Application of a 3D Bioprinted Hepatocellular Carcinoma Cell Model in Antitumor Drug Research

doi:10.3389/fonc.2020.00878

	Application of a 3D Bioprinted Hepatocellular Carcinoma Cell Model in Antitumor Drug Research
	Sun, Lejia 1,2; Yang, Huayu 1,2; Wang, Yanan 3,4; Zhang, Xinyu 3,4; Jin, Bao 1,2; Xie, Feihu 1,2; Jin, Yukai 1,2; Pang, Yuan 5,6,7; Zhao, Haitao 1,2; Lu, Xin 1,2; Sang, Xinting 1,2; Zhang, Hongbing 3,4; Lin, Feng 5,6,7; Sun, Wei 5,6,7,8; Huang, Pengyu9,10 ; Mao, Yilei 1,2
	2020-06-03
发表期刊	FRONTIERS IN ONCOLOGY
ISSN	2234-943X
卷号	10
发表状态	已发表
DOI	10.3389/fonc.2020.00878
摘要	The existingin vitromodels for antitumor drug screening have great limitations. Many compounds that inhibit 2D cultured cells do not exhibit the same pharmacological effectsin vivo, thereby wasting human and material resources as well as time during drug development. Therefore, developing new models is critical. The 3D bioprinting technology has greater advantages in constructing human tissue compared with sandwich culture and organoid construction. Here, we used 3D bioprinting technology to construct a 3D model with HepG2 cells (3DP-HepG2). The biological activities of the model were evaluated by immunofluorescence, real-time quantitative PCR, and transcriptome sequencing. Compared with the traditional 2D cultured tumor cells (2D-HepG2), 3DP-HepG2 showed significantly improved expression of tumor-related genes, including ALB, AFP, CD133, IL-8, EpCAM, CD24, and beta-TGF genes. Transcriptome sequencing analysis revealed large differences in gene expression between 3DP-HepG2 and 2D-HepG2, especially genes related to hepatocyte function and tumor. We also compared the effects of antitumor drugs in 3DP-HepG2 and 2D-HepG2, and found that the large differences in drug resistance genes between the models may cause differences in the drugs' pharmacodynamics.
关键词	3D bio-printing liver HCC model drug screening anti-tumor drug development
URL	查看原文
收录类别	SCI ; SCIE
语种	英语
资助项目	Tsinghua University-Peking Union Medical College Hospital Cooperation Project[PTQH201904552]
WOS研究方向	Oncology
WOS类目	Oncology
WOS记录号	WOS:000543201400001
出版者	FRONTIERS MEDIA SA
WOS关键词	IN-VITRO ; AUTOPHAGY
原始文献类型	Article
引用统计
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/121878
专题	生命科学与技术学院_PI研究组_黄鹏羽组
通讯作者	Mao, Yilei
作者单位	1.PUMC, Peking Union Med Coll PUMC Hosp, Dept Liver Surg, Beijing, Peoples R China 2.Chinese Acad Med Sci CAMS, Beijing, Peoples R China 3.PUMC, Inst Basic Med Sci, Dept Physiol, State Key Lab Med Mol Biol,CAMS, Beijing, Peoples R China 4.PUMC, Sch Basic Med, Beijing, Peoples R China 5.Tsinghua Univ, Biomfg Ctr, Dept Mech Engn, Beijing, Peoples R China 6.Biomfg & Rapid Forming Technol Key Lab Beijing, Beijing, Peoples R China 7.Tsinghua Univ, Overseas Expertise Intro Ctr Discipline Innovat, Beijing, Peoples R China 8.Drexel Univ, Dept Mech Engn, Philadelphia, PA 19104 USA 9.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China 10.Chinese Acad Sci, CAS Ctr Excellence Mol Cell Sci, Shanghai, Peoples R China
推荐引用方式 GB/T 7714	Sun, Lejia,Yang, Huayu,Wang, Yanan,et al. Application of a 3D Bioprinted Hepatocellular Carcinoma Cell Model in Antitumor Drug Research[J]. FRONTIERS IN ONCOLOGY,2020,10.
APA	Sun, Lejia.,Yang, Huayu.,Wang, Yanan.,Zhang, Xinyu.,Jin, Bao.,...&Mao, Yilei.(2020).Application of a 3D Bioprinted Hepatocellular Carcinoma Cell Model in Antitumor Drug Research.FRONTIERS IN ONCOLOGY,10.
MLA	Sun, Lejia,et al."Application of a 3D Bioprinted Hepatocellular Carcinoma Cell Model in Antitumor Drug Research".FRONTIERS IN ONCOLOGY 10(2020).