Avidity-Based Selection of Tissue-Specific CAR-T Cells from a Combinatorial Cellular Library of CARs

doi:10.1002/advs.202003091

	Avidity-Based Selection of Tissue-Specific CAR-T Cells from a Combinatorial Cellular Library of CARs
	Ma, Peixiang1 ; Ren, Ping1 ; Zhang, Chuyue1,2,3,4 ; Tang, Jiaxing1,2,3,4 ; Yu, Zheng1 ; Zhu, Xuekai1 ; Fan, Kun1,2,3,4 ; Li, Guanglei2 ; Zhu, Wei1 ; Sang, Wei 5; Min, Chenyu 6; Chen, Wenzhang1 ; Huang, Xingxu2 ; Yang, Guang1 ; Lerner, Richard A.7
	2021-03
发表期刊	ADVANCED SCIENCE (IF:14.3[JCR-2023],16.3[5-Year])
EISSN	2198-3844
卷号	8 期号:6 页码:#VALUE!
DOI	10.1002/advs.202003091
摘要	Using T-cell chimeric antigen receptors (CAR-T) to activate and redirect T cells to tumors expressing the cognate antigen represents a powerful approach in cancer therapy. However, normal tissues with low expression of tumor-associated antigens (TAAs) can be mistargeted, resulting in severe side effects. An approach using a collection of T cells expressing a diverse, 10(6)-member combinatorial cellular library of CARs, in which members can be specifically enriched based on avidity for cell membrane antigens, is reported. Using CD38 as the target antigen, an efficient and effective selection of CARs specifically recognizing CD38(+) tumor cells is demonstrated. These selected CAR-T's produce cytokines known to be associated with T cell activation in a CD38 expression-dependent manner. This avidity-based selection endows the engineered T cells with minimal off-tumor effects, while retaining robust antitumor efficacy both in vitro and in vivo. The described method may facilitate the application of CAR-T therapy to TAAs previously considered undruggable.
关键词	CD38 chimeric antigen receptor combinatorial antibody library tumor-associated antigen
收录类别	SCI ; EI ; SCIE
语种	英语
WOS研究方向	Chemistry ; Science & Technology - Other Topics ; Materials Science
WOS类目	Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary
WOS记录号	WOS:000612641000001
出版者	WILEY
WOS关键词	ANTIBODY-LIKE IMMUNORECEPTORS ; HUMAN CD38 ; AFFINITY ; PERSISTENCE ; ACTIVATION ; RECEPTORS ; CLEAVAGE ; NOTCH ; CYTOTOXICITY ; EXPRESSION
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/125856
专题	免疫化学研究所_特聘教授组_功能筛选实验室生命科学与技术学院_PI研究组_黄行许组免疫化学研究所_特聘教授组_抗体设计学实验室免疫化学研究所_公共科研平台_分析化学平台免疫化学研究所_PI研究组_朱学锴组 T细胞工程实验室生命科学与技术学院_硕士生生命科学与技术学院_博士生
通讯作者	Yang, Guang; Lerner, Richard A.
作者单位	1.ShanghaiTech Univ, Shanghai Inst Adv Immunochem Studies, Shanghai 201210, Peoples R China; 2.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China; 3.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China; 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China; 5.Xuzhou Med Univ, Inst Hematol, Affiliated Hosp, Dept Hematol, Xuzhou 221000, Jiangsu, Peoples R China; 6.Velox Pharmaceut, Changzhou 213000, Jiangsu, Peoples R China; 7.Scripps Res Inst, Dept Chem, La Jolla, CA 92037 USA
第一作者单位	免疫化学研究所
通讯作者单位	免疫化学研究所
第一作者的第一单位	免疫化学研究所
推荐引用方式 GB/T 7714	Ma, Peixiang,Ren, Ping,Zhang, Chuyue,et al. Avidity-Based Selection of Tissue-Specific CAR-T Cells from a Combinatorial Cellular Library of CARs[J]. ADVANCED SCIENCE,2021,8(6):#VALUE!.
APA	Ma, Peixiang.,Ren, Ping.,Zhang, Chuyue.,Tang, Jiaxing.,Yu, Zheng.,...&Lerner, Richard A..(2021).Avidity-Based Selection of Tissue-Specific CAR-T Cells from a Combinatorial Cellular Library of CARs.ADVANCED SCIENCE,8(6),#VALUE!.
MLA	Ma, Peixiang,et al."Avidity-Based Selection of Tissue-Specific CAR-T Cells from a Combinatorial Cellular Library of CARs".ADVANCED SCIENCE 8.6(2021):#VALUE!.