A genetically encoded small-size fluorescent pair reveals allosteric conformational changes of G proteins upon its interaction with GPCRs by fluorescence lifetime based FRET

doi:10.1039/d0cc02691c

KMS > iHuman研究所 > 特聘教授组 > Kurt Wuthrich组

	A genetically encoded small-size fluorescent pair reveals allosteric conformational changes of G proteins upon its interaction with GPCRs by fluorescence lifetime based FRET
	Shi, Pan 1,2; Zhang, Yanan 1,2; Lv, Pei 1,2; Fang, Wei 1,2; Ling, Shenglong 1,2; Guo, Xiaoqi 1,2; Li, Dong 1,2; Liu, Sanling 1,2; Sun, Demeng 1,2; Zhang, Longhua 1,2; Liu, Dongsheng3 ; Zheng, Ji-Shen 1,2; Tian, Changlin 1,2,4,5
	2020-06-28
发表期刊	CHEMICAL COMMUNICATIONS (IF:4.3[JCR-2023],4.4[5-Year])
ISSN	1359-7345
EISSN	1364-548X
卷号	56 期号:51 页码:6941-6944
发表状态	已发表
DOI	10.1039/d0cc02691c
摘要	The dynamics of GPCRs (G protein-coupled receptors) coupling for cognate G proteins play a critical role in signal transduction. Herein, we reported a site-specifically labelled small-sized fluorescent pair 7-HC/FlAsH ((7-hydroxycoumarin-4-yl)-ethylglycine/fluorescein arsenical hairpin) for fluorescence lifetime based FRET (fluorescence resonance energy transfer) to reveal conformational differences of G alpha(i1)(inhibitory G proteins) and G alpha(s)(stimulatory G proteins) upon beta(2)AR (beta(2)-adrenergic receptor) coupling. It offers a new generally applicable method to probe protein dynamic interactions or conformational changes.
收录类别	SCI ; SCIE ; EI
语种	英语
资助项目	National Key Research and Development Project of China[2016YFA0400903][2017YFA0505201][2017YFA0505400] ; National Natural Science Foundation of China[21825703][31971152]
WOS研究方向	Chemistry
WOS类目	Chemistry, Multidisciplinary
WOS记录号	WOS:000542898200002
出版者	ROYAL SOC CHEMISTRY
WOS关键词	ALPHA-SUBUNIT ; ACTIVATION ; NUCLEOTIDE
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/121864
专题	iHuman研究所_特聘教授组_Kurt Wuthrich组
通讯作者	Shi, Pan; Tian, Changlin
作者单位	1.Univ Sci & Technol China, Hefei Natl Lab Phys Sci Microscale, Hefei 230027, Peoples R China 2.Univ Sci & Technol China, Sch Life Sci, Hefei 230027, Peoples R China 3.ShanghaiTech Univ, iHuman Inst, 99 HaiKe Rd, Shanghai 201210, Peoples R China 4.Chinese Acad Sci, High Magnet Field Lab, Hefei 230030, Peoples R China 5.Univ Sci & Technol China, Anhui Lab Adv Photon Sci & Technol, Hefei 230026, Peoples R China
推荐引用方式 GB/T 7714	Shi, Pan,Zhang, Yanan,Lv, Pei,et al. A genetically encoded small-size fluorescent pair reveals allosteric conformational changes of G proteins upon its interaction with GPCRs by fluorescence lifetime based FRET[J]. CHEMICAL COMMUNICATIONS,2020,56(51):6941-6944.
APA	Shi, Pan.,Zhang, Yanan.,Lv, Pei.,Fang, Wei.,Ling, Shenglong.,...&Tian, Changlin.(2020).A genetically encoded small-size fluorescent pair reveals allosteric conformational changes of G proteins upon its interaction with GPCRs by fluorescence lifetime based FRET.CHEMICAL COMMUNICATIONS,56(51),6941-6944.
MLA	Shi, Pan,et al."A genetically encoded small-size fluorescent pair reveals allosteric conformational changes of G proteins upon its interaction with GPCRs by fluorescence lifetime based FRET".CHEMICAL COMMUNICATIONS 56.51(2020):6941-6944.