Anti-HIV agent trichosanthin enhances the capabilities of chemokines to stimulate chemotaxis and G protein activation, and this is mediated through interaction of trichosanthin and chemokine receptors
Zhao, J; Ben, LH; Wu, YL; Hu, W; Ling, K; Xin, SM; Nie, HL; Ma, L; Pei, G
1999
发表期刊JOURNAL OF EXPERIMENTAL MEDICINE
ISSN0022-1007
EISSN1540-9538
卷号190期号:1页码:101-111
发表状态已发表
DOI10.1084/jem.190.1.101
摘要

Trichosanthin (TCS), an active protein component isolated from a traditional Chinese medicinal herb Trichosanthes kirilowii, has been shown to inhibit HIV infection and has been applied in clinical treatment of AIDS. The recent development that chemokines and chemokine receptors play important roles in HIV infection led us to investigate the possible functional interaction of TCS with chemokines and their receptors. This study demonstrated that TCS greatly enhanced both RANTES (regulated upon activation, normal T cell expressed and secreted)- and stromal cell-derived factor (SDF)-1 alpha-stimulated chemotaxis (EC50 congruent to 1 nM) in leukocytes (THP-1,Jurkat, and peripheral blood lymphocyte cells) and activation of pertussis toxin-sensitive G proteins (EC50 congruent to 20 nM). TCS also significantly augmented chemokine-stimulated activation of chemokine receptors CCR5 and CXCR4 as well as CCR1, CCR2B, CCR3, and CCR4 transiently expressed in HEK293 cells. A mutant TCS with 4,000-fold lower ribosome-inactivating activity showed similar augmentation activity as wild-type TCS. Moreover, now cytometry demonstrated that the specific association of TCS to the cell membranes required the presence of chemokine receptors, and laser confocal microscopy reveals that TCS was colocalized with chemokine receptors on the membranes. The results from TCS-Sepharose pull-down and TCS and chemokine receptor coimmunoprecipitation and cross-linking experiments demonstrated association of TCS with CCR5. Thus, our data clearly demonstrated that TCS synergizes activities of chemokines to stimulate chemotaxis and G protein activation, and the effects of TCS are likely to be mediated through its interaction with chemokine receptors.

关键词chemokine receptors trichosanthin G proteins chemotaxis HIV
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收录类别SCI
WOS研究方向Immunology ; Research & Experimental Medicine
WOS类目Immunology ; Medicine, Research & Experimental
WOS记录号WOS:000081397500010
WOS关键词DELTA-OPIOID RECEPTOR ; HOMOLOGOUS DESENSITIZATION ; IMMUNODEFICIENCY VIRUSES ; CORECEPTOR ACTIVITY ; SUPPRESSIVE FACTORS ; CARBOXYL-TERMINUS ; ESCHERICHIA-COLI ; CELLS ; CCR5 ; RANTES
原始文献类型Article
引用统计
文献类型期刊论文
条目标识符https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/122009
专题个人在本单位外知识产出
通讯作者Pei, G
作者单位1.Chinese Acad Sci, Shanghai Inst Cell Biol, Shanghai 200031, Peoples R China
2.Chinese Acad Sci, Shanghai Res Ctr Life Sci, Shanghai 200031, Peoples R China
3.Shanghai Med Univ, Natl Lab Med Neurobiol, Shanghai 200032, Peoples R China
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Zhao, J,Ben, LH,Wu, YL,et al. Anti-HIV agent trichosanthin enhances the capabilities of chemokines to stimulate chemotaxis and G protein activation, and this is mediated through interaction of trichosanthin and chemokine receptors[J]. JOURNAL OF EXPERIMENTAL MEDICINE,1999,190(1):101-111.
APA Zhao, J.,Ben, LH.,Wu, YL.,Hu, W.,Ling, K.,...&Pei, G.(1999).Anti-HIV agent trichosanthin enhances the capabilities of chemokines to stimulate chemotaxis and G protein activation, and this is mediated through interaction of trichosanthin and chemokine receptors.JOURNAL OF EXPERIMENTAL MEDICINE,190(1),101-111.
MLA Zhao, J,et al."Anti-HIV agent trichosanthin enhances the capabilities of chemokines to stimulate chemotaxis and G protein activation, and this is mediated through interaction of trichosanthin and chemokine receptors".JOURNAL OF EXPERIMENTAL MEDICINE 190.1(1999):101-111.
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