ShanghaiTech University Knowledge Management System
Structural basis of trehalose recycling by the ABC transporter LpqY-SugABC | |
2020-10 | |
发表期刊 | SCIENCE ADVANCES |
ISSN | 2375-2548 |
卷号 | 6期号:44页码:eabb9833 |
发表状态 | 已发表 |
DOI | 10.1126/sciadv.abb9833 |
摘要 | In bacteria, adenosine 5'-triphosphate (ATP)-binding cassette (ABC) importers are essential for the uptake of nutrients including the nonreducing disaccharide trehalose, a metabolite that is crucial for the survival and virulence of several human pathogens including Mycobacterium tuberculosis. SugABC is an ABC transporter that translocates trehalose from the periplasmic lipoprotein LpqY into the cytoplasm of mycobacteria. Here, we report four highresolution cryo-electron microscopy structures of the mycobacterial LpqY-SugABC complex to reveal how it binds and passes trehalose through the membrane to the cytoplasm. A unique feature observed in this system is the initial mode of capture of the trehalose at the LpqY interface. Uptake is achieved by a pivotal rotation of LpqY relative to SugABC, moving from an open and accessible conformation to a clamped conformation upon trehalose binding. These findings enrich our understanding as to how ABC transporters facilitate substrate transport across the membrane in Gram-positive bacteria. |
收录类别 | SCI ; SCIE ; EI |
资助项目 | National Key Research and Development Program of China[2017YFC0840300] ; National Natural Science Foundation of China[81520108019] |
WOS研究方向 | Science & Technology - Other Topics |
WOS类目 | Multidisciplinary Sciences |
WOS记录号 | WOS:000583031400022 |
出版者 | AMER ASSOC ADVANCEMENT SCIENCE |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/123363 |
专题 | 免疫化学研究所_PI研究组_杨海涛组 免疫化学研究所_特聘教授组_饶子和组 生命科学与技术学院_硕士生 生命科学与技术学院_博士生 生命科学与技术学院_PI研究组_许文青组 |
共同第一作者 | Liang, Jingxi; Zhang, Bing; Gao, Yan |
通讯作者 | Zhang, Bing; Rao, Zihe |
作者单位 | 1.Shanghai Institute for Advanced Immunochemical Studies and School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China. 2.CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai 200031, China. 3.University of Chinese Academy of Sciences, Beijing 100101, China. 4.State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin 300353, China. 5.Laboratory of Structural Biology, Tsinghua University, Beijing 100084, China. 6.School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, QLD 4072, Australia. 7.National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China. |
第一作者单位 | 免疫化学研究所 |
通讯作者单位 | 免疫化学研究所 |
第一作者的第一单位 | 免疫化学研究所 |
推荐引用方式 GB/T 7714 | Liu, Fengjiang,Liang, Jingxi,Zhang, Bing,et al. Structural basis of trehalose recycling by the ABC transporter LpqY-SugABC[J]. SCIENCE ADVANCES,2020,6(44):eabb9833. |
APA | Liu, Fengjiang.,Liang, Jingxi.,Zhang, Bing.,Gao, Yan.,Yang, Xiuna.,...&Rao, Zihe.(2020).Structural basis of trehalose recycling by the ABC transporter LpqY-SugABC.SCIENCE ADVANCES,6(44),eabb9833. |
MLA | Liu, Fengjiang,et al."Structural basis of trehalose recycling by the ABC transporter LpqY-SugABC".SCIENCE ADVANCES 6.44(2020):eabb9833. |
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