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Autophagy induction stabilizes microtubules and promotes axon regeneration after spinal cord injury | |
2016 | |
发表期刊 | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA |
ISSN | 0027-8424 |
卷号 | 113期号:40页码:11324-11329 |
发表状态 | 已发表 |
DOI | 10.1073/pnas.1611282113 |
摘要 | Remodeling of cytoskeleton structures, such as microtubule assembly, is believed to be crucial for growth cone initiation and regrowth of injured axons. Autophagy plays important roles in maintaining cellular homoeostasis, and its dysfunction causes neuronal degeneration. The role of autophagy in axon regeneration after injury remains speculative. Here we demonstrate a role of autophagy in regulating microtubule dynamics and axon regeneration. We found that autophagy induction promoted neurite outgrowth, attenuated the inhibitory effects of nonpermissive substrate myelin, and decreased the formation of retraction bulbs following axonal injury in cultured cortical neurons. Interestingly, autophagy induction stabilized microtubules by degrading SCG10, a microtubule disassembly protein in neurons. In mice with spinal cord injury, local administration of a specific autophagy-inducing peptide, Tat-beclin1, to lesion sites markedly attenuated axonal retraction of spinal dorsal column axons and cortical spinal tract and promoted regeneration of descending axons following long-term observation. Finally, administration of Tat-beclin1 improved the recovery of motor behaviors of injured mice. These results show a promising effect of an autophagy-inducing reagent on injured axons, providing direct evidence supporting a beneficial role of autophagy in axon regeneration. |
关键词 | autophagy microtubule stabilization axon regeneration |
URL | 查看原文 |
收录类别 | SCI |
语种 | 英语 |
WOS研究方向 | Science & Technology - Other Topics |
WOS类目 | Multidisciplinary Sciences |
WOS记录号 | WOS:000384528900077 |
WOS关键词 | MYELIN-ASSOCIATED GLYCOPROTEIN ; CORTICOSPINAL TRACT REGENERATION ; IN-VIVO ; NEURITE OUTGROWTH ; NOGO RECEPTOR ; GROWTH CONE ; FUNCTIONAL RECOVERY ; DEGENERATING AXONS ; PROTEIN-SYNTHESIS ; GENETIC DELETION |
原始文献类型 | Article |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/120334 |
专题 | 个人在本单位外知识产出 |
共同第一作者 | Ding, Yue-Tong |
通讯作者 | Luo, Zhen-Ge |
作者单位 | 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Neurosci, State Key Lab Neurosci, Shanghai 200031, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Biol Sci, State Key Lab Mol Biol, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China 4.Chinese Acad Sci, Ctr Excellence Brain Sci & Intelligence Technol, Shanghai 200031, Peoples R China |
推荐引用方式 GB/T 7714 | He, Miao,Ding, Yue-Tong,Chu, Chen,et al. Autophagy induction stabilizes microtubules and promotes axon regeneration after spinal cord injury[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2016,113(40):11324-11329. |
APA | He, Miao,Ding, Yue-Tong,Chu, Chen,Tang, Jing,Xiao, Qi,&Luo, Zhen-Ge.(2016).Autophagy induction stabilizes microtubules and promotes axon regeneration after spinal cord injury.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,113(40),11324-11329. |
MLA | He, Miao,et al."Autophagy induction stabilizes microtubules and promotes axon regeneration after spinal cord injury".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 113.40(2016):11324-11329. |
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