Simultaneous quantitative assessment of two distinct cell lineages with a nuclear-localized dual genetic reporter

doi:10.1016/j.yjmcc.2020.07.002

	Simultaneous quantitative assessment of two distinct cell lineages with a nuclear-localized dual genetic reporter
	Tang, Muxue 1; Liu, Kuo 1; Jin, Hengwei 1; Li, Yan 1; Zhang, Shaohua 1; Liu, Xiuxiu 1; Han, Ximeng 1; Han, Maoying1,2 ; Zhang, Zhenqian 1; Zhou, Bin1,2,3
	2020-09
发表期刊	JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY (IF:4.9[JCR-2023],4.7[5-Year])
ISSN	0022-2828
卷号	146 页码:60-68
DOI	10.1016/j.yjmcc.2020.07.002
摘要	Genetic lineage tracing has been widely used for studying in vivo cell fate plasticity during embryogenesis, tissue homeostasis, and disease development. Recent applications with multiple site-specific recombinases have been used in complex and sophisticated genetic fate mapping studies. However, the previous multicolor reporters for dual recombinases had limitations of precise in situ quantification of cell number, which is mainly due to the intermingling of cells in condensed tissues. Here, we generated a dual recombinase-mediated nuclear-localized GFP and tdTomato reporter line, which enables clear, simultaneous quantification of two distinct cell lineages in vivo. Combining this dual genetic reporter with Tbx18-Cre and Cdh5-Dre lines, which genetically trace epicardial and endothelial cells, respectively, we obtained high-resolution images for the anatomic distribution of the descendants of these two distinct cell lineages in the valve mesenchyme during development, remodeling, and maturation stages. This new dual genetic reporter is expected to facilitate fate tracing of two cell lineages and their objective quantification in vivo.
关键词	Lineage tracing Heart development Endothelial cell Cre-loxP Dre-rox
收录类别	SCI ; SCIE
语种	英语
资助项目	National Key Research & Development Program of China[2019YFA0110400][2019YFA0802000] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA16010507][XDB19000000] ; National Science Foundation of China[31730112][31625019][91849202][81761138040]
WOS研究方向	Cardiovascular System & Cardiology ; Cell Biology
WOS类目	Cardiac & Cardiovascular Systems ; Cell Biology
WOS记录号	WOS:000571466000007
出版者	ELSEVIER SCI LTD
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/123599
专题	生命科学与技术学院_博士生生命科学与技术学院_特聘教授组_周斌组
通讯作者	Zhou, Bin
作者单位	1.Univ Chinese Acad Sci, Ctr Excellence Mol Cell Sci, Shanghai Inst Biochem & Cell Biol, State Key Lab Cell Biol,Chinese Acad Sci, Shanghai 200031, Peoples R China; 2.Shanghai Tech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China; 3.Univ Chinese Acad Sci, Sch Life Sci, Hangzhou Inst Adv Study, Hangzhou 310024, Peoples R China
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Tang, Muxue,Liu, Kuo,Jin, Hengwei,et al. Simultaneous quantitative assessment of two distinct cell lineages with a nuclear-localized dual genetic reporter[J]. JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY,2020,146:60-68.
APA	Tang, Muxue.,Liu, Kuo.,Jin, Hengwei.,Li, Yan.,Zhang, Shaohua.,...&Zhou, Bin.(2020).Simultaneous quantitative assessment of two distinct cell lineages with a nuclear-localized dual genetic reporter.JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY,146,60-68.
MLA	Tang, Muxue,et al."Simultaneous quantitative assessment of two distinct cell lineages with a nuclear-localized dual genetic reporter".JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY 146(2020):60-68.