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Mediator MED23 plays opposing roles in directing smooth muscle cell and adipocyte differentiation | |
2012 | |
发表期刊 | GENES & DEVELOPMENT |
ISSN | 0890-9369 |
卷号 | 26期号:19页码:2192-2205 |
发表状态 | 已发表 |
DOI | 10.1101/gad.192666.112 |
摘要 | The Mediator complex functions as a control center, orchestrating diverse signaling, gene activities, and biological processes. However, how Mediator subunits determine distinct cell fates remains to be fully elucidated. Here, we show that Mediator MED23 controls the cell fate preference that directs differentiation into smooth muscle cells (SMCs) or adipocytes. Med23 deficiency facilitates SMC differentiation but represses adipocyte differentiation from the multipotent mesenchymal stem cells. Gene profiling revealed that the presence or absence of Med23 oppositely regulates two sets of genes: the RhoA/MAL targeted cytoskeleton/SMC genes and the Ras/ELK1 targeted growth/adipogenic genes. Mechanistically, MED23 favors ELK1-SRF binding to SMC gene promoters for repression, whereas the lack of MED23 favors MAL-SRF binding to SMC gene promoters for activation. Remarkably, the effect of MED23 on SMC differentiation can be recapitulated in zebrafish embryogenesis. Collectively, our data demonstrate the dual, opposing roles for MED23 in regulating the cytoskeleton/SMC and growth/adipogenic gene programs, suggesting its "Ying-Yang'' function in directing adipogenesis versus SMC differentiation. |
关键词 | Mediator complex MED23 MAL ELK1 smooth muscle cell adipocyte differentiation |
URL | 查看原文 |
WOS研究方向 | Cell Biology ; Developmental Biology ; Genetics & Heredity |
WOS类目 | Cell Biology ; Developmental Biology ; Genetics & Heredity |
WOS记录号 | WOS:000309514100007 |
WOS关键词 | SERUM RESPONSE FACTOR ; GENE-EXPRESSION ; MYOCARDIN FAMILY ; TRANSCRIPTIONAL COACTIVATORS ; SIGNAL-TRANSDUCTION ; ADIPOSE-TISSUE ; TARGET GENE ; STEM-CELLS ; COMPLEX ; SRF |
原始文献类型 | Article |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/120793 |
专题 | 个人在本单位外知识产出 |
通讯作者 | Wang, Gang |
作者单位 | 1.Chinese Acad Sci, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, State Key Lab Cell Biol, Shanghai 200031, Peoples R China 2.Univ Med & Dent New Jersey, New Jersey Med Sch, Dept Biochem & Mol Biol, Newark, NJ 07103 USA 3.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Hlth Sci, Lab Vasc Biol,Key Lab Stem Cell Biol, Shanghai 200025, Peoples R China 4.Nanjing Univ, Model Anim Res Ctr, MOE Key Lab Model Anim Dis Study, Nanjing 210061, Jiangsu, Peoples R China 5.Chinese Acad Sci, Shanghai Inst Biol Sci, CAS MPG Partner Inst Computat Biol, Key Lab Computat Biol, Shanghai 200031, Peoples R China 6.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Informat Ctr Life Sci, Shanghai 200031, Peoples R China |
推荐引用方式 GB/T 7714 | Yin, Jing-wen,Liang, Yan,Park, Ji Yeon,et al. Mediator MED23 plays opposing roles in directing smooth muscle cell and adipocyte differentiation[J]. GENES & DEVELOPMENT,2012,26(19):2192-2205. |
APA | Yin, Jing-wen.,Liang, Yan.,Park, Ji Yeon.,Chen, Dongrui.,Yao, Xiao.,...&Wang, Gang.(2012).Mediator MED23 plays opposing roles in directing smooth muscle cell and adipocyte differentiation.GENES & DEVELOPMENT,26(19),2192-2205. |
MLA | Yin, Jing-wen,et al."Mediator MED23 plays opposing roles in directing smooth muscle cell and adipocyte differentiation".GENES & DEVELOPMENT 26.19(2012):2192-2205. |
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