Dual recombinases-based genetic lineage tracing for stem cell research with enhanced precision

doi:10.1007/s11427-020-1889-9

	Dual recombinases-based genetic lineage tracing for stem cell research with enhanced precision
	Jin, Hengwei 1; Liu, Kuo 1,3; Zhou, Bin1,2,3
	2021-12
发表期刊	SCIENCE CHINA-LIFE SCIENCES (IF:8.0[JCR-2023],7.3[5-Year])
ISSN	1674-7305
EISSN	1869-1889
DOI	10.1007/s11427-020-1889-9
摘要	Stem cell research has become a hot topic in biology, as the understanding of stem cell biology can provide new insights for both regenerative medicine and clinical treatment of diseases. Accurately deciphering the fate of stem cells is the basis for understanding the mechanism and function of stem cells during tissue repair and regeneration. Cre-loxP-mediated recombination has been widely applied in fate mapping of stem cells for many years. However, nonspecific labeling by conventional cell lineage tracing strategies has led to discrepancies or even controversies in multiple fields. Recently, dual recombinase-mediated lineage tracing strategies have been developed to improve both the resolution and precision of stem cell fate mapping. These new genetic strategies also expand the application of lineage tracing in studying cell origin and fate. Here, we review cell lineage tracing methods, especially dual genetic approaches, and then provide examples to describe how they are used to study stem cell fate plasticity and function in vivo.
关键词	lineage tracing dual recombination Cre-loxP Dre-rox stem cell cell fate plasticity tissue regeneration
收录类别	SCIE
语种	英语
WOS研究方向	Life Sciences & Biomedicine - Other Topics
WOS类目	Biology
WOS记录号	WOS:000639752100001
出版者	SCIENCE PRESS
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/126524
专题	生命科学与技术学院_特聘教授组_周斌组
通讯作者	Zhou, Bin
作者单位	1.Chinese Acad Sci, Univ Chinese Acad Sci, Ctr Excellence Mol Cell Sci, State Key Lab Cell Biol,Shanghai Inst Biochem & C, Shanghai 200031, Peoples R China; 2.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China; 3.Univ Chinese Acad Sci, Hangzhou Inst Adv Study, Sch Life Sci, Hangzhou 310024, Peoples R China
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Jin, Hengwei,Liu, Kuo,Zhou, Bin. Dual recombinases-based genetic lineage tracing for stem cell research with enhanced precision[J]. SCIENCE CHINA-LIFE SCIENCES,2021.
APA	Jin, Hengwei,Liu, Kuo,&Zhou, Bin.(2021).Dual recombinases-based genetic lineage tracing for stem cell research with enhanced precision.SCIENCE CHINA-LIFE SCIENCES.
MLA	Jin, Hengwei,et al."Dual recombinases-based genetic lineage tracing for stem cell research with enhanced precision".SCIENCE CHINA-LIFE SCIENCES (2021).