Nuclear Morphological Remodeling in Human Granulocytes Is Linked to Prenylation Independently from Cytoskeleton

doi:10.3390/cells9112509

KMS > 免疫化学研究所 > 特聘教授组 > Nicola Elvassore组

	Nuclear Morphological Remodeling in Human Granulocytes Is Linked to Prenylation Independently from Cytoskeleton
	Martewicz, Sebastian1 ; Luni, Camilla1 ; Zhu, Xi1,2,3 ; Cui, Meihua1 ; Hu, Manli1 ; Qu, Siqi1,2 ; Buratto, Damiano1 ; Yang, Guang1 ; Grespan, Eleonora 4; Elvassore, Nicola1,5,6,7
	2020-11
发表期刊	CELLS
卷号	9 期号:11
发表状态	已发表
DOI	10.3390/cells9112509
摘要	Nuclear shape modulates cell behavior and function, while aberrant nuclear morphologies correlate with pathological phenotype severity. Nevertheless, functions of specific nuclear morphological features and underlying molecular mechanisms remain poorly understood. Here, we investigate a nucleus-intrinsic mechanism driving nuclear lobulation and segmentation concurrent with granulocyte specification, independently from extracellular forces and cytosolic cytoskeleton contributions. Transcriptomic regulation of cholesterol biosynthesis is equally concurrent with nuclear remodeling. Its putative role as a regulatory element is supported by morphological aberrations observed upon pharmacological impairment of several enzymatic steps of the pathway, most prominently the sterol increment 14-reductase activity of laminB-receptor and protein prenylation. Thus, we support the hypothesis of a nuclear-intrinsic mechanism for nuclear shape control with the putative involvement of the recently discovered GGTase III complex. Such process could be independent from or complementary to the better studied cytoskeleton-based nuclear remodeling essential for cell migration in both physiological and pathological contexts such as immune system function and cancer metastasis.
关键词	nuclear morphology granulocyte lobulation nuclear segmentation prenylation GGTase III
URL	查看原文
收录类别	SCI ; SCIE
语种	英语
资助项目	ShanghaiTech University[F-0301-15-009] ; Oak Foundation Award[W1095/OCAY-14-191]
WOS研究方向	Cell Biology
WOS类目	Cell Biology
WOS记录号	WOS:000593518900001
出版者	MDPI
引用统计
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/124692
专题	免疫化学研究所_特聘教授组_生物工程学实验室免疫化学研究所_特聘教授组_干细胞生物学实验室免疫化学研究所_特聘教授组_功能筛选实验室生命科学与技术学院_硕士生生命科学与技术学院_博士生免疫化学研究所_特聘教授组_Michael Levitt组免疫化学研究所_特聘教授组_张宏恺组
通讯作者	Elvassore, Nicola
作者单位	1.ShanghaiTech Univ, Shanghai Inst Adv Immunochem Studies SIAIS, Shanghai 201210, Peoples R China 2.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China 4.CNR, Inst Neurosci, I-35127 Padua, Italy 5.Univ Padua, Dept Ind Engn, I-35131 Padua, Italy 6.Venetian Inst Mol Med, I-35129 Padua, Italy 7.UCL Great Ormond St Inst Child Hlth, Stem Cells & Regenerat Med Sect, London WC1N 1EH, England
第一作者单位	免疫化学研究所
通讯作者单位	免疫化学研究所
第一作者的第一单位	免疫化学研究所
推荐引用方式 GB/T 7714	Martewicz, Sebastian,Luni, Camilla,Zhu, Xi,et al. Nuclear Morphological Remodeling in Human Granulocytes Is Linked to Prenylation Independently from Cytoskeleton[J]. CELLS,2020,9(11).
APA	Martewicz, Sebastian.,Luni, Camilla.,Zhu, Xi.,Cui, Meihua.,Hu, Manli.,...&Elvassore, Nicola.(2020).Nuclear Morphological Remodeling in Human Granulocytes Is Linked to Prenylation Independently from Cytoskeleton.CELLS,9(11).
MLA	Martewicz, Sebastian,et al."Nuclear Morphological Remodeling in Human Granulocytes Is Linked to Prenylation Independently from Cytoskeleton".CELLS 9.11(2020).