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Anti-HIV agent trichosanthin enhances the capabilities of chemokines to stimulate chemotaxis and G protein activation, and this is mediated through interaction of trichosanthin and chemokine receptors | |
Zhao, J; Ben, LH; Wu, YL; Hu, W; Ling, K; Xin, SM; Nie, HL; Ma, L; Pei, G | |
1999 | |
发表期刊 | JOURNAL OF EXPERIMENTAL MEDICINE |
ISSN | 0022-1007 |
EISSN | 1540-9538 |
卷号 | 190期号:1页码:101-111 |
发表状态 | 已发表 |
DOI | 10.1084/jem.190.1.101 |
摘要 | Trichosanthin (TCS), an active protein component isolated from a traditional Chinese medicinal herb Trichosanthes kirilowii, has been shown to inhibit HIV infection and has been applied in clinical treatment of AIDS. The recent development that chemokines and chemokine receptors play important roles in HIV infection led us to investigate the possible functional interaction of TCS with chemokines and their receptors. This study demonstrated that TCS greatly enhanced both RANTES (regulated upon activation, normal T cell expressed and secreted)- and stromal cell-derived factor (SDF)-1 alpha-stimulated chemotaxis (EC50 congruent to 1 nM) in leukocytes (THP-1,Jurkat, and peripheral blood lymphocyte cells) and activation of pertussis toxin-sensitive G proteins (EC50 congruent to 20 nM). TCS also significantly augmented chemokine-stimulated activation of chemokine receptors CCR5 and CXCR4 as well as CCR1, CCR2B, CCR3, and CCR4 transiently expressed in HEK293 cells. A mutant TCS with 4,000-fold lower ribosome-inactivating activity showed similar augmentation activity as wild-type TCS. Moreover, now cytometry demonstrated that the specific association of TCS to the cell membranes required the presence of chemokine receptors, and laser confocal microscopy reveals that TCS was colocalized with chemokine receptors on the membranes. The results from TCS-Sepharose pull-down and TCS and chemokine receptor coimmunoprecipitation and cross-linking experiments demonstrated association of TCS with CCR5. Thus, our data clearly demonstrated that TCS synergizes activities of chemokines to stimulate chemotaxis and G protein activation, and the effects of TCS are likely to be mediated through its interaction with chemokine receptors. |
关键词 | chemokine receptors trichosanthin G proteins chemotaxis HIV |
URL | 查看原文 |
收录类别 | SCI |
WOS研究方向 | Immunology ; Research & Experimental Medicine |
WOS类目 | Immunology ; Medicine, Research & Experimental |
WOS记录号 | WOS:000081397500010 |
WOS关键词 | DELTA-OPIOID RECEPTOR ; HOMOLOGOUS DESENSITIZATION ; IMMUNODEFICIENCY VIRUSES ; CORECEPTOR ACTIVITY ; SUPPRESSIVE FACTORS ; CARBOXYL-TERMINUS ; ESCHERICHIA-COLI ; CELLS ; CCR5 ; RANTES |
原始文献类型 | Article |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/122009 |
专题 | 个人在本单位外知识产出 |
通讯作者 | Pei, G |
作者单位 | 1.Chinese Acad Sci, Shanghai Inst Cell Biol, Shanghai 200031, Peoples R China 2.Chinese Acad Sci, Shanghai Res Ctr Life Sci, Shanghai 200031, Peoples R China 3.Shanghai Med Univ, Natl Lab Med Neurobiol, Shanghai 200032, Peoples R China |
推荐引用方式 GB/T 7714 | Zhao, J,Ben, LH,Wu, YL,et al. Anti-HIV agent trichosanthin enhances the capabilities of chemokines to stimulate chemotaxis and G protein activation, and this is mediated through interaction of trichosanthin and chemokine receptors[J]. JOURNAL OF EXPERIMENTAL MEDICINE,1999,190(1):101-111. |
APA | Zhao, J.,Ben, LH.,Wu, YL.,Hu, W.,Ling, K.,...&Pei, G.(1999).Anti-HIV agent trichosanthin enhances the capabilities of chemokines to stimulate chemotaxis and G protein activation, and this is mediated through interaction of trichosanthin and chemokine receptors.JOURNAL OF EXPERIMENTAL MEDICINE,190(1),101-111. |
MLA | Zhao, J,et al."Anti-HIV agent trichosanthin enhances the capabilities of chemokines to stimulate chemotaxis and G protein activation, and this is mediated through interaction of trichosanthin and chemokine receptors".JOURNAL OF EXPERIMENTAL MEDICINE 190.1(1999):101-111. |
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