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MrHex1 is Required for Woronin Body Formation, Fungal Development and Virulence in Metarhizium robertsii | |
2020-09 | |
发表期刊 | JOURNAL OF FUNGI |
ISSN | 2309-608X |
EISSN | 2309-608X |
卷号 | 6期号:3 |
发表状态 | 已发表 |
DOI | 10.3390/jof6030172 |
摘要 | The Woronin body (WB) is a peroxisome-derived dense-core vesicle, a self-assembling hexagonal crystal of a single protein Hex1. This organelle is specific to the ascomycete fungi belonging to the Pezizomycotina subphylum by functioning in sealing septal pores in response to mycelium damage and the control of cell heterogeneity. We retrieved all available Hex1-domain containing proteins of different fungi from the GenBank database and found considerable length variations among 460 obtained Hex1 proteins. However, a highly conserved Hex1 domain containing 75 amino acid residues with a specific S/A-R/S-L consensus motif for targeting peroxisome is present at the carboxy-terminus of each protein. A homologous Hex1 gene, named MrHex1, was deleted in the entomopathogenic fungus Metarhizium robertsii. It was found that MrHex1 was responsible for WB formation in M. robertsii and involved in sealing septal pores to maintain cell integrity and heterogeneity. Different assays indicated that, relative to the wild-type (WT) strain, increment Mrhex1 demonstrated a growth defect on a solid medium and substantial reductions of conidiation, appressorium formation and topical infectivity against insect hosts. However, there was no obvious virulence difference between WT and mutants during injection of insects. We also found that increment MrHex1 could tolerate different stress conditions like the WT and the gene-rescued mutant of M. robertsii, which is in contrast to the reports of the stress-response defects of the Hex1 null mutants of other fungal species. In addition to revealing the phenotypic/functional alterations of the Hex1 deletion mutants between different pathotype fungi, the results of this study may benefit the understanding of the evolution and WB-control of fungal entomopathogenicity. |
关键词 | Woronin body conidiation stress response appressorium formation virulence Metarhizium robertsii |
收录类别 | SCI ; SCIE |
资助项目 | National Key R&D Programs of China[2017YFD0200402][2018YFA0900502] ; Chinese Academy of Sciences[QYZDJ-SSW-SMC028] |
WOS研究方向 | Microbiology ; Mycology |
WOS类目 | Microbiology ; Mycology |
WOS记录号 | WOS:000581365400001 |
出版者 | MDPI |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/123644 |
专题 | 生命科学与技术学院_硕士生 生命科学与技术学院_特聘教授组_王成树组 生命科学与技术学院_博士生 |
通讯作者 | Wang Chengshu |
作者单位 | 1.CAS Key Laboratory of Insect Developmental and Evolutionary Biology, CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai 200032, China 2.School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China 3.CAS Center for Excellence in Biotic Interactions, University of Chinese Academy of Sciences, Beijing 100049, China |
通讯作者单位 | 生命科学与技术学院 |
推荐引用方式 GB/T 7714 | Tang Guirong,Shang Yanfang,Li Shiqin,et al. MrHex1 is Required for Woronin Body Formation, Fungal Development and Virulence in Metarhizium robertsii[J]. JOURNAL OF FUNGI,2020,6(3). |
APA | Tang Guirong,Shang Yanfang,Li Shiqin,&Wang Chengshu.(2020).MrHex1 is Required for Woronin Body Formation, Fungal Development and Virulence in Metarhizium robertsii.JOURNAL OF FUNGI,6(3). |
MLA | Tang Guirong,et al."MrHex1 is Required for Woronin Body Formation, Fungal Development and Virulence in Metarhizium robertsii".JOURNAL OF FUNGI 6.3(2020). |
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