Development of a Broadly Applicable Cas12a-Linked Beam Unlocking Reaction for Sensitive and Specific Detection o f Respiratory Pathogens Including SARS-CoV-2

doi:10.1021/acschembio.0c00840

	Development of a Broadly Applicable Cas12a-Linked Beam Unlocking Reaction for Sensitive and Specific Detection o f Respiratory Pathogens Including SARS-CoV-2
	Wang, Yu1,2 ; Liu, Donglan 3; Lin, Huihuang 4,5; Chen, Dingbin 3; Sun, Jing 3; Xie, Yusang 4,5; Wang, Xinjie1 ; Ma, Peixiang1 ; Nie, Yan1 ; Mei, Hong1 ; Zhao, Baihui 6; Huang, Xingxu7 ; Jiang, Ge1 ; Jiang, Xianxing 2; Qu, Jieming 4,5; Zhao, Jincun 3; Liu, Jia1,3,7
	2021-03-19
发表期刊	ACS CHEMICAL BIOLOGY (IF:3.5[JCR-2023],3.9[5-Year])
ISSN	1554-8929
EISSN	1554-8937
卷号	16 期号:3 页码:491-500
DOI	10.1021/acschembio.0c00840
摘要	The outbreak of novel coronavirus SARS-CoV-2 has caused a worldwide threat to public health. COVID-19 patients with SARS-CoV-2 infection can develop clinical symptoms that are often confused with the infections of other respiratory pathogens. Sensitive and specific detection of SARS-CoV-2 with the ability to discriminate from other viruses is urgently needed for COVID-19 diagnosis. Herein, we streamlined a highly efficient CRISPR-Cas12a-based nucleic acid detection platform, termed Cas12a-linked beam unlocking reaction (CALIBURN). We show that CALIBURN could detect SARS-CoV-2 and other coronaviruses and influenza viruses with little cross-reactivity. Importantly, CALIBURN allowed accurate diagnosis of dinical samples with extremely low viral loads, which is a major obstacle for the clinical applications of existing CRISPR diagnostic platforms. When tested on the specimens from SARS-CoV-2-positive and negative donors, CALIBURN exhibited 73.0% positive and 19.0% presumptive positive rates and 100% specificity. Moreover, unlike existing CRISPR detection methods that were mainly restricted to respiratory specimens, CALIBURN displayed consistent performance across both respiratory and nonrespiratory specimens, suggesting its broad specimen compatibility. Finally, using a mouse model of SARS-CoV-2 infection, we demonstrated that CALIBURN allowed detection of coexisting pathogens without cross-reactivity from a single tissue specimen. Our results suggest that CALIBURN can serve as a versatile platform for the diagnosis of COVID-19 and other respiratory infectious diseases.
收录类别	SCI ; SCIE
语种	英语
WOS研究方向	Biochemistry & Molecular Biology
WOS类目	Biochemistry & Molecular Biology
WOS记录号	WOS:000631431900007
出版者	AMER CHEMICAL SOC
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/125728
专题	免疫化学研究所_PI研究组_刘佳组生命科学与技术学院_PI研究组_邹燕组生命科学与技术学院_PI研究组_黄行许组免疫化学研究所_特聘教授组_功能筛选实验室物质科学与技术学院_PI研究组_季泉江组国际事务处物质科学与技术学院_博士生免疫化学研究所_公共科研平台_抗体筛选平台
通讯作者	Jiang, Xianxing; Qu, Jieming; Zhao, Jincun; Liu, Jia
作者单位	1.ShanghaiTech Univ, Shanghai Inst Adv Immunochem Studies, Shanghai 201210, Peoples R China; 2.Sun Yat Sen Univ, Sch Pharmaceut Sci, Guangdong Key Lab Chiral Mol & Drug Discovery, Guangzhou 510006, Guangdong, Peoples R China; 3.Guangzhou Med Univ, Natl Clin Res Ctr Resp Dis, Guangzhou Inst Resp Hlth, State Key Lab Resp Dis,Affiliated Hosp 1, Guangzhou 510182, Peoples R China; 4.Shanghai Jiao Tong Univ, Ruijin Hosp, Dept Pulm & Crit Care Med, Shanghai 200025, Peoples R China; 5.Shanghai Jiao Tong Univ, Inst Resp Dis, Sch Med, Shanghai 200025, Peoples R China; 6.Shanghai BioGerm Med Biotechnol Co Ltd, Shanghai 201318, Peoples R China; 7.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China
第一作者单位	免疫化学研究所
通讯作者单位	免疫化学研究所; 生命科学与技术学院
第一作者的第一单位	免疫化学研究所
推荐引用方式 GB/T 7714	Wang, Yu,Liu, Donglan,Lin, Huihuang,et al. Development of a Broadly Applicable Cas12a-Linked Beam Unlocking Reaction for Sensitive and Specific Detection o f Respiratory Pathogens Including SARS-CoV-2[J]. ACS CHEMICAL BIOLOGY,2021,16(3):491-500.
APA	Wang, Yu.,Liu, Donglan.,Lin, Huihuang.,Chen, Dingbin.,Sun, Jing.,...&Liu, Jia.(2021).Development of a Broadly Applicable Cas12a-Linked Beam Unlocking Reaction for Sensitive and Specific Detection o f Respiratory Pathogens Including SARS-CoV-2.ACS CHEMICAL BIOLOGY,16(3),491-500.
MLA	Wang, Yu,et al."Development of a Broadly Applicable Cas12a-Linked Beam Unlocking Reaction for Sensitive and Specific Detection o f Respiratory Pathogens Including SARS-CoV-2".ACS CHEMICAL BIOLOGY 16.3(2021):491-500.