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Structure of the RNA-dependent RNA polymerase from COVID-19 virus | |
Gao, Yan1,2,3,4; Yan, Liming1,2; Huang, Yucen1,2; Liu, Fengjiang3,4; Zhao, Yao3,4; Cao, Lin5,6; Wang, Tao1,2; Sun, Qianqian3,4; Ming, Zhenhua7; Zhang, Lianqi1,2; Ge, Ji1,2; Zheng, Litao1,2; Zhang, Ying1,2; Wang, Haofeng3,4,8; Zhu, Yan3,4; Zhu, Chen3,4; Hu, Tianyu3,4; Hua, Tian3,4; Zhang, Bing3,4; Yang, Xiuna3,4; Li, Jun3,4; Yang, Haitao3,4; Liu, Zhijie3,4; Xu, Wenqing3,4; Guddat, Luke W.9; Wang, Quan3,4; Lou, Zhiyong1,2; Rao, Zihe1,2,3,4,5,6,10
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2020-05-15 | |
发表期刊 | SCIENCE |
ISSN | 0036-8075 |
EISSN | 1095-9203 |
卷号 | 368期号:6492页码:779-+ |
发表状态 | 已发表 |
DOI | 10.1126/science.abb7498 |
摘要 | A novel coronavirus [severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2)] outbreak has caused a global coronavirus disease 2019 (COVID-19) pandemic, resulting in tens of thousands of infections and thousands of deaths worldwide. The RNA-dependent RNA polymerase [(RdRp), also named nsp12] is the central component of coronaviral replication and transcription machinery, and it appears to be a primary target for the antiviral drug remdesivir. We report the cryo-electron microscopy structure of COVID-19 virus full-length nsp12 in complex with cofactors nsp7 and nsp8 at 2.9-angstrom resolution. In addition to the conserved architecture of the polymerase core of the viral polymerase family, nsp12 possesses a newly identified b-hairpin domain at its N terminus. A comparative analysis model shows how remdesivir binds to this polymerase. The structure provides a basis for the design of new antiviral therapeutics that target viral RdRp. |
收录类别 | SCI ; SCIE |
资助项目 | National Program on Key Research Project of China[2017YFC0840300][2020YFA0707500] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDB08020200] ; National Natural Science Foundation of China[81520108019][813300237] ; Science and Technology Commission of Shanghai Municipality[20431900200] |
WOS研究方向 | Science & Technology - Other Topics |
WOS类目 | Multidisciplinary Sciences |
WOS记录号 | WOS:000535608300049 |
出版者 | AMER ASSOC ADVANCEMENT SCIENCE |
WOS关键词 | REPLICATION ; PROTEIN |
原始文献类型 | Article |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/119225 |
专题 | 生命科学与技术学院_硕士生 免疫化学研究所_特聘教授组_饶子和组 iHuman研究所_公共科研平台_生命科学电镜平台 iHuman研究所_PI研究组_刘志杰组 生命科学与技术学院_博士生 生命科学与技术学院_PI研究组_许文青组 免疫化学研究所_PI研究组_杨海涛组 免疫化学研究所_PI研究组_王权组 iHuman研究所_PI研究组_华甜组 免疫化学研究所_PI研究组_李俊组 |
共同第一作者 | Yan, Liming; Huang, Yucen; Liu, Fengjiang |
通讯作者 | Wang, Quan; Lou, Zhiyong; Rao, Zihe |
作者单位 | 1.Tsinghua Univ, Sch Life Sci, Lab Struct Biol, Beijing, Peoples R China 2.Tsinghua Univ, Sch Med, Beijing, Peoples R China 3.ShanghaiTech Univ, Shanghai Inst Adv Immunochem Studies, Shanghai, Peoples R China 4.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China 5.Nankai Univ, Coll Life Sci, Frontiers Sci Ctr Cell Response, State Key Lab Med Chem Biol, Tianjin, Peoples R China 6.Nankai Univ, Coll Pharm, Tianjin, Peoples R China 7.Guangxi Univ, Coll Life Sci & Technol, State Key Lab Conservat & Utilizat Subtrop Agrobi, Nanning, Peoples R China 8.Tianjin Univ, Sch Life Sci, Tianjin, Peoples R China 9.Univ Queensland, Sch Chem & Mol Biosci, Brisbane, Qld, Australia 10.Chinese Acad Sci, Inst Biophys, CAS Ctr Excellence Biomacromol, Natl Lab Biomacromol, Beijing, Peoples R China |
第一作者单位 | 免疫化学研究所; 生命科学与技术学院 |
通讯作者单位 | 免疫化学研究所; 生命科学与技术学院 |
推荐引用方式 GB/T 7714 | Gao, Yan,Yan, Liming,Huang, Yucen,et al. Structure of the RNA-dependent RNA polymerase from COVID-19 virus[J]. SCIENCE,2020,368(6492):779-+. |
APA | Gao, Yan.,Yan, Liming.,Huang, Yucen.,Liu, Fengjiang.,Zhao, Yao.,...&Rao, Zihe.(2020).Structure of the RNA-dependent RNA polymerase from COVID-19 virus.SCIENCE,368(6492),779-+. |
MLA | Gao, Yan,et al."Structure of the RNA-dependent RNA polymerase from COVID-19 virus".SCIENCE 368.6492(2020):779-+. |
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