The evolution of zebrafish RAG2 protein is required for adapting to the elevated body temperature of the higher endothermic vertebrates

doi:10.1038/s41598-020-61019-w

	The evolution of zebrafish RAG2 protein is required for adapting to the elevated body temperature of the higher endothermic vertebrates
	Sun, Ao 1; Xu, Ke 1; Liu, Haifeng 1; Li, Hua 1; Shi, Yaohuang1,2 ; Zhu, Xiaoyan 1; Liang, Tao 1; Li, Xinyue 1; Cao, Xianxia1,2 ; Ji, Yanhong 3; Jiang, Taijiao 4; Xu, Chenqi 1; Liu, Xiaolong1,2
	2020-03-05
发表期刊	SCIENTIFIC REPORTS (IF:3.8[JCR-2023],4.3[5-Year])
ISSN	2045-2322
卷号	10 期号:1
发表状态	已发表
DOI	10.1038/s41598-020-61019-w
摘要	The recombination activating gene (RAG or RAG1/RAG2 complex)-mediated adaptive immune system is a hallmark of jawed vertebrates. It has been reported that RAG originated in invertebrates. However, whether RAG further evolved once it arose in jawed vertebrates remains largely unknown. Here, we found that zebrafish RAG (zRAG) had a lower activity than mouse RAG (mRAG). Intriguingly, the attenuated stability of zebrafish RAG2 (zRAG2), but not zebrafish RAG1, caused the reduced V(D)J recombination efficiency compared to mRAG at 37 degrees C which are the body temperature of most endotherms except birds. Importantly, the lower temperature 28 degrees C, which is the best temperature for zebrafish growth, made the recombination efficiency of zRAG similar to that of mRAG by improving the stability of zRAG2. Consistent with the prementioned observation, the V(D)J recombination of Rag2(KI/KI) mice, which zRAG2 was substituted for mRAG2, was also severely impaired. Unexpectedly, Rag2(KI/KI) mice developed cachexia syndromes accompanied by premature death. Taken together, our findings illustrate that the evolution of zebrafish RAG2 protein is required for adapting to the elevated body temperature of the higher endothermic vertebrates.
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收录类别	SCI ; SCIE
语种	英语
资助项目	National Natural Science Foundation of China[31621003][31530021][31500717][31501193] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDB19000000] ; National Key R&D Program of China[2017YFA0506700]
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000563316000020
出版者	NATURE PUBLISHING GROUP
WOS关键词	V(D)J RECOMBINATION ; ALLOSTERIC REGULATION ; MUTATIONS ; EPISTASIS ; CACHEXIA ; ORIGIN ; EXPRESSION ; INITIATION ; MECHANISM ; ELEMENTS
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/123178
专题	生命科学与技术学院_硕士生生命科学与技术学院_特聘教授组_刘小龙组
通讯作者	Liu, Xiaolong
作者单位	1.Univ Chinese Acad Sci, Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol, Ctr Excellence Mol Cell Sci,State Key Lab Cell Bi, Shanghai 200031, Peoples R China 2.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China 3.Xi An Jiao Tong Univ, Sch Basic Med Sci, Dept Pathogen Biol & Immunol, Hlth Sci Ctr, Xian 710061, Shaanxi, Peoples R China 4.Chinese Acad Med Sci & Peking Union Med Coll, Inst Basic Med Sci, Ctr Syst Med, Beijing 100005, Peoples R China
通讯作者单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Sun, Ao,Xu, Ke,Liu, Haifeng,et al. The evolution of zebrafish RAG2 protein is required for adapting to the elevated body temperature of the higher endothermic vertebrates[J]. SCIENTIFIC REPORTS,2020,10(1).
APA	Sun, Ao.,Xu, Ke.,Liu, Haifeng.,Li, Hua.,Shi, Yaohuang.,...&Liu, Xiaolong.(2020).The evolution of zebrafish RAG2 protein is required for adapting to the elevated body temperature of the higher endothermic vertebrates.SCIENTIFIC REPORTS,10(1).
MLA	Sun, Ao,et al."The evolution of zebrafish RAG2 protein is required for adapting to the elevated body temperature of the higher endothermic vertebrates".SCIENTIFIC REPORTS 10.1(2020).