Tubulin Double Helix: Lateral and Longitudinal Curvature Changes of Tubulin Protofilament
2020-09
发表期刊SMALL (IF:13.0[JCR-2023],13.5[5-Year])
ISSN1613-6810
EISSN1613-6829
卷号16期号:37
发表状态已发表
DOI10.1002/smll.202001240
摘要

By virtue of their native structures, tubulin dimers are protein building blocks that are naturally preprogrammed to assemble into microtubules (MTs), which are cytoskeletal polymers. Here, polycation-directed (i.e., electrostatically tunable) assembly of tubulins is demonstrated by conformational changes to the tubulin protofilament in longitudinal and lateral directions, creating tubulin double helices and various tubular architectures. Synchrotron small-angle X-ray scattering and transmission electron microscopy reveal a remarkable range of nanoscale assembly structures: single- and double-layered double-helix tubulin tubules. The phase transitions from MTs to the new assemblies are dependent on the size and concentration of polycations. Two characteristic scales that determine the number of observed phases are the size of polycation compared to the size of tubulin (approximate to 4 nm) and to MT diameter (approximate to 25 nm). This work suggests the feasibility of using polycations that have scissor- and glue-like properties to achieve "programmable breakdown" of protein nanotubes, tearing MTs into double-stranded tubulins and building up previously undiscovered nanostructures. Importantly, a new role of tubulins is defined as 2D shape-controllable building blocks for supramolecular architectures. These findings provide insight into the design of protein-based functional materials, for example, as metallization templates for nanoscale electronic devices, molecular screws, and drug delivery vehicles.

关键词2D shape-control molecular switch programmable breakdown protein nanotubes tubulin double helices
收录类别SCI ; SCIE ; EI
资助项目NRF[2017M2A2A6A01071190] ; US Department of Energy (DOE), Office of Basic Energy Sciences, Division of Materials Sciences and Engineering[DE-FG02-06ER46314] ; US National Science Foundation (NSF)[DMR-1807327] ; NIH[NS-35010] ; [NRF-2018R1A2B3001690] ; [KC30 N11170021]
WOS研究方向Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics
WOS类目Chemistry, Multidisciplinary ; Chemistry, Physical ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied ; Physics, Condensed Matter
WOS记录号WOS:000559291400001
出版者WILEY-V C H VERLAG GMBH
WOS关键词ALPHA-BETA-TUBULIN ; MICROTUBULE ; DNA ; CONDENSATION ; PEPTIDES ; DYNAMICS ; NUMBER ; TAXOL
原始文献类型Article
引用统计
正在获取...
文献类型期刊论文
条目标识符https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/122945
专题物质科学与技术学院_PI研究组_yongjin lee组
通讯作者Choi, Myung Chul
作者单位
1.Korea Adv Inst Sci & Technol KAIST, Dept Bio & Brain Engn, 291 Daehak Ro, Daejeon 34141, South Korea
2.Univ Calif Santa Barbara, Dept Mol Cellular & Dev Biol, Santa Barbara, CA 93106 USA
3.Univ Calif Santa Barbara, Neurosci Res Inst, Santa Barbara, CA 93106 USA
4.Univ Calif Santa Barbara, Mat Res Lab, Santa Barbara, CA 93106 USA
5.Univ Calif Santa Barbara, Dept Mat, Santa Barbara, CA 93106 USA
6.Univ Calif Santa Barbara, Dept Phys, Santa Barbara, CA 93106 USA
7.AMOREPACIFIC Res & Dev Ctr, Yongin 17074, South Korea
8.ShanghaiTech Univ, Pudong 201210, Peoples R China
推荐引用方式
GB/T 7714
Lee, Juncheol,Song, Chaeyeon,Lee, Jimin,et al. Tubulin Double Helix: Lateral and Longitudinal Curvature Changes of Tubulin Protofilament[J]. SMALL,2020,16(37).
APA Lee, Juncheol.,Song, Chaeyeon.,Lee, Jimin.,Miller, Herbert P..,Cho, Hasaeam.,...&Choi, Myung Chul.(2020).Tubulin Double Helix: Lateral and Longitudinal Curvature Changes of Tubulin Protofilament.SMALL,16(37).
MLA Lee, Juncheol,et al."Tubulin Double Helix: Lateral and Longitudinal Curvature Changes of Tubulin Protofilament".SMALL 16.37(2020).
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
个性服务
查看访问统计
谷歌学术
谷歌学术中相似的文章
[Lee, Juncheol]的文章
[Song, Chaeyeon]的文章
[Lee, Jimin]的文章
百度学术
百度学术中相似的文章
[Lee, Juncheol]的文章
[Song, Chaeyeon]的文章
[Lee, Jimin]的文章
必应学术
必应学术中相似的文章
[Lee, Juncheol]的文章
[Song, Chaeyeon]的文章
[Lee, Jimin]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。