ShanghaiTech University Knowledge Management System
| MeCas12a, a Highly Sensitive and Specific System for COVID-19 Detection | |
| 2020-10 | |
| 发表期刊 | SCIENCE ADVANCES (IF:11.7[JCR-2023],13.7[5-Year]) |
| ISSN | 2375-2548 |
| 卷号 | 7期号:20页码:2001300 |
| 发表状态 | 已发表 |
| DOI | 10.1002/advs.202001300 |
| 摘要 | Cas12a-based systems, which detect specific nucleic acids via collateral cleavage of reporter DNA, display huge potentials for rapid diagnosis of infectious diseases. Here, the Manganese-enhanced Cas12a (MeCas12a) system is described, where manganese is used to increase the detection sensitivity up to 13-fold, enabling the detection of target RNAs as low as five copies. MeCas12a is also highly specific, and is able to distinguish between single nucleotide polymorphisms (SNPs) differing by a single nucleotide. MeCas12a can detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in clinical samples and distinguish between SARS-CoV-2 and Middle East respiratory syndrome coronavirus (MERS-CoV) RNA in simulated samples, thus offering an attractive alternative to other methods for the diagnosis of infectious diseases including COVID-19 and MERS. |
| 关键词 | COVID-19 CRISPR-based detection CRISPR Cas12a manganese SARS-CoV-2 |
| 收录类别 | SCI ; SCIE ; EI |
| 资助项目 | National Natural Science Foundation of China[31500632][U19A2011] ; China Evergrande Group[2020GIRHHMS05] ; Shanghai Local Grant[ZJ2020-ZD-004] |
| WOS研究方向 | Chemistry ; Science & Technology - Other Topics ; Materials Science |
| WOS类目 | Chemistry, Multidisciplinary ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary |
| WOS记录号 | WOS:000571914600001 |
| 出版者 | WILEY |
| 引用统计 | 正在获取...
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| 文献类型 | 期刊论文 |
| 条目标识符 | https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/123539 |
| 专题 | 免疫化学研究所_PI研究组_刘佳组 生命科学与技术学院_PI研究组_池天组 生命科学与技术学院_PI研究组_邹燕组 生命科学与技术学院_PI研究组_黄行许组 免疫化学研究所_特聘教授组_功能筛选实验室 免疫化学研究所_特聘教授组_抗体设计学实验室 生命科学与技术学院_硕士生 |
| 共同第一作者 | Meng, Qingzhou; Sun, Baoqing; Zhao, Bing |
| 通讯作者 | Liu, Ming; Huang, Xingxu; Wang, Xinjie |
| 作者单位 | 1.ShanghaiTech Univ, Shanghai Inst Adv Immunochem Studies, Shanghai 201210, Peoples R China; 2.Guangzhou Med Univ, Affiliated Canc Hosp & Inst, 78 Hengzhigang Rd, Guangzhou 510095, Peoples R China; 3.Guangzhou Med Univ, Affiliated Hosp 1, Guangzhou Inst Resp Hlth, Natl Clin Res Ctr Resp Dis,State Key Lab Resp Dis, Guangzhou 510120, Peoples R China; 4.Ctr Dis Control & Prevent, Microbiol Testing Lab, Shanghai Pudong New Area, Shanghai 200136, Peoples R China; 5.South China Normal Univ, Ctr Studies Psychol Applicat, Inst Brain Res & Rehabil, Guangdong Key Lab Mental Hlth & Cognit Sci, Guangzhou 510631, Peoples R China; 6.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China |
| 第一作者单位 | 免疫化学研究所 |
| 通讯作者单位 | 生命科学与技术学院; 免疫化学研究所 |
| 第一作者的第一单位 | 免疫化学研究所 |
| 推荐引用方式 GB/T 7714 | Ma, Peixiang,Meng, Qingzhou,Sun, Baoqing,et al. MeCas12a, a Highly Sensitive and Specific System for COVID-19 Detection[J]. SCIENCE ADVANCES,2020,7(20):2001300. |
| APA | Ma, Peixiang.,Meng, Qingzhou.,Sun, Baoqing.,Zhao, Bing.,Dang, Lu.,...&Wang, Xinjie.(2020).MeCas12a, a Highly Sensitive and Specific System for COVID-19 Detection.SCIENCE ADVANCES,7(20),2001300. |
| MLA | Ma, Peixiang,et al."MeCas12a, a Highly Sensitive and Specific System for COVID-19 Detection".SCIENCE ADVANCES 7.20(2020):2001300. |
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