Survival Motor Neuron Protein Participates in Mouse Germ Cell Development and Spermatogonium Maintenance

doi:10.3390/ijms21030794

	Survival Motor Neuron Protein Participates in Mouse Germ Cell Development and Spermatogonium Maintenance
	Chang, Wei-Fang 1; Xu, Jie 2; Lin, Tzu-Ying 1; Hsu, Jing 1; Hsieh-Li, Hsiu-Mei 3; Hwu, Yuh-Ming 4,5,6; Liu, Ji-Long7,8 ; Lu, Chung-Hao 4; Sung, Li-Ying 1,9,10
	2020-02
发表期刊	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (IF:4.9[JCR-2023],5.6[5-Year])
EISSN	1422-0067
卷号	21 期号:3
发表状态	已发表
DOI	10.3390/ijms21030794
摘要	The defective human survival motor neuron 1 (SMN1) gene leads to spinal muscular atrophy (SMA), the most common genetic cause of infant mortality. We previously reported that loss of SMN results in rapid differentiation of Drosophila germline stem cells and mouse embryonic stem cells (ESCs), indicating that SMN also plays important roles in germ cell development and stem cell biology. Here, we show that in healthy mice, SMN is highly expressed in the gonadal tissues, prepubertal spermatogonia, and adult spermatocytes, whereas low SMN expression is found in differentiated spermatid and sperm. In SMA-like mice, the growth of testis tissues is retarded, accompanied with gamete development abnormalities and loss of the spermatogonia-specific marker. Consistently, knockdown of Smn1 in spermatogonial stem cells (SSCs) leads to a compromised regeneration capacity in vitro and in vivo in transplantation experiments. In SMA-like mice, apoptosis and accumulation of the R-loop structure were significantly elevated, indicating that SMN plays a critical role in the survival of male germ cells. The present work demonstrates that SMN, in addition to its critical roles in neuronal development, participates in mouse germ cell and spermatogonium maintenance.
关键词	SMN gametogenesis spermatogonium
URL	查看原文
收录类别	SCI ; SCIE
语种	英语
资助项目	Ministry of Science and Technology, Taiwan[106-2313-B-002-039-MY3] ; Ministry of Science and Technology, Taiwan[106-2314-B-195-006-MY3] ; Ministry of Science and Technology, Taiwan[108-2811-B-002-582]
WOS研究方向	Biochemistry & Molecular Biology ; Chemistry
WOS类目	Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
WOS记录号	WOS:000522551602007
出版者	MDPI
WOS关键词	STEM-CELLS ; R-LOOPS ; SMN DEFICIENCY ; PLZF ; SPERMATOGENESIS ; GROWTH ; MODEL ; GENE ; TRANSPLANTATION ; REPERTOIRE
原始文献类型	Article
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/119470
专题	生命科学与技术学院_PI研究组_刘冀珑组
通讯作者	Lu, Chung-Hao; Sung, Li-Ying
作者单位	1.Natl Taiwan Univ, Inst Biotechnol, Taipei 106, Taiwan 2.Univ Michigan, Med Ctr, Ctr Adv Models Translat Sci & Therapeut, 1500 E Med Ctr Dr, Ann Arbor, MI 48109 USA 3.Natl Taiwan Normal Univ, Dept Life Sci, Taipei 116, Taiwan 4.Mackay Mem Hosp, Dept Obstet & Gynecol, Taipei 10449, Taiwan 5.Mackay Med Coll, Dept Obstet & Gynecol, New Taipei 252, Taiwan 6.Mackay Jr Coll Med Nursing & Management, Dept Obstet & Gynecol, Taipei 11260, Taiwan 7.Univ Oxford, MRC Funct Genom Unit, Dept Physiol Anat & Genet, Oxford OX1 2JD, England 8.Shanghai Tech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China 9.Acad Sinica, Agr Biotechnol Res Ctr, Taipei 115, Taiwan 10.Natl Taiwan Univ, Anim Resource Ctr, Taipei 106, Taiwan
推荐引用方式 GB/T 7714	Chang, Wei-Fang,Xu, Jie,Lin, Tzu-Ying,et al. Survival Motor Neuron Protein Participates in Mouse Germ Cell Development and Spermatogonium Maintenance[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2020,21(3).
APA	Chang, Wei-Fang.,Xu, Jie.,Lin, Tzu-Ying.,Hsu, Jing.,Hsieh-Li, Hsiu-Mei.,...&Sung, Li-Ying.(2020).Survival Motor Neuron Protein Participates in Mouse Germ Cell Development and Spermatogonium Maintenance.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,21(3).
MLA	Chang, Wei-Fang,et al."Survival Motor Neuron Protein Participates in Mouse Germ Cell Development and Spermatogonium Maintenance".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 21.3(2020).