An efficient method to isolate lemon derived extracellular vesicles for gastric cancer therapy

doi:10.1186/s12951-020-00656-9

	An efficient method to isolate lemon derived extracellular vesicles for gastric cancer therapy
	Yang, Meng 1; Liu, Xiaoyan2 ; Luo, Qingqiong 1; Xu, Lili 3; Chen, Fuxiang 1,4
	2020-07-20
发表期刊	JOURNAL OF NANOBIOTECHNOLOGY (IF:10.6[JCR-2023],11.4[5-Year])
ISSN	14773155
卷号	18 期号:1
发表状态	已发表
DOI	10.1186/s12951-020-00656-9
摘要	Background: Plant-derived extracellular vesicles (PDEVs) have great potential for clinical applications. Ultracentrifugation, considered the gold standard method for the preparation of PDEVs, is efficacious but time-consuming and highly instrument-dependent. Thus, a rapid and handy method is needed to facilitate the basic researches and clinical applications of PDEVs. Results: In this study, we combined electrophoretic technique with 300 kDa cut-off dialysis bag (named ELD) for the isolation of PDEVs, which was time-saving and needed no special equipment. Using ELD, lemon derived extracellular vesicles (LDEVs) could be isolated from lemon juice. Nanoparticle tracking analysis and transmission electron microscopy confirmed that the method separated intact vesicles with a similar size and number to the standard method-ultracentrifugation. LDEVs caused the gastric cancer cell cycle S-phase arrest and induced cell apoptosis. The anticancer activities of LDEVs on gastric cancer cells were mediated by the generation of reactive oxygen species. In addition, LDEVs were safe and could be remained in gastrointestinal organs. Conclusions: ELD was an efficient method for the isolation of LDEVs, and could be carried out in any routine biological laboratory as no special equipment needed. LDEVs exerted anticancer activities on gastric cancer, indicating the great potentials for clinical application as edible chemotherapeutics delivery vehicle. © 2020 The Author(s).
URL	查看原文
收录类别	EI ; SCIE ; SCI
出版者	BioMed Central
EI入藏号	20203108983764
EI主题词	Cell death ; Centrifugation ; Citrus fruits ; Clinical research ; High resolution transmission electron microscopy ; Laboratories
EI分类号	Biology:461.9 ; Optical Devices and Systems:741.3 ; Chemical Operations:802.3 ; Agricultural Products:821.4
原始文献类型	Journal article (JA)
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/122670
专题	生命科学与技术学院_PI研究组_王华翌组
通讯作者	Xu, Lili; Chen, Fuxiang
作者单位	1.Department of Clinical Laboratory, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai; 200011, China 2.School of Life Science and Technology, Shanghai Tech University, Shanghai; 201210, China 3.Division of Gastroenterology, Zhongshan Hospital, Fudan University, Shanghai; 200032, China 4.Faculty of Medical Laboratory Science, School of Medicine, Shanghai Jiao Tong University, Shanghai; 200025, China
推荐引用方式 GB/T 7714	Yang, Meng,Liu, Xiaoyan,Luo, Qingqiong,et al. An efficient method to isolate lemon derived extracellular vesicles for gastric cancer therapy[J]. JOURNAL OF NANOBIOTECHNOLOGY,2020,18(1).
APA	Yang, Meng,Liu, Xiaoyan,Luo, Qingqiong,Xu, Lili,&Chen, Fuxiang.(2020).An efficient method to isolate lemon derived extracellular vesicles for gastric cancer therapy.JOURNAL OF NANOBIOTECHNOLOGY,18(1).
MLA	Yang, Meng,et al."An efficient method to isolate lemon derived extracellular vesicles for gastric cancer therapy".JOURNAL OF NANOBIOTECHNOLOGY 18.1(2020).