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Structural Basis for Dimerization and Activity of Human PAPD1, a Noncanonical Poly(A) Polymerase | |
2011 | |
发表期刊 | MOLECULAR CELL |
ISSN | 1097-2765 |
EISSN | 1097-4164 |
卷号 | 41期号:3页码:311-320 |
发表状态 | 已发表 |
DOI | 10.1016/j.molcel.2011.01.013 |
摘要 | Poly(A) polymerases (PAPs) are found in most living organisms and have important roles in RNA function and metabolism. Here, we report the crystal structure of human PAPD1, a noncanonical PAP that can polyadenylate RNAs in the mitochondria (also known as mtPAP) and oligouridylate histone mRNAs (TUTase1). The overall structure of the palm and fingers domains is similar to that in the canonical PAPs. The active site is located at the interface between the two domains, with a large pocket that can accommodate the substrates. The structure reveals the presence of a previously unrecognized domain in the N-terminal region of PAPD1, with a backbone fold that is similar to that of RNP-type RNA binding domains. This domain (named the RL domain), together with a beta-arm insertion in the palm domain, contributes to dimerization of PAPD1. Surprisingly, our mutagenesis and biochemical studies show that dimerization is required for the catalytic activity of PAPD1. |
URL | 查看原文 |
收录类别 | SCI |
WOS研究方向 | Biochemistry & Molecular Biology ; Cell Biology |
WOS类目 | Biochemistry & Molecular Biology ; Cell Biology |
WOS记录号 | WOS:000287460500009 |
WOS关键词 | RNA-BINDING DOMAIN ; FACTOR-I-M ; MESSENGER-RNA ; MACROMOLECULAR STRUCTURES ; CRYSTAL-STRUCTURE ; PROTEIN ; POLYADENYLATION ; RECOGNITION ; MECHANISM ; IDENTIFICATION |
原始文献类型 | Article |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/120085 |
专题 | 个人在本单位外知识产出 |
通讯作者 | Tong, Liang |
作者单位 | Columbia Univ, Dept Biol Sci, New York, NY 10027 USA |
推荐引用方式 GB/T 7714 | Bai, Yun,Srivastava, Sandeep K.,Chang, Jeong Ho,et al. Structural Basis for Dimerization and Activity of Human PAPD1, a Noncanonical Poly(A) Polymerase[J]. MOLECULAR CELL,2011,41(3):311-320. |
APA | Bai, Yun,Srivastava, Sandeep K.,Chang, Jeong Ho,Manley, James L.,&Tong, Liang.(2011).Structural Basis for Dimerization and Activity of Human PAPD1, a Noncanonical Poly(A) Polymerase.MOLECULAR CELL,41(3),311-320. |
MLA | Bai, Yun,et al."Structural Basis for Dimerization and Activity of Human PAPD1, a Noncanonical Poly(A) Polymerase".MOLECULAR CELL 41.3(2011):311-320. |
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