The E3 Ubiquitin Ligase SYVN1 Ubiquitinates Atlastins to Remodel the Endoplasmic Reticulum Network

doi:10.1016/j.isci.2020.101494

	The E3 Ubiquitin Ligase SYVN1 Ubiquitinates Atlastins to Remodel the Endoplasmic Reticulum Network
	Zhao, Yupeng1,2,4 ; Feng, Zhigang1,3,4 ; Zou, Yan1,3,4 ; Liu, Yanfen1,2,4
	2020-09-25
发表期刊	ISCIENCE (IF:4.6[JCR-2023],5.0[5-Year])
卷号	23 期号:9
DOI	10.1016/j.isci.2020.101494
摘要	Atlastin (ATL) is a class of dynamin-like GTPases shaping endoplasmic reticulum (ER) by mediating homotypic membrane fusion. Defect of ATLs leads to abnormal ER structure and hereditary spastic paraplegia (HSP), a neurodegenerative disease with progressive spasticity. How ATLs are regulated to maintain the ER dynamics is not clear. Here, we found that SYVN1, an E3 ubiquitin ligase on the ER membrane, regulates ER shape and COPII exporting by mediating ubiquitination on ATLs, especially ATL1. ATL1 is ubiquitinated by SYVN1 strongly on K285 and mildly on K287. Ubiquitination on ATL1 does not result in protein degradation but inhibits ATL1 GTPase activity. SYVN1 overexpression compensates the excessive ER network fusion caused by ATL1 overexpression. Accordingly, the role of SYVN1 and ATL1 in regulating ERmorphology is also recapitulated in Caenorhabditis elegans. Taken together, our study reveals a different role of SYVN1 in ER remodeling through mediating ubiquitination on ATLs.
收录类别	SCI ; SCIE
语种	英语
资助项目	NIH Office of Research Infrastructure Programs[P40 OD010440] ; National Natural Science Foundation of China[31570781][31770831] ; National Natural Science Foundation of China from Y. Z.'s lab[31571047]
WOS研究方向	Science & Technology - Other Topics
WOS类目	Multidisciplinary Sciences
WOS记录号	WOS:000577094400002
出版者	CELL PRESS
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/122992
专题	生命科学与技术学院_博士生生命科学与技术学院_PI研究组_邹燕组生命科学与技术学院_PI研究组_刘艳芬组
共同第一作者	Feng, Zhigang
通讯作者	Zou, Yan; Liu, Yanfen
作者单位	1.School of Life Science and Technology, ShanghaiTech University, Shanghai, 201210, China 2.CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai, 200031, China 3.Institute of Neuroscience and State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai, 200031, China 4.University of Chinese Academy of Sciences, Beijing 100049, China
第一作者单位	生命科学与技术学院
通讯作者单位	生命科学与技术学院
第一作者的第一单位	生命科学与技术学院
推荐引用方式 GB/T 7714	Zhao, Yupeng,Feng, Zhigang,Zou, Yan,et al. The E3 Ubiquitin Ligase SYVN1 Ubiquitinates Atlastins to Remodel the Endoplasmic Reticulum Network[J]. ISCIENCE,2020,23(9).
APA	Zhao, Yupeng,Feng, Zhigang,Zou, Yan,&Liu, Yanfen.(2020).The E3 Ubiquitin Ligase SYVN1 Ubiquitinates Atlastins to Remodel the Endoplasmic Reticulum Network.ISCIENCE,23(9).
MLA	Zhao, Yupeng,et al."The E3 Ubiquitin Ligase SYVN1 Ubiquitinates Atlastins to Remodel the Endoplasmic Reticulum Network".ISCIENCE 23.9(2020).