Structure and assembly of the SF3a splicing factor complex of U2 snRNP

doi:10.1038/emboj.2012.7

	Structure and assembly of the SF3a splicing factor complex of U2 snRNP
	Lin, Pei-Chun ; Xu, Rui-Ming
	2012
发表期刊	EMBO JOURNAL
ISSN	0261-4189
EISSN	1460-2075
卷号	31 期号:6 页码:1579-159
发表状态	已发表
DOI	10.1038/emboj.2012.7
摘要	SF3a is an evolutionarily conserved heterotrimeric complex essential for pre-mRNA splicing. It functions in spliceosome assembly within the mature U2 snRNP (small nuclear ribonucleoprotein particle), and its displacement from the spliceosome initiates the first step of the splicing reaction. We have identified a core domain of the yeast SF3a complex required for complex assembly and determined its crystal structure. The structure shows a bifurcated assembly of three subunits, Prp9, Prp11 and Prp21, with Prp9 interacting with Prp21 via a bidentate-binding mode, and Prp21 wrapping around Prp11. Structure-guided biochemical analysis also shows that Prp9 harbours a major binding site for stem-loop IIa of U2 snRNA. These findings provide mechanistic insights into the assembly of U2 snRNP. The EMBO Journal (2012) 31, 1579-1590. doi:10.1038/emboj.2012.7; Published online 7 February 2012
关键词	protein structure RNA splicing U2 snRNP X-ray crystallography
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收录类别	SCI
WOS研究方向	Biochemistry & Molecular Biology ; Cell Biology
WOS类目	Biochemistry & Molecular Biology ; Cell Biology
WOS记录号	WOS:000302131600021
出版者	WILEY
WOS关键词	SMALL NUCLEAR RIBONUCLEOPROTEIN ; CRYSTAL-STRUCTURE ; SACCHAROMYCES-CEREVISIAE ; YEAST ; PROTEIN ; SPLICEOSOME ; PRP9 ; DOMAINS ; BINDING ; MOTIF
原始文献类型	Article
引用统计
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/119814
专题	个人在本单位外知识产出
通讯作者	Xu, Rui-Ming
作者单位	1.Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100101, Peoples R China 2.NYU, Sch Med, Skirball Inst Biomol Med, Struct Biol Program,Kimmel Ctr Biol & Med, New York, NY USA 3.NYU, Sch Med, Dept Pharmacol, New York, NY USA
推荐引用方式 GB/T 7714	Lin, Pei-Chun,Xu, Rui-Ming. Structure and assembly of the SF3a splicing factor complex of U2 snRNP[J]. EMBO JOURNAL,2012,31(6):1579-159.
APA	Lin, Pei-Chun,&Xu, Rui-Ming.(2012).Structure and assembly of the SF3a splicing factor complex of U2 snRNP.EMBO JOURNAL,31(6),1579-159.
MLA	Lin, Pei-Chun,et al."Structure and assembly of the SF3a splicing factor complex of U2 snRNP".EMBO JOURNAL 31.6(2012):1579-159.