SOX1 Is Required for the Specification of Rostral Hindbrain Neural Progenitor Cells from Human Embryonic Stem Cells

doi:10.1016/j.isci.2020.101475

	SOX1 Is Required for the Specification of Rostral Hindbrain Neural Progenitor Cells from Human Embryonic Stem Cells
	Liu, Xinyuan 1; Fang, Zhuoqing 1; Wen, Jing 1; Tang, Fan 2; Liao, Bing 2; Jing, Naihe4,5 ; Lai, Dongmei 6; Jin, Ying1,2,3,5
	2020-09-25
发表期刊	ISCIENCE (IF:4.6[JCR-2023],5.0[5-Year])
卷号	23 期号:9
DOI	10.1016/j.isci.2020.101475
摘要	Region-specific neural progenitor cells (NPCs) can be generated from human embryonic stem cells (hESCs) by modulating signaling pathways. However, how intrinsic transcriptional factors contribute to the neural regionalization is not well characterized. Here, we generate region-specific NPCs from hESCs and find that SOX1 is highly expressed in NPCs with the rostral hindbrain identity. Moreover, we find that OTX2 inhibits SOX1 expression, displaying exclusive expression between the two factors. Furthermore, SOX1 knockout (KO) leads to the upregulation of midbrain genes and downregulation of rostral hindbrain genes, indicating that SOX1 is required for specification of rostral hindbrain NPCs. Our SOX1 chromatin immunoprecipitation sequencing analysis reveals that SOX1 binds to the distal region of GBX2 to activate its expression. Overexpression of GBX2 largely abrogates SOX1-KO-induced aberrant gene expression.Taken together, this study uncovers previously unappreciated role of SOX1 in early neural regionalization and provides new information for the precise control of the OTX2/GBX2 interface.
收录类别	SCI ; SCIE
语种	英语
资助项目	Ministry of Science and Technology of China[2016YFA0100100] ; National Natural Science Foundation of China[31730055][31801224][31871373] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDB19020100] ; Innovative Research Team of High-level Local Universities in Shanghai[SSMU-ZLCX20180401]
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000577096400002
出版者	CELL PRESS
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/123535
专题	生命科学与技术学院_特聘教授组_景乃禾组生命科学与技术学院_特聘教授组_金颖组
通讯作者	Lai, Dongmei; Jin, Ying
作者单位	1.Chinese Acad Sci, Univ Chinese Acad Sci, CAS Ctr Excellence Mol Cell Sci, Shanghai Inst Nutr & Hlth,CAS Key Lab Tissue Micr, 320 Yueyang Rd, Shanghai 200031, Peoples R China; 2.Shanghai Jiao Tong Univ, Shanghai Key Lab Reprod Med, Dept Histoembryol Genet & Dev Biol, Sch Med, 225 South Chongqing Rd, Shanghai 200025, Peoples R China; 3.Shanghai Jiao Tong Univ, Sch Med, Renji Hosp, Basic Clin Res Ctr, 160 Pujian Rd, Shanghai 200127, Peoples R China; 4.Chinese Acad Sci, Univ Chinese Acad Sci, State Key Lab Cell Biol, CAS Ctr Excellence Mol Cell Sci,Shanghai Inst Bio, 320 Yue Yang Rd, Shanghai 200031, Peoples R China; 5.ShanghaiTech Univ, Sch Life Sci & Technol, 100 Haike Rd, Shanghai 201210, Peoples R China; 6.Shanghai Jiao Tong Univ, Sch Med, Int Peace Matern & Child Hlth Hosp, Shanghai 200030, Peoples R China
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Liu, Xinyuan,Fang, Zhuoqing,Wen, Jing,et al. SOX1 Is Required for the Specification of Rostral Hindbrain Neural Progenitor Cells from Human Embryonic Stem Cells[J]. ISCIENCE,2020,23(9).
APA	Liu, Xinyuan.,Fang, Zhuoqing.,Wen, Jing.,Tang, Fan.,Liao, Bing.,...&Jin, Ying.(2020).SOX1 Is Required for the Specification of Rostral Hindbrain Neural Progenitor Cells from Human Embryonic Stem Cells.ISCIENCE,23(9).
MLA	Liu, Xinyuan,et al."SOX1 Is Required for the Specification of Rostral Hindbrain Neural Progenitor Cells from Human Embryonic Stem Cells".ISCIENCE 23.9(2020).