Phytophthora sojae eeffector Avr1d functions as an E2 competitor and inhibits ubiquitination activity of GmPUB13 to facilitate infection

doi:10.1073/pnas.2018312118

	Phytophthora sojae eeffector Avr1d functions as an E2 competitor and inhibits ubiquitination activity of GmPUB13 to facilitate infection
	Lin, Yachun 1,2,3; Hu, Qinli 4,5,6; Zhou, Jia 4,5,6; Yin, Weixiao 1,7,8; Yao, Deqiang9 ; Shao, Yuanyuan 1; Zhao, Yao 1,2,3; Guo, Baodian 1,2,3; Xia, Yeqiang 1,2,3; Chen, Qian 10,11; Wang, Yan 1,2,3; Ye, Wenwu 1,2,3; Xie, Qi 11; Tyler, Brett M.12; Xing, Weiman 13; Wang, Yuanchao 1,2,3
	2021-03-09
发表期刊	PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (IF:9.4[JCR-2023],10.8[5-Year])
ISSN	0027-8424
卷号	118 期号:10
发表状态	已发表
DOI	10.1073/pnas.2018312118
摘要	Oomycete pathogens such as Phytophthora secrete a repertoire of effectors into host cells to manipulate host immunity and benefit infection. In this study, we found that an RxLR effector, Avr1d, promoted Phytophthora sojae infection in soybean hairy roots. Using a yeast two-hybrid screen, we identified the soybean E3 ubiquitin ligase GmPUB13 as a host target for Avr1d. By coimmunoprecipitation (Co-IP), gel infiltration, and isothermal titration calorimetry (ITC) assays, we confirmed that Avr1d interacts with GmPUB13 both in vivo and in vitro. Furthermore, we found that Avr1d inhibits the E3 ligase activity of GmPUB13. The crystal structure Avr1d in complex with GmPUB13 was solved and revealed that Avr1d occupies the binding site for E2 ubiquitin conjugating enzyme on GmPUB13. In line with this, Avr1d competed with E2 ubiquitin conjugating enzymes for GmPUB13 binding in vitro, thereby decreasing the E3 ligase activity of GmPUB13. Meanwhile, we found that inactivation of the ubiquitin ligase activity of GmPUB13 stabilized GmPUB13 by blocking GmPUB13 degradation. Silencing of GmPUB13 in soybean hairy roots decreased P. sojae infection, suggesting that GmPUB13 acts as a susceptibility factor. Altogether, this study highlights a virulence mechanism of Phytophthora effectors, by which Avr1d competes with E2 for GmPUB13 binding to repress the GmPUB13 E3 ligase activity and thereby stabilizing the susceptibility factor GmPUB13 to facilitate Phytophthora infection. This study unravels the structural basis for modulation of host targets by Phytophthora effectors and will be instrumental for boosting plant resistance breeding.
关键词	effector U-box self-ubiquitination crystal structure susceptibility factor
URL	查看原文
收录类别	SCI ; SCIE
语种	英语
WOS研究方向	Multidisciplinary Sciences
WOS类目	Science & Technology - Other Topics
WOS记录号	WOS:000627429100053
出版者	NATL ACAD SCIENCES
WOS关键词	RXLR EFFECTOR ; PATHOGEN ; IMMUNITY ; SUSCEPTIBILITY ; CHEMOTAXIS ; AVRPTOB
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/126097
专题	iHuman研究所_科学装置(X)_膜蛋白同步辐射线站
通讯作者	Wang, Yan; Xing, Weiman
作者单位	1.Nanjing Agr Univ, Dept Plant Pathol, Nanjing 210095, Peoples R China; 2.Nanjing Agr Univ, Key Lab Integrated Management Crop Dis & Pests, Minist Educ, Nanjing 210095, Peoples R China; 3.Nanjing Agr Univ, Key Lab Plant Immun, Nanjing 210095, Peoples R China; 4.Chinese Acad Sci, Shanghai Ctr Plant Stress Biol, Shanghai 200032, Peoples R China; 5.Chinese Acad Sci, Ctr Excellence Mol Plant Sci, Shanghai 200032, Peoples R China; 6.Univ Chinese Acad Sci, Beijing 100049, Peoples R China; 7.Huazhong Agr Univ, Coll Plant Sci & Technol, Dept Plant Pathol, Wuhan 430070, Peoples R China; 8.Huazhong Agr Univ, Key Lab Crop Dis Monitoring & Safety Control Hube, Wuhan 430070, Peoples R China; 9.ShanghaiTech Univ, iHuman Inst, Shanghai 201210, Peoples R China; 10.China Agr Univ, Coll Plant Protect, Dept Plant Pathol, Minist Agr,Key Lab Pest Monitoring & Green Manage, Beijing 100193, Peoples R China; 11.Chinese Acad Sci, Innovat Acad Seed Design, Inst Genet & Dev Biol, State Key Lab Plant Genom, Beijing 100101, Peoples R China; 12.Oregon State Univ, Ctr Genome Res & Biocomp, Dept Bot & Plant Pathol, Corvallis, OR 97331 USA; 13.Shanghai Normal Univ, Coll Life Sci, Shanghai Key Lab Plant Mol Sci, Shanghai 200234, Peoples R China
推荐引用方式 GB/T 7714	Lin, Yachun,Hu, Qinli,Zhou, Jia,et al. Phytophthora sojae eeffector Avr1d functions as an E2 competitor and inhibits ubiquitination activity of GmPUB13 to facilitate infection[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2021,118(10).
APA	Lin, Yachun.,Hu, Qinli.,Zhou, Jia.,Yin, Weixiao.,Yao, Deqiang.,...&Wang, Yuanchao.(2021).Phytophthora sojae eeffector Avr1d functions as an E2 competitor and inhibits ubiquitination activity of GmPUB13 to facilitate infection.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,118(10).
MLA	Lin, Yachun,et al."Phytophthora sojae eeffector Avr1d functions as an E2 competitor and inhibits ubiquitination activity of GmPUB13 to facilitate infection".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 118.10(2021).