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Super-resolution fluorescence imaging of organelles in live cells with photoswitchable membrane probes | |
2012 | |
发表期刊 | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA |
ISSN | 0027-8424 |
卷号 | 109期号:35页码:13978-13983 |
发表状态 | 已发表 |
DOI | 10.1073/pnas.1201882109 |
摘要 | Imaging membranes in live cells with nanometer-scale resolution promises to reveal ultrastructural dynamics of organelles that are essential for cellular functions. In this work, we identified photoswitchable membrane probes and obtained super-resolution fluorescence images of cellular membranes. We demonstrated the photoswitching capabilities of eight commonly used membrane probes, each specific to the plasma membrane, mitochondria, the endoplasmic recticulum (ER) or lysosomes. These small-molecule probes readily label live cells with high probe densities. Using these probes, we achieved dynamic imaging of specific membrane structures in living cells with 30-60 nm spatial resolution at temporal resolutions down to 1-2 s. Moreover, by using spectrally distinguishable probes, we obtained two-color super-resolution images of mitochondria and the ER. We observed previously obscured details of morphological dynamics of mitochondrial fusion/fission and ER remodeling, as well as heterogeneous membrane diffusivity on neuronal processes. |
关键词 | nanoscopy diffraction limit photoswitchable dye stochastic optical reconstruction microscopy photoactivation localization microscopy |
URL | 查看原文 |
收录类别 | SCI |
WOS研究方向 | Science & Technology - Other Topics |
WOS类目 | Multidisciplinary Sciences |
WOS记录号 | WOS:000308565300028 |
WOS关键词 | PHOTOACTIVATED LOCALIZATION MICROSCOPY ; OPTICAL RECONSTRUCTION MICROSCOPY ; SUBDIFFRACTION-RESOLUTION ; MITOCHONDRIAL DYNAMICS ; DIFFRACTION-LIMIT ; LIVING CELLS ; DARK STATES ; NANOSCOPY ; FLUOROPHORES ; STORM |
原始文献类型 | Article |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/120206 |
专题 | 个人在本单位外知识产出 |
通讯作者 | Bi, Guo-Qiang; Zhuang, Xiaowei |
作者单位 | 1.Univ Sci & Technol China, Hefei Natl Lab Phys Sci Microscale, Hefei 230026, Anhui, Peoples R China 2.Univ Sci & Technol China, Sch Life Sci, Hefei 230026, Anhui, Peoples R China 3.Harvard Univ, Dept Chem & Chem Biol, Cambridge, MA 02138 USA 4.Harvard Univ, Howard Hughes Med Inst, Cambridge, MA 02138 USA 5.Harvard Univ, Ctr Brain Sci, Cambridge, MA 02138 USA 6.Harvard Univ, Dept Phys, Cambridge, MA 02138 USA |
推荐引用方式 GB/T 7714 | Shim, Sang-Hee,Xia, Chenglong,Zhong, Guisheng,et al. Super-resolution fluorescence imaging of organelles in live cells with photoswitchable membrane probes[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2012,109(35):13978-13983. |
APA | Shim, Sang-Hee.,Xia, Chenglong.,Zhong, Guisheng.,Babcock, Hazen P..,Vaughan, Joshua C..,...&Zhuang, Xiaowei.(2012).Super-resolution fluorescence imaging of organelles in live cells with photoswitchable membrane probes.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,109(35),13978-13983. |
MLA | Shim, Sang-Hee,et al."Super-resolution fluorescence imaging of organelles in live cells with photoswitchable membrane probes".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 109.35(2012):13978-13983. |
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