Structure of the RNA-dependent RNA polymerase from COVID-19 virus
2020-05-15
发表期刊SCIENCE
ISSN0036-8075
EISSN1095-9203
卷号368期号:6492页码:779-+
发表状态已发表
DOI10.1126/science.abb7498
摘要

A novel coronavirus [severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2)] outbreak has caused a global coronavirus disease 2019 (COVID-19) pandemic, resulting in tens of thousands of infections and thousands of deaths worldwide. The RNA-dependent RNA polymerase [(RdRp), also named nsp12] is the central component of coronaviral replication and transcription machinery, and it appears to be a primary target for the antiviral drug remdesivir. We report the cryo-electron microscopy structure of COVID-19 virus full-length nsp12 in complex with cofactors nsp7 and nsp8 at 2.9-angstrom resolution. In addition to the conserved architecture of the polymerase core of the viral polymerase family, nsp12 possesses a newly identified b-hairpin domain at its N terminus. A comparative analysis model shows how remdesivir binds to this polymerase. The structure provides a basis for the design of new antiviral therapeutics that target viral RdRp.

收录类别SCI ; SCIE
资助项目National Program on Key Research Project of China[2017YFC0840300][2020YFA0707500] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDB08020200] ; National Natural Science Foundation of China[81520108019][813300237] ; Science and Technology Commission of Shanghai Municipality[20431900200]
WOS研究方向Science & Technology - Other Topics
WOS类目Multidisciplinary Sciences
WOS记录号WOS:000535608300049
出版者AMER ASSOC ADVANCEMENT SCIENCE
WOS关键词REPLICATION ; PROTEIN
原始文献类型Article
引用统计
文献类型期刊论文
条目标识符https://kms.shanghaitech.edu.cn/handle/2MSLDSTB/119225
专题生命科学与技术学院_硕士生
免疫化学研究所_特聘教授组_饶子和组
iHuman研究所_公共科研平台_生命科学电镜平台
iHuman研究所_PI研究组_刘志杰组
生命科学与技术学院_博士生
生命科学与技术学院_PI研究组_许文青组
免疫化学研究所_PI研究组_杨海涛组
免疫化学研究所_PI研究组_王权组
iHuman研究所_PI研究组_华甜组
免疫化学研究所_PI研究组_李俊组
共同第一作者Yan, Liming; Huang, Yucen; Liu, Fengjiang
通讯作者Wang, Quan; Lou, Zhiyong; Rao, Zihe
作者单位
1.Tsinghua Univ, Sch Life Sci, Lab Struct Biol, Beijing, Peoples R China
2.Tsinghua Univ, Sch Med, Beijing, Peoples R China
3.ShanghaiTech Univ, Shanghai Inst Adv Immunochem Studies, Shanghai, Peoples R China
4.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China
5.Nankai Univ, Coll Life Sci, Frontiers Sci Ctr Cell Response, State Key Lab Med Chem Biol, Tianjin, Peoples R China
6.Nankai Univ, Coll Pharm, Tianjin, Peoples R China
7.Guangxi Univ, Coll Life Sci & Technol, State Key Lab Conservat & Utilizat Subtrop Agrobi, Nanning, Peoples R China
8.Tianjin Univ, Sch Life Sci, Tianjin, Peoples R China
9.Univ Queensland, Sch Chem & Mol Biosci, Brisbane, Qld, Australia
10.Chinese Acad Sci, Inst Biophys, CAS Ctr Excellence Biomacromol, Natl Lab Biomacromol, Beijing, Peoples R China
第一作者单位免疫化学研究所;  生命科学与技术学院
通讯作者单位免疫化学研究所;  生命科学与技术学院
推荐引用方式
GB/T 7714
Gao, Yan,Yan, Liming,Huang, Yucen,et al. Structure of the RNA-dependent RNA polymerase from COVID-19 virus[J]. SCIENCE,2020,368(6492):779-+.
APA Gao, Yan.,Yan, Liming.,Huang, Yucen.,Liu, Fengjiang.,Zhao, Yao.,...&Rao, Zihe.(2020).Structure of the RNA-dependent RNA polymerase from COVID-19 virus.SCIENCE,368(6492),779-+.
MLA Gao, Yan,et al."Structure of the RNA-dependent RNA polymerase from COVID-19 virus".SCIENCE 368.6492(2020):779-+.
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