Structural basis of trehalose recycling by the ABC transporter LpqY-SugABC
2020-10
发表期刊SCIENCE ADVANCES
ISSN2375-2548
卷号6期号:44页码:eabb9833
发表状态已发表
DOI10.1126/sciadv.abb9833
摘要

In bacteria, adenosine 5'-triphosphate (ATP)-binding cassette (ABC) importers are essential for the uptake of nutrients including the nonreducing disaccharide trehalose, a metabolite that is crucial for the survival and virulence of several human pathogens including Mycobacterium tuberculosis. SugABC is an ABC transporter that translocates trehalose from the periplasmic lipoprotein LpqY into the cytoplasm of mycobacteria. Here, we report four highresolution cryo-electron microscopy structures of the mycobacterial LpqY-SugABC complex to reveal how it binds and passes trehalose through the membrane to the cytoplasm. A unique feature observed in this system is the initial mode of capture of the trehalose at the LpqY interface. Uptake is achieved by a pivotal rotation of LpqY relative to SugABC, moving from an open and accessible conformation to a clamped conformation upon trehalose binding. These findings enrich our understanding as to how ABC transporters facilitate substrate transport across the membrane in Gram-positive bacteria.

收录类别SCI ; SCIE ; EI
资助项目National Key Research and Development Program of China[2017YFC0840300] ; National Natural Science Foundation of China[81520108019]
WOS研究方向Science & Technology - Other Topics
WOS类目Multidisciplinary Sciences
WOS记录号WOS:000583031400022
出版者AMER ASSOC ADVANCEMENT SCIENCE
引用统计
文献类型期刊论文
条目标识符https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/123363
专题免疫化学研究所_PI研究组_杨海涛组
免疫化学研究所_特聘教授组_饶子和组
生命科学与技术学院_硕士生
生命科学与技术学院_博士生
生命科学与技术学院_PI研究组_许文青组
共同第一作者Liang, Jingxi; Zhang, Bing; Gao, Yan
通讯作者Zhang, Bing; Rao, Zihe
作者单位
1.Shanghai Institute for Advanced Immunochemical Studies and School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China.
2.CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai 200031, China.
3.University of Chinese Academy of Sciences, Beijing 100101, China.
4.State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin 300353, China.
5.Laboratory of Structural Biology, Tsinghua University, Beijing 100084, China.
6.School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, QLD 4072, Australia.
7.National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.
第一作者单位免疫化学研究所
通讯作者单位免疫化学研究所
第一作者的第一单位免疫化学研究所
推荐引用方式
GB/T 7714
Liu, Fengjiang,Liang, Jingxi,Zhang, Bing,et al. Structural basis of trehalose recycling by the ABC transporter LpqY-SugABC[J]. SCIENCE ADVANCES,2020,6(44):eabb9833.
APA Liu, Fengjiang.,Liang, Jingxi.,Zhang, Bing.,Gao, Yan.,Yang, Xiuna.,...&Rao, Zihe.(2020).Structural basis of trehalose recycling by the ABC transporter LpqY-SugABC.SCIENCE ADVANCES,6(44),eabb9833.
MLA Liu, Fengjiang,et al."Structural basis of trehalose recycling by the ABC transporter LpqY-SugABC".SCIENCE ADVANCES 6.44(2020):eabb9833.
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