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Nudel and FAK as Antagonizing Strength Modulators of Nascent Adhesions through Paxillin | |
2009 | |
发表期刊 | PLOS BIOLOGY |
ISSN | 1545-7885 |
卷号 | 7期号:5 |
发表状态 | 已发表 |
DOI | 10.1371/journal.pbio.1000116 |
摘要 | Adhesion and detachment are coordinated critical steps during cell migration. Conceptually, efficient migration requires both effective stabilization of membrane protrusions at the leading edge via nascent adhesions and their successful persistence during retraction of the trailing side via disruption of focal adhesions. As nascent adhesions are much smaller in size than focal adhesions, they are expected to exhibit a stronger adhesivity in order to achieve the coordination between cell front and back. Here, we show that Nudel knockdown by interference RNA (RNAi) resulted in cell edge shrinkage due to poor adhesions of membrane protrusions. Nudel bound to paxillin, a scaffold protein of focal contacts, and colocalized with it in areas of active membrane protrusions, presumably at nascent adhesions. The Nudel-paxillin interaction was disrupted by focal adhesion kinase (FAK) in a paxillin-binding-dependent manner. Forced localization of Nudel in all focal contacts by fusing it to paxillin markedly strengthened their adhesivity, whereas overexpression of structurally activated FAK or any paxillin-binding FAK mutant lacking the N-terminal autoinhibitory domain caused cell edge shrinkage. These results suggest a novel mechanism for selective reinforcement of nascent adhesions via interplays of Nudel and FAK with paxillin to facilitate cell migration. |
URL | 查看原文 |
收录类别 | SCI |
语种 | 英语 |
WOS研究方向 | Biochemistry & Molecular Biology ; Life Sciences & Biomedicine - Other Topics |
WOS类目 | Biochemistry & Molecular Biology ; Biology |
WOS记录号 | WOS:000267282000014 |
出版者 | PUBLIC LIBRARY SCIENCE |
WOS关键词 | NEURONAL MIGRATION DEFECTS ; EARLY EMBRYONIC LETHALITY ; CELL-MIGRATION ; CYTOPLASMIC DYNEIN ; FOCAL ADHESIONS ; LEADING-EDGE ; REGULATES ADHESION ; RHO-GTPASES ; TGF-BETA ; PROTEIN |
原始文献类型 | Article |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/119886 |
专题 | 个人在本单位外知识产出 |
通讯作者 | Shan, Yongli; Zhu, Xueliang |
作者单位 | Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Mol Cell Biol Lab, Shanghai, Peoples R China |
推荐引用方式 GB/T 7714 | Shan, Yongli,Yu, Lihou,Li, Yan,et al. Nudel and FAK as Antagonizing Strength Modulators of Nascent Adhesions through Paxillin[J]. PLOS BIOLOGY,2009,7(5). |
APA | Shan, Yongli.,Yu, Lihou.,Li, Yan.,Pan, Youdong.,Zhang, Qiangge.,...&Zhu, Xueliang.(2009).Nudel and FAK as Antagonizing Strength Modulators of Nascent Adhesions through Paxillin.PLOS BIOLOGY,7(5). |
MLA | Shan, Yongli,et al."Nudel and FAK as Antagonizing Strength Modulators of Nascent Adhesions through Paxillin".PLOS BIOLOGY 7.5(2009). |
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