao, Suwen
作者单位	1.Tianjin Univ, Tianjin Key Lab Modem Drug Delivery & High Effici, Collaborat Innovat Ctr Chem Sci & Engn, Sch Pharmaceut Sci & Technol, Tianjin 300072, Peoples R China; 2.Tianjin Univ, Frontiers Sci Ctr Synthet Biol, Minist Educ, Tianjin 300072, Peoples R China; 3.Shanghai Tech Univ, iHuman Inst, Shanghai 201210, Peoples R China; 4.Shanghai Tech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China; 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China; 6.Chinese Acad Sci, Shanghai Inst Nutr & Hlth, Shanghai 200061, Peoples R China; 7.ASTAR, Singapore Inst Food & Biotechnol Innovat, Singapore, Singapore; 8.All Russian Res Inst Agr Microbiol, St Petersburg 196608, Russia; 9.Shanghai Jiao Tong Univ, Dept Med Microbiol & Immunol, Sch Med, Shanghai 200025, Peoples R China; 10.Shanghai Tech Univ, SIAIS, Biomed Big Data Platform, Shanghai 201210, Peoples R China; 11.Univ Illinois, Dept Chem & Biomol Engn, Urbana, IL 61801 USA
通讯作者单位	上海科技大学; 生命科学与技术学院
推荐引用方式 GB/T 7714	Zhou, Yan,Xu, Xuexia,Wei, Yifeng,et al. A widespread pathway for substitution of adenine by diaminopurine in phage genomes[J]. SCIENCE,2021,372(6541):512-+.
APA	Zhou, Yan.,Xu, Xuexia.,Wei, Yifeng.,Cheng, Yu.,Guo, Yu.,...&Zhao, Suwen.(2021).A widespread pathway for substitution of adenine by diaminopurine in phage genomes.SCIENCE,372(6541),512-+.
MLA	Zhou, Yan,et al."A widespread pathway for substitution of adenine by diaminopurine in phage genomes".SCIENCE 372.6541(2021):512-+.