Highly efficient generation of sheep with a definedFecB(B)mutation via adenine base editing

doi:10.1186/s12711-020-00554-6

	Highly efficient generation of sheep with a definedFecB(B)mutation via adenine base editing
	Zhou, Shiwei 1; Ding, Yige 1; Liu, Jiao 1; Liu, Yao 1; Zhao, Xiaoe 2; Li, Guanwei 1; Zhang, Chenguang 1; Li, Chao 1; Wang, Ying 1; Kalds, Peter 1; Gao, Yawei 1; Zong, Bo 1; Huang, Xiaoyu 1; Huang, Shuhong 1; Yu, Honghao 3; Kou, Qifang 4; Petersen, Bjoern 5; Huang, Xingxu6 ; Wang, Xiaolong 1; Ma, Baohua 2; Chen, Yulin 1
	2020-07-01
发表期刊	GENETICS SELECTION EVOLUTION (IF:3.6[JCR-2023],4.1[5-Year])
ISSN	0999-193X
EISSN	1297-9686
卷号	52 期号:1
发表状态	已发表
DOI	10.1186/s12711-020-00554-6
摘要	Base editing has the potential to improve important economic traits in agriculture and can precisely convert single nucleotides in DNA or RNA sequences into minimal double-strand DNA breaks (DSB). Adenine base editors (ABE) have recently emerged as a base editing tool for the conversion of targeted A:T to G:C, but have not yet been used in sheep. ABEmax is one of the latest versions of ABE, which consists of a catalytically-impaired nuclease and a laboratory-evolved DNA-adenosine deaminase. The Booroola fecundity (FecB(B)) mutation (g.A746G, p.Q249R) in thebone morphogenetic protein receptor 1B(BMPR1B) gene influences fecundity in many sheep breeds. In this study, by using ABEmax we successfully obtained lambs with defined point mutations that result in an amino acid substitution (p.Gln249Arg). The efficiency of the defined point mutations was 75% in newborn lambs, since six lambs were heterozygous at theFecB(B)mutation site (g.A746G, p.Q249R), and two lambs were wild-type. We did not detect off-target mutations in the eight edited lambs. Here, we report the validation of the first gene-edited sheep generated by ABE and highlight its potential to improve economically important traits in livestock.
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收录类别	SCI ; SCIE
语种	英语
资助项目	National Natural Science Foundation of China[31772571] ; Local Road Research Board[2017NY-072] ; [NXTS2018-001] ; [2018KJXX-009]
WOS研究方向	Agriculture ; Genetics & Heredity
WOS类目	Agriculture, Dairy & Animal Science ; Genetics & Heredity
WOS记录号	WOS:000546930500001
出版者	BMC
WOS关键词	FECX(G) MUTATIONS ; OVULATION RATE ; BOOROOLA FECB ; GENOMIC DNA ; LITTER SIZE ; BMPR-IB ; GENE ; DEAMINASE ; REPAIR
原始文献类型	Article
引用统计	被引频次：25[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/122477
专题	生命科学与技术学院_PI研究组_黄行许组
通讯作者	Wang, Xiaolong; Ma, Baohua; Chen, Yulin
作者单位	1.Northwest A&F Univ, Coll Anim Sci & Technol, Key Lab Anim Genet Breeding & Reprod Shaanxi Prov, Yangling, Shaanxi, Peoples R China 2.Northwest A&F Univ, Coll Vet Med, Yangling, Shaanxi, Peoples R China 3.Guilin Med Univ, Coll Biotechnol, Guilin, Peoples R China 4.Ningxia Tianyuan Tan Sheep Farm, Hongsibu, Peoples R China 5.Friedrich Loeffler Inst, Inst Farm Anim Genet, Neustadt, Germany 6.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China
推荐引用方式 GB/T 7714	Zhou, Shiwei,Ding, Yige,Liu, Jiao,et al. Highly efficient generation of sheep with a definedFecB(B)mutation via adenine base editing[J]. GENETICS SELECTION EVOLUTION,2020,52(1).
APA	Zhou, Shiwei.,Ding, Yige.,Liu, Jiao.,Liu, Yao.,Zhao, Xiaoe.,...&Chen, Yulin.(2020).Highly efficient generation of sheep with a definedFecB(B)mutation via adenine base editing.GENETICS SELECTION EVOLUTION,52(1).
MLA	Zhou, Shiwei,et al."Highly efficient generation of sheep with a definedFecB(B)mutation via adenine base editing".GENETICS SELECTION EVOLUTION 52.1(2020).