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Bowl-shaped oligomeric structures on membranes as DegP's new functional forms in protein quality control | |
2009 | |
发表期刊 | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA |
ISSN | 0027-8424 |
卷号 | 106期号:12页码:4858-4863 |
发表状态 | 已发表 |
DOI | 10.1073/pnas.0811780106 |
摘要 | In the periplasm of Escherichia coli, DegP (also known as HtrA), which has both chaperone-like and proteolytic activities, prevents the accumulation of toxic misfolded and unfolded polypeptides. In solution, upon binding to denatured proteins, DegP forms large cage-like structures. Here, we show that DegP forms a range of bowl-shaped structures, independent of substrate proteins, each with a 4-, 5-, or 6-fold symmetry and all with a DegP trimer as the structural unit, on lipid membranes. These membrane-bound DegP assemblies have the capacity to recruit and process substrates in the bowl chamber, and they exhibit higher proteolytic and lower chaperone-like activities than DegP in solution. Our findings imply that DegP might regulate its dual roles during protein quality control, depending on its assembly state in the narrow bacterial envelope. |
关键词 | chaperone protease HtrA cryo-electron microscopy |
URL | 查看原文 |
收录类别 | SCI |
WOS研究方向 | Science & Technology - Other Topics |
WOS类目 | Multidisciplinary Sciences |
WOS记录号 | WOS:000264522600052 |
WOS关键词 | HEAT-SHOCK PROTEASE ; ESCHERICHIA-COLI ; CHAPERONE FUNCTION ; HTRA PROTEASE ; ACTIVATION ; DEGRADATION ; TEMPERATURE ; SUPPRESSES ; ENVELOPE ; STRESS |
原始文献类型 | Article |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/120044 |
专题 | 个人在本单位外知识产出 |
通讯作者 | Sui, Sen-Fang |
作者单位 | 1.Tsinghua Univ, Dept Biol Sci & Biotechnol, State Key Lab Biomembrane & Membrane Biotechnol, Beijing 100084, Peoples R China 2.Lanzhou Univ, Inst Biophys, Lanzhou 730000, Peoples R China 3.Yale Univ, Dept Mol Biophys & Biochem, New Haven, CT 06520 USA |
推荐引用方式 GB/T 7714 | Shen, Qing-Tao,Bai, Xiao-Chen,Chang, Lei-Fu,et al. Bowl-shaped oligomeric structures on membranes as DegP's new functional forms in protein quality control[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2009,106(12):4858-4863. |
APA | Shen, Qing-Tao,Bai, Xiao-Chen,Chang, Lei-Fu,Wu, Yi,Wang, Hong-Wei,&Sui, Sen-Fang.(2009).Bowl-shaped oligomeric structures on membranes as DegP's new functional forms in protein quality control.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,106(12),4858-4863. |
MLA | Shen, Qing-Tao,et al."Bowl-shaped oligomeric structures on membranes as DegP's new functional forms in protein quality control".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 106.12(2009):4858-4863. |
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